Phyllis Carolyn Leppert

Journal article Reprod Sci · December 2009 Featured Publication Uterine leiomyoma are common, benign tumors that are enriched in extracellular matrix. The tumors are characterized by a disoriented and loosely packed collagen fibril structure similar to other diseases with disrupted Transforming growth factor beta (TGF- ... Full text Link to item Cite

Predictors of pregnancy in women with polycystic ovary syndrome.

Journal article J Clin Endocrinol Metab · September 2009 Featured Publication CONTEXT: Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility. The selection of first-line therapies for ovulation induction is empiric. OBJECTIVE: The aim of the study was to develop a clinically useful predictive model of ... Full text Link to item Cite

Sample size calculation for microarray experiments with blocked one-way design.

Journal article BMC Bioinformatics · May 28, 2009 Featured Publication BACKGROUND: One of the main objectives of microarray analysis is to identify differentially expressed genes for different types of cells or treatments. Many statistical methods have been proposed to assess the treatment effects in microarray experiments. R ... Full text Link to item Cite

Keeping clinicians in clinical research: the Clinical Research/Reproductive Scientist Training Program.

Journal article Fertil Steril · March 2009 Featured Publication In recent years the need for translational and clinical research has increased, whereas the number of physicians involved in clinical research has diminished. There is clearly a need for formalized academic training in the quantitative and methodologic pri ... Full text Link to item Cite

Ovarian preservation by GnRH agonists during chemotherapy: a meta-analysis.

Journal article J Womens Health (Larchmt) · March 2009 Featured Publication PURPOSE: Treatment with cyclophosphamide (CYC) confers up to a 40% risk of ovarian failure in women of reproductive age. The use of GnRH agonists (GnRHa) to preserve ovarian function has been investigated in several small studies. We performed a systematic ... Full text Link to item Cite

Medication adherence and treatment success in the National Institute of Child Health and Human Development-Reproductive Medicine Network's Pregnancy in Polycystic Ovary Syndrome Trial.

Journal article Fertil Steril · October 2008 Featured Publication We investigated whether poor adherence with metformin tablets may have contributed to the poor success rates seen in the metformin-containing arms of the Pregnancy in Polycystic Ovary Syndrome (PPCOS) Trial. Median adherence for both metformin and clomiphe ... Full text Link to item Cite

Mechanical homeostasis is altered in uterine leiomyoma.

Journal article Am J Obstet Gynecol · April 2008 Featured Publication OBJECTIVE: Uterine leiomyoma produce an extracellular matrix (ECM) that is abnormal in its volume, content, and structure. Alterations in ECM can modify mechanical stress on cells and lead to activation of Rho-dependent signaling and cell growth. Here we s ... Full text Link to item Cite

Ovulatory response to treatment of polycystic ovary syndrome is associated with a polymorphism in the STK11 gene.

Journal article J Clin Endocrinol Metab · March 2008 Featured Publication CONTEXT: Clomiphene and insulin sensitizers such as metformin are used to induce ovulation in polycystic ovary syndrome (PCOS), but the ovulatory response is variable, and the causes of this variation are poorly understood. OBJECTIVE: Our objective was to ... Full text Link to item Cite

Thrombospondin-1 and thrombospondin-2 mRNA and TSP-1 and TSP-2 protein expression in uterine fibroids and correlation to the genes COL1A1 and COL3A1 and to the collagen cross-link hydroxyproline.

Journal article Reprod Sci · December 2007 Featured Publication Uterine fibroids are composed of altered collagen fibrils and represent an arrested response to injury-initiating fibrosis. In many tissues, TSP-1 is secreted by adult macrophages and monocytes upon wounding and is involved in the activation of transformin ... Full text Link to item Cite

Hysterectomy and loss of fertility: implications for women's mental health.

Journal article J Psychosom Res · September 2007 Featured Publication OBJECTIVE: The objective of this study was to assess the percentage of women scheduled for hysterectomy who would have liked to have had a child or more children and associated factors. METHODS: The study sample included 1140 premenopausal women having hys ... Full text Link to item Cite

Progesterone protects fetal chorion and maternal decidua cells from calcium-induced death.

