Thomas Lee Ortel

Journal article Blood · November 1, 2004 Featured Publication Annexin A5 (A5) forms 2-dimensional crystals over phospholipid bilayers, blocking their availability for coagulation reactions. Recently, human antiphospholipid (aPL) monoclonal antibodies (mAbs) have been demonstrated by atomic force microscopy (AFM) to d ... Full text Link to item Cite

Regulatable aptamers in medicine: focus on antithrombotic strategies.

Journal article Expert Opin Biol Ther · October 2004 Featured Publication Proteins generally execute the key physiological activities required for normal growth and homeostasis. As such, many different classes of proteins, including proteases, kinases, cellular receptors and signalling proteins, represent attractive targets for ... Full text Link to item Cite

Use of a new platelet function analyzer to detect von Willebrand disease in women with menorrhagia.

Journal article Am J Obstet Gynecol · August 2004 Featured Publication OBJECTIVE: The purpose of this study was to assess the usefulness of a new platelet function analyzer in the detection of von Willebrand disease and platelet dysfunction in women with menorrhagia. STUDY DESIGN: Women with menorrhagia and control subjects w ... Full text Link to item Cite

A review of the therapeutic uses of thrombin.

Journal article Thromb Haemost · May 2004 Featured Publication Thrombin is the product of the hemostatic response essential to the conversion of fibrinogen to fibrin. In addition, it is also responsible for the aggregation of blood platelets in the formation of the "platelet plug" as well as the activation of factor V ... Full text Link to item Cite

Hypercoagulable states and antithrombotic strategies in recurrent vascular access site thrombosis.

Journal article J Vasc Surg · September 2003 Featured Publication Vascular access site thrombosis is a major cause of morbidity in patients receiving hemodialysis. The role of hypercoagulable states in recurrent vascular access site thrombosis remains poorly understood. Data are limited regarding systemic anticoagulation ... Full text Link to item Cite

Contributions of Asn2198, Met2199, and Phe2200 in the factor VIII C2 domain to cofactor activity, phospholipid-binding, and von Willebrand factor-binding.

Journal article Thromb Haemost · May 2003 Featured Publication The crystal structure of the factor VIII C2 domain consists of a beta-sandwich core from which beta-hairpins and loops extend to form a hydrophobic surface. The hydrophobic surface includes M2199 and F2200 at the tip of the 1(st) beta-hairpin. To determine ... Link to item Cite

Anti-beta(2)-glycoprotein I antibody-mediated inhibition of activated protein C requires binding of beta(2)-glycoprotein I to phospholipids.

Journal article Thromb Haemost · October 2002 Featured Publication To clarify the role(s) of anti-beta(2) GPI antibodies on thrombosis in anti-phospholipid antibody syndromes (APS), the effect of IgG from three patients on activated protein C (APC) was investigated using phospholipid vesicles and purified proteins. Two of ... Link to item Cite

RNA aptamers as reversible antagonists of coagulation factor IXa.

Journal article Nature · September 5, 2002 Featured Publication Many therapeutic agents are associated with adverse effects in patients. Anticoagulants can engender acute complications such as significant bleeding that increases patient morbidity and mortality. Antidote control provides the safest means to regulate dru ... Full text Link to item Cite

Antiphospholipid antibodies after surgical exposure to topical bovine thrombin.

Journal article J Lab Clin Med · June 2002 Featured Publication Exposure to topical bovine thrombin during surgery frequently results in the development of antibodies to multiple protein and carbohydrate antigens. We investigated the frequency of increased levels of antibodies to cardiolipin and beta(2)-glycoprotein I ... Full text Link to item Cite

Two classes of germline genes both derived from the V(H)1 family direct the formation of human antibodies that recognize distinct antigenic sites in the C2 domain of factor VIII.

Journal article Blood · April 15, 2002 Featured Publication Most plasmas from patients with inhibitors contain antibodies that are reactive with the C2 domain of factor VIII. Previously, we have shown that the variable heavy chain (V(H)) regions of antibodies to the C2 domain are encoded by the closely related germ ... Full text Link to item Cite

Risk factors associated with thrombosis in patients with antiphospholipid antibodies.

Journal article J Rheumatol · September 2001 Featured Publication OBJECTIVE: To define risk factors associated with thrombosis in patients with antiphospholipid antibodies (aPL). METHODS: Ninety-nine patients with aPL, most of whom had prior thrombosis, were evaluated for the presence of acquired and inherited thrombophi ... Link to item Cite

Fine mapping of inhibitory anti-factor V antibodies using factor V C2 domain mutants. Identification of two antigenic epitopes involved in phospholipid binding.

Journal article Thromb Haemost · June 2001 Featured Publication Hemorrhagic factor V inhibitors frequently bind to the second C-type (C2) domain of factor V and interfere with phospholipid binding. To define specific residues recognized by inhibitors from four patients (one bovine thrombin-induced and three spontaneous ... Link to item Cite

Factor VIII Arg2304 --> His binds to phosphatidylserine and is a functional cofactor in the factor X-ase complex.

Journal article Thromb Haemost · February 2001 Featured Publication Four factor VIII light chain constructs containing hemophilia A mutations at R2304 and R2307 were prepared and expressed in mammalian cells. These mutations are located in a putative phosphatidylserine binding site identified by peptide studies (spanning a ... Link to item Cite

Immunologic impact and clinical outcomes after surgical exposure to bovine thrombin.

Journal article Ann Surg · January 2001 Featured Publication OBJECTIVE: To determine prospectively the immunologic response and adverse clinical events in surgical patients exposed to bovine thrombin during cardiac surgical procedures. SUMMARY BACKGROUND DATA: Topical bovine thrombin is used extensively as a hemosta ... Full text Link to item Cite

Familial antiphospholipid antibody syndrome: criteria for disease and evidence for autosomal dominant inheritance.

Journal article Arthritis Rheum · February 1999 Featured Publication OBJECTIVE: To develop diagnostic criteria for a familial form of antiphospholipid antibody syndrome (APS), identify families with >1 affected member, examine possible modes of inheritance, and determine linkage to potential candidate genes. METHODS: Family ... Full text Link to item Cite