Scholars@Duke
Pengfei Song

Pengfei Song

Associate Professor of Biomedical Engineering
Biomedical Engineering
1427 Fitzpatrick Center, Box 90281, Durham, NC 27708

Scholarly Works - Preprints

Longitudinal Awake Mouse Brain Imaging Using Functional Ultrasound and Functional Ultrasound Localization Microscopy

Preprint · 2026 Ultrafast ultrasound offers a unique route to cross-scale neurovascular phenotyping by integrating functional ultrasound (fUS), ultrasound localization microscopy (ULM), and functional ULM (fULM). Yet the baseline variability, longitudinal stability, and b ... Full text Cite

Three-Dimensional Photoacoustic Tomography with Ultrasound Localization Priors

Preprint · 2026 Three-dimensional photoacoustic tomography (3D-PAT) enables noninvasive structural and functional imaging with optical absorption contrast. However, its spatial resolution is limited by acoustic diffraction and incomplete detection geometry. Here, ... Full text Cite

Functional Ultrasound Localization Microscopy on Freely Moving Rats

Preprint · 2026 AbstractBrain-wide functional neuroimaging at single-vessel resolution in naturally behaving animals has been challenging to realize. Functional ultrasound localization microscopy (fULM) offers brain-wide hemodynamic imaging at microsco ... Full text Cite

Longitudinal awake imaging of mouse deep brain microvasculature with super-resolution ultrasound localization microscopy.

Preprint · November 2025 Ultrasound localization microscopy (ULM) is an emerging imaging modality that resolves microvasculature in deep tissues with high spatial resolution. However, existing preclinical ULM applications are largely constrained to anesthetized animals, introducin ... Full text Cite

Longitudinal Awake Imaging of Mouse Deep Brain Microvasculature with Super-resolution Ultrasound Localization Microscopy

Preprint · October 3, 2025 AbstractUltrasound localization microscopy (ULM) is an emerging imaging modality that resolves microvasculature in deep tissues with high spatial resolution. However, existing preclinical ULM applications are large ... Full text Cite

Microbubble Track-based Functional Ultrasound Localization Microscopy in Awake Mice.

Preprint · August 22, 2025 Functional neuroimaging with ultrafast ultrasound is an emerging neuroimaging tool for studying neural activities in the rodent brain. Existing methods, however, are challenged by the compromise between functional imaging sensitivity (i.e., sensitivity in ... Full text Link to item Cite

Microbubble Backscattering Intensity Improves the Sensitivity of Three-dimensional (3D) Functional Ultrasound Localization Microscopy (fULM)

Preprint · 2025 Functional ultrasound localization microscopy (fULM) enables brain-wide mapping of neural activity at micron-scale resolution but suffers from limited sensitivity due to sparse and noisy microbubble (MB) detections. Extending fULM into three dimensions (3D ... Full text Cite

Longitudinal Awake Imaging of Deep Mouse Brain Microvasculature with Super-resolution Ultrasound Localization Microscopy

Preprint · March 21, 2024 AbstractSuper-resolution ultrasound localization microscopy (ULM) is an emerging imaging modality that resolves capillary-scale microvasculature in deep tissues. However, existing preclinical ULM applications are l ... Full text Cite

Combined Nanodrops Imaging and Ultrasound Localization Microscopy for Detecting Intracerebral Hemorrhage

Preprint · 2024 Objective Advanced imaging methods are crucial for understanding stroke mechanisms and discovering effective treatments to reduce bleeding and enhance recovery. In preclinical in vivo stroke imaging, MRI, CT, and optical imaging are commonly use ... Full text Cite

Deep Learning-based Microbubble Localization for Ultrasound Localization Microscopy

Preprint · 2022 Ultrasound localization microscopy (ULM) is an emerging vascular imaging technique that overcomes the resolution-penetration compromise of ultrasound imaging. Accurate and robust microbubble (MB) localization is essential for successful ULM. In this study, ... Full text Cite

Three-dimensional Shear Wave Elastography Using a 2D Row Column Addressing (RCA) Array

Preprint · 2021 Objective To develop a 3D shear wave elastography (SWE) technique using a 2D row column addressing (RCA) array, with either external vibration or acoustic radiation force (ARF) as the shear wave source. Impact Statement The proposed met ... Full text Cite