Journal article Am J Obstet Gynecol · March 2007 Featured Publication OBJECTIVE: The purpose of this study was to determine whether progesterone exerts a protective effect in chorion and decidua cells when exposed to calcimycin. STUDY DESIGN: Fetal membrane samples were collected from term elective repeat cesarean deliveries ... Full text Link to item Cite

Clomiphene, metformin, or both for infertility in the polycystic ovary syndrome.

Journal article N Engl J Med · February 8, 2007 Featured Publication BACKGROUND: The polycystic ovary syndrome is a common cause of infertility. Clomiphene and insulin sensitizers are used alone and in combination to induce ovulation, but it is unknown whether one approach is superior. METHODS: We randomly assigned 626 infe ... Full text Link to item Cite

Utility of screening for other causes of infertility in women with "known" polycystic ovary syndrome.

Journal article Fertil Steril · February 2007 Featured Publication We investigated the prevalence of abnormal screening results (questionnaire and testing for other causes of oligo-ovulation, male or tubal factor infertility) in a group of 1,313 oligo-ovulatory women (included and excluded subjects) whose condition was sc ... Full text Link to item Cite

The Pregnancy in Polycystic Ovary Syndrome study: baseline characteristics of the randomized cohort including racial effects.

Journal article Fertil Steril · October 2006 Featured Publication OBJECTIVE: To report the baseline characteristics and racial differences in the polycystic ovary syndrome (PCOS) phenotype from a large multicenter clinical trial (PPCOS). DESIGN: Double-blind, randomized trial of three treatment regimens (with extended re ... Full text Link to item Cite

The promise and perils of microarray analysis.

Journal article Am J Obstet Gynecol · August 2006 Featured Publication Microarray analysis has provided a novel means of identifying clues into the mechanisms of disease development. As a methodology, microarray analysis holds the promise for genome-wide screening in which 2 tissues (diseased and normal) are compared, and mol ... Full text Link to item Cite

A new hypothesis about the origin of uterine fibroids based on gene expression profiling with microarrays.

Journal article Am J Obstet Gynecol · August 2006 Featured Publication This article will discuss some recent insights based on our microarray studies that have emphasized the role the extracellular matrix, transforming growth factor beta, and collagen structure in fibroid formation. These studies led to appreciation of molecu ... Full text Link to item Cite

21st century--Reproductive Medicine Network (RMN).

Journal article Fertil Steril · December 2004 Featured Publication Full text Link to item Cite

Histological dating of timed endometrial biopsy tissue is not related to fertility status.

Journal article Fertil Steril · November 2004 Featured Publication OBJECTIVE: To assess the ability of histological dating to discriminate between women of fertile and infertile couples. The utility of histological dating of endometrium in the evaluation of infertile couples is uncertain. DESIGN: Prospective multicenter s ... Full text Link to item Cite

Absence of secretory endometrium after false-positive home urine luteinizing hormone testing.

Journal article Fertil Steril · November 2004 Featured Publication OBJECTIVE: To examine the proportion of cases with proliferative endometrium on biopsies performed after positive home urine LH testing. DESIGN: Multicenter clinical trial of the usefulness of endometrial biopsy in the evaluation of infertility, with women ... Full text Link to item Cite

Comparative ultrastructure of collagen fibrils in uterine leiomyomas and normal myometrium.

Journal article Fertil Steril · October 2004 Featured Publication OBJECTIVE: To examine the ultrastructural characteristics of extracellular matrix and mature collagen fibrils in uterine leiomyomas and compare them with those in adjacent normal myometrium. DESIGN: Analysis of paired leiomyoma-myometrium in surgical speci ... Full text Link to item Cite

Reduced dermatopontin expression is a molecular link between uterine leiomyomas and keloids.

Journal article Genes Chromosomes Cancer · July 2004 Featured Publication Uterine leiomyomas are prevalent estrogen-responsive clonal tumors, but the specific genetic alterations that contribute to their development have not been elucidated. To identify genes involved in the formation of leiomyomas, we used global expression pro ... Full text Link to item Cite

The changing face of peer review at the National Institutes of Health.

Journal article Fertil Steril · February 2004 Featured Publication OBJECTIVE: To discuss the new peer review system at the National Institutes of Health (NIH) and to present the process undertaken to accomplish these changes, with an emphasis on the reproductive sciences. DESIGN: A discussion of the many NIH committees an ... Full text Link to item Cite

Strategy for elucidating differentially expressed genes in leiomyomata identified by microarray technology.

