Satish K. Chitneni | Scholars@Duke

Satish K. Chitneni
Associate Professor in Radiology

The major focus of my research is on the design, development and evaluation of novel radiotracers based on small molecules for imaging of specific molecular targets by positron emission tomography (PET). The imaging targets are usually enzymes, cell surface receptors or transporters that are strongly implicated in or markers of diseases. Fluorine-18, which has a half-life of about 110 min, is ideally suited for radiolabeling of small molecules, and permits PET imaging studies for up to 4 h after injection in vivo. Other radioisotopes of interest for PET radiotracer development include carbon-11 and iodine-124, the latter one has a longer half-life (4.2 days) and enables imaging at late time points. Another major area of interest is the use of PET imaging in conjunction with suitable radiotracers for evaluation of novel therapeutics in preclinical studies.

Utilizing the wealth of information available from the cancer genome sequencing studies and The Cancer Genome Atlas (TCGA) studies, efforts are underway to develop radiolabeled probes for PET imaging of isocitrate dehydrogenase 1 (IDH1) mutations, which are present in the vast majority of patients with lower-grade gliomas and secondary higher-grade gliomas. Successful development of imaging methods for mutant IDH1 and other important genetic alterations in gliomas can help in molecular and genetic classification of gliomas noninvasively and in developing novel therapeutics.

Current Appointments & Affiliations

Associate Professor in Radiology, Radiology, Clinical Science Departments 2019
Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 2013
Faculty Network Member of the Duke Institute for Brain Sciences, Duke Institute for Brain Sciences, University Institutes and Centers 2017

Contact Information

311 Research Drive, 161-I Bryan Research Building, Durham, NC 27710
Box 3949 Med Ctr, Durham, NC 27710
satish.chitneni@duke.edu (919) 684-7809

