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Sheila Collins

Adjunct Associate Professor in Psychiatry and Behavioral Sciences
Psychiatry & Behavioral Sciences, Adult Psychiatry & Psychology
6400 Sanger Road, Sanford-Burnham Medical Research Inst, Orlando, FL 32827
6400 Sanger Road, Sanford-Burnham Medical Research Inst, Orlando, FL 32827

Selected Publications


Brown adipose tissue and body weight regulation

Chapter · January 1, 2018 Brown adipose tissue plays a critical role in neonatal thermogenesis, insuring survival of the newborn infant during the transition from intrauterine to extrauterine life. In rodents, the generation and/or expansion of brown adipose tissue within white adi ... Full text Cite

Activation of mTORC1 is essential for β-adrenergic stimulation of adipose browning

Journal Article Journal of Clinical Investigation · March 28, 2016 Full text Open Access Cite

Nuclear factor erythroid-derived factor 2-related factor 2 regulates transcription of CCAAT/enhancer-binding protein β during adipogenesis.

Journal Article Free Radic Biol Med · January 15, 2012 Nuclear factor erythroid-derived factor 2-related factor 2 (Nrf2) is a cap-n-collar basic leucine zipper transcription factor that is involved in the cellular adaptive response to oxidative stress. Our previous study reported that targeted disruption of th ... Full text Link to item Cite

ROS signaling, oxidative stress and Nrf2 in pancreatic beta-cell function.

Journal Article Toxicol Appl Pharmacol · April 1, 2010 This review focuses on the emerging evidence that reactive oxygen species (ROS) derived from glucose metabolism, such as H(2)O(2), act as metabolic signaling molecules for glucose-stimulated insulin secretion (GSIS) in pancreatic beta-cells. Particular emp ... Full text Link to item Cite

Persistent oxidative stress due to absence of uncoupling protein 2 associated with impaired pancreatic beta-cell function.

Journal Article Endocrinology · July 2009 Uncoupling protein (UCP) 2 is a widely expressed mitochondrial protein whose precise function is still unclear but has been linked to mitochondria-derived reactive oxygen species production. Thus, the chronic absence of UCP2 has the potential to promote pe ... Full text Link to item Cite

Liver X receptor is a regulator of orphan nuclear receptor NOR-1 gene transcription in adipocytes.

Journal Article Int J Obes (Lond) · May 2009 OBJECTIVE: The liver X receptors (LXRs) are ligand-activated nuclear transcription factors that have been shown to play major roles in lipid, glucose and cholesterol metabolism. Recently, members of the NR4A orphan nuclear receptor family have also been sh ... Full text Link to item Cite

Reduced antioxidant capacity and diet-induced atherosclerosis in uncoupling protein-2-deficient mice.

Journal Article J Lipid Res · January 2009 Vascular dysfunction in response to reactive oxygen species (ROS) plays an important role in the development and progression of atherosclerotic lesions. In most cells, mitochondria are the major source of cellular ROS during aerobic respiration. Under most ... Full text Link to item Cite

Suppression of hepatic glucose production by human neutrophil alpha-defensins through a signaling pathway distinct from insulin.

Journal Article J Biol Chem · May 2, 2008 In this study, we tested the hypothesis that human neutrophil alpha-defensins (HNPs) inhibit hepatic glucose production through a signaling pathway distinct from insulin. The effect of HNP-1 on fasting blood glucose levels and the expression of hepatic glu ... Full text Link to item Cite

Liver X receptor alpha is a transcriptional repressor of the uncoupling protein 1 gene and the brown fat phenotype.

Journal Article Mol Cell Biol · April 2008 The adipocyte integrates crucial information about metabolic needs in order to balance energy intake, storage, and expenditure. Whereas white adipose tissue stores energy, brown adipose tissue is a major site of energy dissipation through adaptive thermoge ... Full text Link to item Cite

Activation of Nrf2-mediated oxidative stress response in macrophages by hypochlorous acid.

Journal Article Toxicol Appl Pharmacol · February 1, 2008 Hypochlorous acid (HOCl), a potent oxidant generated when chlorine gas reacts with water, is important in the pathogenesis of many disorders. Transcription factor Nrf2-mediated antioxidant response represents a critical cellular defense mechanism that serv ... Full text Link to item Cite

Requirement of vimentin filament assembly for beta3-adrenergic receptor activation of ERK MAP kinase and lipolysis.

Journal Article J Biol Chem · March 23, 2007 Catecholamine stimulation of beta-adrenergic receptors (betaAR) in adipocytes activates the cAMP-dependent protein kinase to promote liberation of fatty acids as a fuel source. The adipocyte beta3AR also activates extracellular signal-regulated kinases (ER ... Full text Link to item Cite

Maximal beta3-adrenergic regulation of lipolysis involves Src and epidermal growth factor receptor-dependent ERK1/2 activation.