Journal article Fertil Steril · August 2003 Featured Publication OBJECTIVE: cDNA microarray technology identifies genes that are differentially expressed between tissues. Our previous study identified several genes that might contribute to the fibroid phenotype. We therefore sought to confirm genes involved in three dis ... Full text Link to item Cite

Overview of women's health.

Journal article Clin Obstet Gynecol · December 2002 Featured Publication Full text Link to item Cite

Providing research and research training infrastructures for clinical research in the reproductive sciences.

Journal article Am J Obstet Gynecol · October 2002 Featured Publication The Reproductive Sciences Branch of the National Institutes of Child Health and Human Development has created pioneering new research and research training programs that address the impending shortage of physician-scientists in obstetrics, gynecology, and ... Full text Link to item Cite

Ascorbic acid status in postmenopausal women with hormone replacement therapy.

Journal article Maturitas · January 30, 2002 Featured Publication OBJECTIVES: Hormone oral contraceptives affected ascorbic acid status adversely in young women. In vitro, estrogens and progesterone inhibited ascorbic acid accumulation in intestinal cells. This is a pilot study to examine the relation between hormone rep ... Full text Link to item Cite

A survey of past obstetrics and gynecology research fellows.

Journal article J Soc Gynecol Investig · 2002 Featured Publication OBJECTIVE: To survey obstetrician-gynecologist-scientists to determine factors that enhanced or hindered research careers. METHODS: A pretested mail survey was sent to 107 obstetrician-gynecologists who had received research fellowship awards from professi ... Link to item Cite

Further evidence of a decorin-collagen interaction in the disruption of cervical collagen fibers during rat gestation.

Journal article Am J Obstet Gynecol · April 2000 Featured Publication OBJECTIVE: Our purpose was to determine the histologic distribution and expression of decorin in rat cervix throughout the course of rat gestation and to correlate the findings with the known progression of collagen fiber disruption and cellular turnover i ... Full text Link to item Cite

Proliferation and apoptosis of fibroblasts and smooth muscle cells in rat uterine cervix throughout gestation and the effect of the antiprogesterone onapristone.

Journal article Am J Obstet Gynecol · April 1998 Featured Publication OBJECTIVE: The purpose of this study was to determine the relative percentages of apoptosis and proliferation in fibroblasts and cervical smooth muscles throughout gestation and the effect of an antiprogesterone on these processes. STUDY DESIGN: Rats were ... Full text Link to item Cite

Reaction of residents to a teaching collaborative practice.

Journal article J Nurse Midwifery · 1998 Featured Publication Using the team concept, certified nurse-midwives, nurse practitioners, and physician assistants help educate physicians in an obstetrics and gynecology residency program and help to create a balance between education and service. This program is well recei ... Full text Link to item Cite

Cell death and proliferation and its relation to collagen degradation in uterine involution of rat.

Journal article Connect Tissue Res · 1998 Featured Publication Collagen concentration, procollagenase localization, and their association with cell proliferation and apoptosis during postpartum involution, were investigated biochemically and histochemically in postpartum day 1, 3, 5, and 7 rat uterine tissues. In cont ... Full text Link to item Cite

Medicaid managed care partnerships to improve perinatal outcomes.

Journal article J Public Health Manag Pract · January 1998 Featured Publication New York State's Prenatal Care Assistance Program, and enhanced care program based on public health principles, is in the process of being transformed into Medicaid managed care. The program described in this article, namely, a Medicaid managed care health ... Full text Link to item Cite

Residents and nonphysician health care providers.

Journal article Acad Med · March 1997 Featured Publication Full text Link to item Cite

The challenges and opportunities of collaborative practice.

Journal article Womens Health Issues · 1997 Featured Publication Full text Link to item Cite

Analyzing strategies for developing a prenatal health care outreach program to reduce social and cultural barriers.

Journal article J Health Hum Serv Adm · 1997 Featured Publication Beginning in March, 1994, a multi-cultural, interdisciplinary team of health care providers at Rochester General Hospital in Rochester, New York, planned and implemented a prenatal outreach program in partnership with the Rochester YWCA. The purpose of the ... Link to item Cite

Teaching sensitivity to cultural issues in women's health care in a community hospital setting.