Background
Education, Training, & Certifications
- Ph.D., Katholieke Universiteit Leuven (Belgium) 2007
Duke Appointment History
- Assistant Professor of Radiology, Radiology, Clinical Science Departments 2018 - 2019
- Assistant Professor of Radiology, Radiology, Clinical Science Departments 2011 - 2018
Expertise
Subject Headings
Research
Selected Grants
- Labeling nanobodies with 18F residualizing labels for HER2 specific PET imaging awarded by National Institutes of Health 2015 - 2020
- EGFRvIII-targeted Bispecific T cell Engagers for brain tumors awarded by National Institutes of Health 2013 - 2019
- Novel Approach to Quantify Nicotinic Receptor Upregulation in Smokers awarded by National Institutes of Health 2014 - 2017
- Imaging IDH1 Mutations in Glioma and Other Malignancies awarded by National Institutes of Health 2014 - 2016
Publications & Artistic Works
Selected Publications
- Academic Articles
  - Du, K., Chitneni, S. K., Suzuki, A., Wang, Y., Henao, R., Hyun, J., … Diehl, A. M. (2019). Increased Glutaminolysis Marks Active Scarring in Nonalcoholic Steatohepatitis Progression. Cell Mol Gastroenterol Hepatol. https://doi.org/10.1016/j.jcmgh.2019.12.006
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  - Chitneni, S. K., Koumarianou, E., Vaidyanathan, G., & Zalutsky, M. R. (2019). Observations on the Effects of Residualization and Dehalogenation on the Utility of N-Succinimidyl Ester Acylation Agents for Radioiodination of the Internalizing Antibody Trastuzumab. Molecules, 24(21). https://doi.org/10.3390/molecules24213907
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  - Gedeon, P. C., Schaller, T. H., Chitneni, S. K., Choi, B. D., Kuan, C.-T., Suryadevara, C. M., … Sampson, J. H. (2018). A Rationally Designed Fully Human EGFRvIII:CD3-Targeted Bispecific Antibody Redirects Human T Cells to Treat Patient-derived Intracerebral Malignant Glioma. Clin Cancer Res, 24(15), 3611–3631. https://doi.org/10.1158/1078-0432.CCR-17-0126
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  - Chitneni, S. K., Yan, H., & Zalutsky, M. R. (2018). Synthesis and Evaluation of a 18F-Labeled Triazinediamine Analogue for Imaging Mutant IDH1 Expression in Gliomas by PET. Acs Med Chem Lett, 9(7), 606–611. https://doi.org/10.1021/acsmedchemlett.7b00478
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  - Zhou, Z., Chitneni, S. K., Devoogdt, N., Zalutsky, M. R., & Vaidyanathan, G. (2018). Fluorine-18 labeling of an anti-HER2 VHH using a residualizing prosthetic group via a strain-promoted click reaction: Chemistry and preliminary evaluation. Bioorg Med Chem, 26(8), 1939–1949. https://doi.org/10.1016/j.bmc.2018.02.040
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  - Chitneni, S. K., Reitman, Z. J., Spicehandler, R., Gooden, D. M., Yan, H., & Zalutsky, M. R. (2018). Synthesis and evaluation of radiolabeled AGI-5198 analogues as candidate radiotracers for imaging mutant IDH1 expression in tumors. Bioorg Med Chem Lett, 28(4), 694–699. https://doi.org/10.1016/j.bmcl.2018.01.015
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  - Chitneni, S. K., Reitman, Z. J., Gooden, D. M., Yan, H., & Zalutsky, M. R. (2016). Radiolabeled inhibitors as probes for imaging mutant IDH1 expression in gliomas: Synthesis and preliminary evaluation of labeled butyl-phenyl sulfonamide analogs. Eur J Med Chem, 119, 218–230. https://doi.org/10.1016/j.ejmech.2016.04.066
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  - Chitneni, S. K. (2016). IDH1 Mutations in Glioma: Considerations for Radiotracer Development. Sm Radiol J, 2(1).
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  - Chitneni, S. K., Bida, G. T., Zalutsky, M. R., & Dewhirst, M. W. (2015). Reply: Pharmacokinetic and Pharmacodynamic Modifiers of EF5 Uptake and Binding. J Nucl Med, 56(4), 653–654. https://doi.org/10.2967/jnumed.115.154054