Journal Article J Biol Chem · December 8, 2006 Catecholamine-stimulated lipolysis is primarily a beta-adrenergic and cAMP-dependent event. In previous studies we established that the beta(3)-adrenergic receptor (beta(3)AR) in adipocytes utilizes a unique mechanism to stimulate extracellular signal-regu ... Full text Link to item Cite

Adipose tissue development and metabolism

Chapter · December 1, 2006 Excess body fat is associated with many medical complications and obesity has become a major health problem in the United States and most other industrialized countries. The existence of adipose tissue as a specialized organ reflects the mechanism develope ... Full text Cite

UCP-2 limits the development of angiotensin II-induced renal sclerosis

Conference NEPHROLOGY DIALYSIS TRANSPLANTATION · July 1, 2006 Link to item Cite

p38 Mitogen-activated protein kinase plays a stimulatory role in hepatic gluconeogenesis.

Journal Article J Biol Chem · December 30, 2005 Hepatic gluconeogenesis is essential for maintaining blood glucose levels during fasting and is the major contributor to postprandial and fasting hyperglycemia in diabetes. Gluconeogenesis is a classic cAMP/protein kinase A-dependent process initiated by g ... Full text Link to item Cite

Overview of clinical perspectives and mechanisms of obesity.

Journal Article Birth Defects Res A Clin Mol Teratol · July 2005 Full text Link to item Cite

Selective activation of mitogen-activated protein (MAP) kinase kinase 3 and p38alpha MAP kinase is essential for cyclic AMP-dependent UCP1 expression in adipocytes.

Journal Article Mol Cell Biol · July 2005 The sympathetic nervous system regulates the activity and expression of uncoupling protein 1 (UCP1) through the three beta-adrenergic receptor subtypes and their ability to raise intracellular cyclic AMP (cAMP) levels. Unexpectedly, we recently discovered ... Full text Link to item Cite

Brown adipose tissue and body weight regulation

Chapter · January 1, 2018 Brown adipose tissue plays a critical role in neonatal thermogenesis, insuring survival of the newborn infant during the transition from intrauterine to extrauterine life. In rodents, the generation and/or expansion of brown adipose tissue within white adi ... Full text Cite

Activation of mTORC1 is essential for β-adrenergic stimulation of adipose browning

Journal Article Journal of Clinical Investigation · March 28, 2016 Full text Open Access Cite

Nuclear factor erythroid-derived factor 2-related factor 2 regulates transcription of CCAAT/enhancer-binding protein β during adipogenesis.

Journal Article Free Radic Biol Med · January 15, 2012 Nuclear factor erythroid-derived factor 2-related factor 2 (Nrf2) is a cap-n-collar basic leucine zipper transcription factor that is involved in the cellular adaptive response to oxidative stress. Our previous study reported that targeted disruption of th ... Full text Link to item Cite

ROS signaling, oxidative stress and Nrf2 in pancreatic beta-cell function.

Journal Article Toxicol Appl Pharmacol · April 1, 2010 This review focuses on the emerging evidence that reactive oxygen species (ROS) derived from glucose metabolism, such as H(2)O(2), act as metabolic signaling molecules for glucose-stimulated insulin secretion (GSIS) in pancreatic beta-cells. Particular emp ... Full text Link to item Cite

Persistent oxidative stress due to absence of uncoupling protein 2 associated with impaired pancreatic beta-cell function.

Journal Article Endocrinology · July 2009 Uncoupling protein (UCP) 2 is a widely expressed mitochondrial protein whose precise function is still unclear but has been linked to mitochondria-derived reactive oxygen species production. Thus, the chronic absence of UCP2 has the potential to promote pe ... Full text Link to item Cite

Liver X receptor is a regulator of orphan nuclear receptor NOR-1 gene transcription in adipocytes.

Journal Article Int J Obes (Lond) · May 2009 OBJECTIVE: The liver X receptors (LXRs) are ligand-activated nuclear transcription factors that have been shown to play major roles in lipid, glucose and cholesterol metabolism. Recently, members of the NR4A orphan nuclear receptor family have also been sh ... Full text Link to item Cite

Reduced antioxidant capacity and diet-induced atherosclerosis in uncoupling protein-2-deficient mice.

Journal Article J Lipid Res · January 2009 Vascular dysfunction in response to reactive oxygen species (ROS) plays an important role in the development and progression of atherosclerotic lesions. In most cells, mitochondria are the major source of cellular ROS during aerobic respiration. Under most ... Full text Link to item Cite

Suppression of hepatic glucose production by human neutrophil alpha-defensins through a signaling pathway distinct from insulin.

Journal Article J Biol Chem · May 2, 2008 In this study, we tested the hypothesis that human neutrophil alpha-defensins (HNPs) inhibit hepatic glucose production through a signaling pathway distinct from insulin. The effect of HNP-1 on fasting blood glucose levels and the expression of hepatic glu ... Full text Link to item Cite

Liver X receptor alpha is a transcriptional repressor of the uncoupling protein 1 gene and the brown fat phenotype.

Journal Article Mol Cell Biol · April 2008 The adipocyte integrates crucial information about metabolic needs in order to balance energy intake, storage, and expenditure. Whereas white adipose tissue stores energy, brown adipose tissue is a major site of energy dissipation through adaptive thermoge ... Full text Link to item Cite

Activation of Nrf2-mediated oxidative stress response in macrophages by hypochlorous acid.