Journal article Am J Prev Med · 1996 Featured Publication Link to item Cite

Learning from the community about barriers to health care.

Journal article Obstet Gynecol · January 1996 Featured Publication One of the major challenges facing obstetrician-gynecologists, especially those serving populations that are diverse in culture and circumstances, is to identify and address the barriers that keep women from seeking timely preventive and prenatal health ca ... Full text Link to item Cite

Collagen changes in rat cervix in pregnancy--polarized light microscopic and electron microscopic studies.

Journal article Proc Soc Exp Biol Med · September 1995 Featured Publication The structural arrangement of collagen fibers in cervical ripening was studied in normal pregnant rats by picrosirius red staining and polarized light microscopy. The macromolecular arrangement of collagen fibers in the cervices of nonpregnant controls and ... Full text Link to item Cite

Anatomy and physiology of cervical ripening.

Journal article Clin Obstet Gynecol · June 1995 Featured Publication The uterine cervix is a unique organ composed predominately of the extracellular matrix proteins, collagen, elastin, and glycosaminoglycans. During pregnancy and labor, this organ is metabolically active, which is rare in adult tissue. The metabolism is un ... Full text Link to item Cite

Apoptosis in the cervix of pregnant rats in association with cervical softening.

Journal article Gynecol Obstet Invest · 1994 Featured Publication Cervical ripening in gestation occurs spontaneously in a timely but species-specific fashion as if it were genetically programmed. This study shows that the numbers of dying smooth muscle cells in the cervix increased along with cervical softening in pregn ... Full text Link to item Cite

Effect of parturition on serum alpha 1-antiprotease (alpha 1-antitrypsin).

Journal article Clin Chem · May 1993 Featured Publication Link to item Cite

An analysis of the reasons for Japan's low infant mortality rate.

Journal article J Nurse Midwifery · 1993 Featured Publication Japan's infant mortality rate in 1991 was four per 1,000, the lowest in the world. Contributing factors are the universal use of the Boshi Kenko Techo (maternal-child health handbook) and universal access to care. Most births occur to women aged 25-29 year ... Full text Link to item Cite

Three-dimensional structures of uterine elastic fibers: scanning electron microscopic studies.

Journal article Connect Tissue Res · 1991 Featured Publication We report findings that demonstrate for the first time that the structure of the elastic fibers of the uterus and cervix are characteristic to these tissues. Elastic fibers of the uterine corpus and cervix were studied by scanning electron microscopy (SEM) ... Full text Link to item Cite

Obstetric-enhanced family practice residency training.

Journal article J Fam Pract · November 1990 Featured Publication Link to item Cite

Effects of epidural analgesia: some questions and answers.

Journal article Birth · September 1990 Featured Publication The effects of epidural analgesia on first labors have been studied by Thorp and colleagues. One study has been published and is the subject of a question-and-answer discussion, presented here. In this study 711 consecutive nulliparous women at term, with ... Full text Link to item Cite

Human recombinant interleukin-1 alpha increases elastase-like enzyme in human uterine cervical fibroblasts.

Journal article Gynecol Obstet Invest · 1990 Featured Publication Production of the elastase-like enzyme, Suc-(Ala)3-p-nitroanilide hydrolase, was examined in human uterine cervical fibroblasts. The metallo-dependent enzyme is present in both media and cellular fractions. Human recombinant interleukin-1 alpha stimulated ... Full text Link to item Cite

Decreased elastic fibers and desmosine content in incompetent cervix.

Journal article Am J Obstet Gynecol · November 1987 Featured Publication Incompetence of the uterine cervix is a syndrome of painless, progressive dilatation and effacement occurring between the sixteenth and twenty-fourth weeks of gestation that represents abnormal functioning. It may serve as a model to elucidate normal funct ... Full text Link to item Cite

Orientation of elastic fibers in the human cervix.

Journal article Am J Obstet Gynecol · July 1986 Featured Publication We have determined the microanatomy of the cervix in relation to elastic fibers by serial dissection of human cervix in three tissue planes: cross, sagittal, and frontal sections. Analysis suggests that elastin is localized to specific regions of the uteri ... Full text Link to item Cite

Neonatal alloimmune thrombocytopenic purpura.