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  - Pruszynski, M., Koumarianou, E., Vaidyanathan, G., Chitneni, S., & Zalutsky, M. R. (2015). D-Amino acid peptide residualizing agents bearing N-hydroxysuccinimido- and maleimido-functional groups and their application for trastuzumab radioiodination. Nucl Med Biol, 42(1), 19–27. https://doi.org/10.1016/j.nucmedbio.2014.08.007
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  - Safi, R., Nelson, E. R., Chitneni, S. K., Franz, K. J., George, D. J., Zalutsky, M. R., & McDonnell, D. P. (2014). Copper signaling axis as a target for prostate cancer therapeutics. Cancer Res, 74(20), 5819–5831. https://doi.org/10.1158/0008-5472.CAN-13-3527
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  - Chitneni, S. K., Bida, G. T., Zalutsky, M. R., & Dewhirst, M. W. (2014). Comparison of the Hypoxia PET Tracer (18)F-EF5 to Immunohistochemical Marker EF5 in 3 Different Human Tumor Xenograft Models. J Nucl Med, 55(7), 1192–1197. https://doi.org/10.2967/jnumed.114.137448
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  - Akurathi, V., Dubois, L., Celen, S., Lieuwes, N. G., Chitneni, S. K., Cleynhens, B. J., … Bormans, G. M. (2014). Development and biological evaluation of ⁹⁹mTc-sulfonamide derivatives for in vivo visualization of CA IX as surrogate tumor hypoxia markers. Eur J Med Chem, 71, 374–384. https://doi.org/10.1016/j.ejmech.2013.10.027
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  - Chitneni, S. K., Bida, G. T., Yuan, H., Palmer, G. M., Hay, M. P., Melcher, T., … Dewhirst, M. W. (2013). 18F-EF5 PET imaging as an early response biomarker for the hypoxia-activated prodrug SN30000 combined with radiation treatment in a non-small cell lung cancer xenograft model. J Nucl Med, 54(8), 1339–1346. https://doi.org/10.2967/jnumed.112.116293
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  - Deroose, C. M., Chitneni, S. K., Gijsbers, R., Vermaelen, P., Ibrahimi, A., Balzarini, J., … Mortelmans, L. (2012). Preliminary validation of varicella zoster virus thymidine kinase as a novel reporter gene for PET. Nucl Med Biol, 39(8), 1266–1274. https://doi.org/10.1016/j.nucmedbio.2012.06.014
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  - Chitneni, S. K., Bida, G. T., Dewhirst, M. W., & Zalutsky, M. R. (2012). A simplified synthesis of the hypoxia imaging agent 2-(2-Nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-[(18)F]pentafluoropropyl)-acetamide ([18F]EF5). Nucl Med Biol, 39(7), 1012–1018. https://doi.org/10.1016/j.nucmedbio.2012.05.006
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  - Chitneni, S. K., Bida, G. T., Hay, M. P., Zalutsky, M. R., Melcher, T., Wilson, W. R., & Dewhirst, M. W. (2011). Abstract 5306:18F-EF5 microPET imaging of treatment response from a novel, hypoxia-selective cytotoxin SN30000 in a human lung cancer xenograft model. Tumor Biology. https://doi.org/10.1158/1538-7445.am2011-5306
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  - Chitneni, S. K., Palmer, G. M., Zalutsky, M. R., & Dewhirst, M. W. (2011). Molecular imaging of hypoxia. J Nucl Med, 52(2), 165–168. https://doi.org/10.2967/jnumed.110.075663
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  - Celen, S., Koole, M., De Angelis, M., Sannen, I., Chitneni, S. K., Alcazar, J., … Bormans, G. (2010). Preclinical evaluation of 18F-JNJ41510417 as a radioligand for PET imaging of phosphodiesterase-10A in the brain. J Nucl Med, 51(10), 1584–1591. https://doi.org/10.2967/jnumed.110.077040
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  - Akurathi, V., Dubois, L., Lieuwes, N. G., Chitneni, S. K., Cleynhens, B. J., Vullo, D., … Bormans, G. M. (2010). Synthesis and biological evaluation of a 99mTc-labelled sulfonamide conjugate for in vivo visualization of carbonic anhydrase IX expression in tumor hypoxia. Nucl Med Biol, 37(5), 557–564. https://doi.org/10.1016/j.nucmedbio.2010.02.006
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  - Akurathi, V., Dubois, L., Lieuwes, N. G., Chitneni, S. K., Cleynhens, B. J., Verbruggen, A. M., … Bormans, G. (2009). Synthesis and Biological Evaluation of 99mTc-Labelled Aminoethylbenzenesulfonamide Conjugate for In vivo Visualisation of Carbonic Anhydrase IX (CAIX) Expression in Tumor Hypoxia. European Journal of Nuclear Medicine and Molecular Imaging, 36, S309–S309.
