Trey Highland

Journal article Biomedical Optics Express · July 1, 2026 Spectroscopic optical coherence tomography (SOCT) allows for the targeted analysis of cell nuclei due to their spectrally distinct scattering properties. SOCT has been explored for optical biopsy based on its ability to extract structural and spectroscopic ... Full text Cite

Real-time processing of high-throughput quantitative phase microscopy data using a Jetson Orin Nano.

Journal article Biophotonics discovery · January 2026 SignificanceQuantitative phase microscopy (QPM) is a holographic imaging technique often applied to studying cell morphology. To advance QPM for clinical applications, high-throughput implementations have been developed to allow imaging of thousan ... Full text Cite

Spectroscopic analysis of volumetric OCT data for the automated measurement of scatterer size.

Journal article Journal of the Optical Society of America. A, Optics, image science, and vision · September 2025 Spectroscopic optical coherence tomography enables an accurate estimation of scatterer size by computing the correlation distance (CD) function. For calibration and accuracy verification, polystyrene spheres are commonly used as size standards. However, an ... Full text Cite

Enhanced penetration depth in optical coherence tomography and photoacoustic microscopy <i>in vivo</i> enabled by absorbing dye molecules.

Journal article Optica · January 2025 The scattering and absorption of light within biological tissue severely limits the penetration depth of optical imaging techniques. Recently, it has been found that water-soluble, strongly absorbing dye molecules, such as tartrazine, can achieve in viv ... Full text Cite

Pressure Monitoring Model for Investigating the Response of Mouse Optic Nerve Head Astrocyte to Elevated Hydrostatic Pressures

Conference Clinical and Translational Biophotonics Translational 2025 in Proceedings Optica Biophotonics Congress 2025 Part of Optica Biophotonics Congress Optics in the Life Sciences · January 1, 2025 We designed a system to adjust and monitoring pressure to simulate intraocular pressure associated with glaucoma. Using quantitative phase microscopy and Förster resonance energy transfer, we compared cellular responses under normal and elevated pressure c ... Full text Cite

Multimodal segmentation of dynamic subcellular features using quantitative phase imaging and FRET-based sensors [Invited].

Journal article Journal of the Optical Society of America. A, Optics, image science, and vision · November 2024 Understanding cellular responses to mechanical environmental stimuli is important for cellular mechanotransduction studies. While fluorescence microscopy has been used for aiding mechanotransduction research due to its molecular sensitivity, the ability of ... Full text Cite

Utilizing quantitative phase microscopy to localize fluorescence in three dimensions via the transport of intensity equation.

Journal article Optics letters · August 2024 We demonstrate the use of a novel, to the best of our knowledge, localization algorithm for digitally refocusing fluorescence images from a three-dimensional cell culture. Simultaneous phase and fluorescence intensity images are collected through a multimo ... Full text Cite