Journal article Am J Perinatol · April 1986 Featured Publication We reviewed 58 literature reports of neonatal alloimmune thrombocytopenic purpura (NAITP). The mortality rate was 9%. The total incidence of suspected intracranial hemorrhage was 28%. We reviewed 17 sibship cases for the relation of birth order to treatmen ... Full text Link to item Cite

Pregnancy outcomes among adolescent and older women receiving comprehensive prenatal care.

Journal article J Adolesc Health Care · March 1986 Featured Publication This study compares three birth outcomes, gestational age, birthweight, and type of delivery, among adolescent and older mothers. Data were collected from 911 women who attended a comprehensive prenatal-care program in a large urban teaching hospital. Wome ... Full text Link to item Cite

Neonatal alloimmune thrombocytopenic purpura: a case report.

Journal article Am J Obstet Gynecol · January 1986 Featured Publication We report a case of a sibling pair with neonatal alloimmune thrombocytopenic purpura. Serial antepartum platelet alloantibody quantitation by an enzyme-linked immunoabsorbent assay revealed rising antibody titers during advancing gestation. We discuss the ... Full text Link to item Cite

Cesarean section deliveries among adolescent mothers enrolled in a comprehensive prenatal care program.

Journal article Am J Obstet Gynecol · July 15, 1985 Featured Publication A comparison was made of the incidences of cesarean section deliveries between mothers 18 years of age or younger and those 19 to 21 years of age, all of whom were enrolled in a comprehensive program of prenatal care. Also compared were the indications for ... Full text Link to item Cite

The tendency to IUGR in preterm infants born to young women enrolled in a comprehensive prenatal program.

Journal article Am J Perinatol · April 1985 Featured Publication Low-birthweight infants born to young women in an urban poor and multiethnic community who attended an intensive comprehensive prenatal program were studied. Of the 801 young women followed, 69 (8.6%) delivered infants weighing less than or equal to 2500 g ... Full text Link to item Cite

Nutrition for the diabetic pregnant woman.

Journal article Curr Concepts Nutr · 1985 Featured Publication Link to item Cite

Cost averted by providing comprehensive prenatal care to teenagers.

Journal article J Nurse Midwifery · 1985 Featured Publication Full text Link to item Cite

Spontaneous regression of fetal ascites in utero in an adolescent.

Journal article J Adolesc Health Care · October 1984 Featured Publication Fetal ascites has been documented by ultrasound to progressively disappear prior to birth. This report describes a case in a nineteen-year-old in which fetal ascites was documented in the midtrimester of pregnancy and in which subsequent regression was sho ... Full text Link to item Cite

Grieving characteristics after spontaneous abortion: a management approach.

Journal article Obstet Gynecol · July 1984 Featured Publication Twenty-two women who experienced a spontaneous abortion were treated during a 15-month period. All patients were seen for counseling sessions immediately after the spontaneous abortion. The initial counseling was followed by patient contacts and by a secon ... Link to item Cite

The effect of pregnancy on adolescent growth and development.

Journal article Women Health · 1984 Featured Publication Adolescent pregnancy represents a triple developmental crisis to young women, that of adolescence, pregnancy, and establishing a relationship with a member of the opposite sex. Physiologically the adjustments of pregnancy are superimposed on those of pubes ... Full text Link to item Cite

The content of elastin in the uterine cervix.

Journal article Arch Biochem Biophys · April 1, 1983 Featured Publication Mature, crosslinked elastin has been isolated from 4 human and 12 monkey uterine cervices. A modification of previous methods for determination of elastin content was devised to quantitate the low amounts of elastin in the crude connective tissue of uterin ... Full text Link to item Cite

Conclusive evidence for the presence of elastin in human and monkey cervix.

Journal article Am J Obstet Gynecol · January 15, 1982 Featured Publication Full text Link to item Cite

Hyaline membrane disease in twin B following prolonged rupture of membranes for twin A.

Journal article Conn Med · February 1981 Featured Publication Link to item Cite

Fetus papyraceus causing dystocia: inability to detect blighted twin antenatally.

Journal article Obstet Gynecol · September 1979 Featured Publication A case is reported of fetus papyraceus diagnosed intrapartum. There was no clinical evidence in early and midpregnancy to suggest such a diagnosis. Ultrasound examination performed 1 week prior to delivery was nondiagnostic, but an x-ray taken intrapartum ... Link to item Cite