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  - Celen, S., De Angelis, M., Chitneni, S. K., Alcazar, J., Koole, M., Dedeurwaerdere, S., … Bormans, G. (2009). Synthesis and preliminary biological evaluation of [F-18]JNJ41510417as a radioligand for positron emission tomography imaging of phosphodiesterase-10A in the brain. European Journal of Nuclear Medicine and Molecular Imaging, 36, S236–S236.
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  - Akurathi, V., Chitneni, S., Cleynhens, B., Verbruggen, A., & Bormans, G. (2009). DEVELOPMENT AND PRELIMINARY EVALUATION OF A 11C LABELED SULFONAMIDE DERIVATES AS POTENTIAL PET TRACER FOR BLOOD POOL IMAGING. Journal of Labelled Compounds & Radiopharmaceuticals, 52, S341–S341.
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  - Akurathi, V., Dubois, L., Lieuwes, N., Chitneni, S. K., Cleynhens, B., Verbruggen, A., … Bormans, G. (2009). SYNTHESIS AND BIOLOGICAL EVALUATION OF A NEGATIVELY CHARGED 99mTc-LABELLED AMINOETHYLBENZENE SULFONAMIDE CONJUGATE FOR IN VIVO VISUALISATION OF CARBONIC ANHYDRASE IX (CA IX) EXPRESSION AS A SURROGATE MARKER OF TUMOR HYPOXIA. Journal of Labelled Compounds & Radiopharmaceuticals, 52, S135–S135.
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  - Chitneni, S. K., Serdons, K., Evens, N., Fonge, H., Celen, S., Deroose, C. M., … Bormans, G. M. (2008). Efficient purification and metabolite analysis of radiotracers using high-performance liquid chromatography and on-line solid-phase extraction. J Chromatogr A, 1189(1–2), 323–331. https://doi.org/10.1016/j.chroma.2007.10.084
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  - Chitneni, S. K., Balzarini, J., Celen, S., Dyubankova, N., Verbruggen, A. M., & Bormans, G. M. (2008). Synthesis and biological evaluation of carbon-11-labeled acyclic and furo[2,3-d]pyrimidine derivatives of bicyclic nucleoside analogues (BCNAs) for structure-brain uptake relationship study of BCNA tracers. Journal of Labelled Compounds and Radiopharmaceuticals, 51(3), 159–166. https://doi.org/10.1002/jlcr.1494
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  - Celen, S., Deroose, C., de Groot, T., Chitneni, S. K., Gijsbers, R., Debyser, Z., … Bormans, G. (2008). Synthesis and evaluation of 18F- and 11C-labeled phenyl-galactopyranosides as potential probes for in vivo visualization of LacZ gene expression using positron emission tomography. Bioconjug Chem, 19(2), 441–449. https://doi.org/10.1021/bc700216d
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  - Chitneni, S. K., Garreau, L., Cleynhens, B., Evens, N., Bex, M., Vermaelen, P., … Bormans, G. (2008). Improved synthesis and metabolic stability analysis of the dopamine transporter ligand [(18)F]FECT. Nucl Med Biol, 35(1), 75–82. https://doi.org/10.1016/j.nucmedbio.2007.09.001
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  - Chitneni, S. K., Deroose, C. M., Balzarini, J., Gijsbers, R., Celen, S., Debyser, Z., … Bormans, G. M. (2007). A p-[18F]fluoroethoxyphenyl bicyclic nucleoside analogue as a potential positron emission tomography imaging agent for varicella-zoster virus thymidine kinase gene expression. J Med Chem, 50(26), 6627–6637. https://doi.org/10.1021/jm700971p
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  - Fonge, H., Chitneni, S. K., Lixin, J., Vunckx, K., Prinsen, K., Nuyts, J., … Verbruggen, A. (2007). Necrosis avidity of (99m)Tc(CO)3-labeled pamoic acid derivatives: synthesis and preliminary biological evaluation in animal models of necrosis. Bioconjug Chem, 18(6), 1924–1934. https://doi.org/10.1021/bc700236j
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  - Chitneni, S. K., Deroose, C. M., Fonge, H., Gijsbers, R., Dyubankova, N., Balzarini, J., … Bormans, G. M. (2007). Synthesis and biological evaluation of an 123I-labeled bicyclic nucleoside analogue (BCNA) as potential SPECT tracer for VZV-tk reporter gene imaging. Bioorg Med Chem Lett, 17(12), 3458–3462. https://doi.org/10.1016/j.bmcl.2007.03.081
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  - Chitneni, S. K., De Ruymaeker, T., Balzarini, J., Verbruggen, A. M., & Bormans, G. M. (2007). Synthesis and biological evaluation of a lipophilic, fluorine-18-labeled 5-ethynyl-2′-deoxyuridine derivative. Journal of Labelled Compounds and Radiopharmaceuticals, 50(7), 649–655. https://doi.org/10.1002/jlcr.1312
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  - Toelen, J., Deroose, C. M., Gijsbers, R., Reumers, V., Sbragia, L. N., Vets, S., … Debyser, Z. (2007). Fetal gene transfer with lentiviral vectors: long-term in vivo follow-up evaluation in a rat model. Am J Obstet Gynecol, 196(4), 352.e1-352.e6. https://doi.org/10.1016/j.ajog.2007.01.038
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  - Chitneni, S. K., Deroose, C. M., Balzarini, J., Gijsbers, R., Celen, S. J. L., de Groot, T. J., … Bormans, G. M. (2007). Synthesis and preliminary evaluation of 18F- or 11C-labeled bicyclic nucleoside analogues as potential probes for imaging varicella-zoster virus thymidine kinase gene expression using positron emission tomography. J Med Chem, 50(5), 1041–1049. https://doi.org/10.1021/jm060964m
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  - Chitneni, S., Deroose, C., Balzarini, J., Verbruggen, A., & Bormans, G. (2006). Synthesis and preliminary evaluation of F-18 and C-11 labeled bi-cyclic nucleoside analogues (BCNAs) as potential PET reporter probes for imaging varicella-zoster virus thymidine kinase (VZV-tk) gene expression. European Journal of Nuclear Medicine and Molecular Imaging, 33, S175–S175.
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  - Chitneni, S., Cleynhens, B., de Groot, T., Verbruggen, A., & Bormans, G. (2005). A New Precursor for a High Yield Synthesis of[F-18]FHBG. European Journal of Nuclear Medicine and Molecular Imaging, 32, S268–S269.
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- Conference Papers
  - Chitneni, S., Reitman, Z., Yan, H., & Zalutsky, M. (2014). Radioiodinated AGI-5198 for in vivo imaging of isocitrate dehydrogenase 1 (IDH1) mutation in gliomas. In Journal of Nuclear Medicine (Vol. 55). St Louis, MO: SOC NUCLEAR MEDICINE INC.
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  - Chitneni, S., Bida, G., Dewhirst, M., & Zalutsky, M. (2011). A simplified synthesis of the hypoxia imaging agent 2-(2-Nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-[18F]pentafluoropropyl)-acetamide([18F]EF5). In Journal of Nuclear Medicine (Vol. 52). SOC NUCLEAR MEDICINE INC.
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  - Celen, S., Koole, M., De Angelis, M., Sannen, I., Chitneni, S. K., Alcazar, J., … Bormans, G. (2010). [18F]JNJ41510417 a potential PET radioligand for imaging phosphodiesterase-10A in the brain. In Neuroimage (Vol. 52, pp. S15–S15). Elsevier BV. https://doi.org/10.1016/j.neuroimage.2010.04.200
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  - Chitneni, S. K., Balzarini, J., Verbruggen, A., & Bormans, G. (2007). Synthesis and biological evaluation of C-11-labeled acyclic- and furo[2,3-d] pyrimidine derivative of bicyclic nucleoside analogs (BCNAs) for structure-brain uptake relationship study of BCNA tracers. In European Journal of Nuclear Medicine and Molecular Imaging (Vol. 34, pp. S331–S332). Copenhagen, DENMARK: SPRINGER.
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  - Kumar Chitneni, S., Govaerts, C., Adams, E., Van Schepdael, A., & Hoogmartens, J. (2004). Identification of impurities in erythromycin by liquid chromatography-mass spectrometric detection. In J Chromatogr A (Vol. 1056, pp. 111–120). Netherlands. https://doi.org/10.1016/j.chroma.2004.07.091
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Education, Training, & Certifications

Duke Appointment History

Subject Headings

Selected Grants

Selected Publications

Academic Articles

Conference Papers