Journal Article Toxicol Appl Pharmacol · February 1, 2008 Hypochlorous acid (HOCl), a potent oxidant generated when chlorine gas reacts with water, is important in the pathogenesis of many disorders. Transcription factor Nrf2-mediated antioxidant response represents a critical cellular defense mechanism that serv ... Full text Link to item Cite

Requirement of vimentin filament assembly for beta3-adrenergic receptor activation of ERK MAP kinase and lipolysis.

Journal Article J Biol Chem · March 23, 2007 Catecholamine stimulation of beta-adrenergic receptors (betaAR) in adipocytes activates the cAMP-dependent protein kinase to promote liberation of fatty acids as a fuel source. The adipocyte beta3AR also activates extracellular signal-regulated kinases (ER ... Full text Link to item Cite

Maximal beta3-adrenergic regulation of lipolysis involves Src and epidermal growth factor receptor-dependent ERK1/2 activation.

Journal Article J Biol Chem · December 8, 2006 Catecholamine-stimulated lipolysis is primarily a beta-adrenergic and cAMP-dependent event. In previous studies we established that the beta(3)-adrenergic receptor (beta(3)AR) in adipocytes utilizes a unique mechanism to stimulate extracellular signal-regu ... Full text Link to item Cite

Adipose tissue development and metabolism

Chapter · December 1, 2006 Excess body fat is associated with many medical complications and obesity has become a major health problem in the United States and most other industrialized countries. The existence of adipose tissue as a specialized organ reflects the mechanism develope ... Full text Cite

UCP-2 limits the development of angiotensin II-induced renal sclerosis

Conference NEPHROLOGY DIALYSIS TRANSPLANTATION · July 1, 2006 Link to item Cite

p38 Mitogen-activated protein kinase plays a stimulatory role in hepatic gluconeogenesis.

Journal Article J Biol Chem · December 30, 2005 Hepatic gluconeogenesis is essential for maintaining blood glucose levels during fasting and is the major contributor to postprandial and fasting hyperglycemia in diabetes. Gluconeogenesis is a classic cAMP/protein kinase A-dependent process initiated by g ... Full text Link to item Cite

Overview of clinical perspectives and mechanisms of obesity.

Journal Article Birth Defects Res A Clin Mol Teratol · July 2005 Full text Link to item Cite

Selective activation of mitogen-activated protein (MAP) kinase kinase 3 and p38alpha MAP kinase is essential for cyclic AMP-dependent UCP1 expression in adipocytes.

Journal Article Mol Cell Biol · July 2005 The sympathetic nervous system regulates the activity and expression of uncoupling protein 1 (UCP1) through the three beta-adrenergic receptor subtypes and their ability to raise intracellular cyclic AMP (cAMP) levels. Unexpectedly, we recently discovered ... Full text Link to item Cite

Persistent nuclear factor-kappa B activation in Ucp2-/- mice leads to enhanced nitric oxide and inflammatory cytokine production.

Journal Article J Biol Chem · May 13, 2005 One of the phenotypes of mice with targeted disruption of the uncoupling protein-2 gene (Ucp2-/-) is greater macrophage phagocytic activity and free radical production, resulting in a striking resistance to infectious microorganisms. In this study, the mol ... Full text Link to item Cite

Learning new tricks from old dogs: beta-adrenergic receptors teach new lessons on firing up adipose tissue metabolism.

Journal Article Mol Endocrinol · September 2004 The three beta AR (beta-adrenergic receptor) subtypes (beta(1)AR, beta(2)AR, and beta(3)AR) are members of the large family of G protein-coupled receptors, each of which is coupled to G alpha s and increases in intracellular cAMP levels. In white adipose t ... Full text Link to item Cite

Resistance to cerebral ischemic injury in UCP2 knockout mice: evidence for a role of UCP2 as a regulator of mitochondrial glutathione levels.

Journal Article J Neurochem · June 2004 Uncoupling protein 2 (UCP2) is suggested to be a regulator of reactive oxygen species production in mitochondria. We performed a detailed study of brain injury, including regional and cellular distribution of UCP2 mRNA, as well as measures of oxidative str ... Full text Link to item Cite

p38 mitogen-activated protein kinase is the central regulator of cyclic AMP-dependent transcription of the brown fat uncoupling protein 1 gene.

Journal Article Mol Cell Biol · April 2004 It is well established that catecholamine-stimulated thermogenesis in brown fat requires beta-adrenergic elevations in cyclic AMP (cAMP) to increase expression of the uncoupling protein 1 (UCP1) gene. However, little is known about the downstream component ... Full text Link to item Cite

Genetic vulnerability to diet-induced obesity in the C57BL/6J mouse: physiological and molecular characteristics.

Journal Article Physiol Behav · April 2004 The development of the metabolic syndrome in an increasing percentage of the populations of Western societies, particularly in the United States, requires valid models for establishing basic biochemical changes and performing preclinical studies on potenti ... Full text Link to item Cite

β-Adrenergic Receptors and Regulation of Energy Expenditure: A Family Affair

Book · March 10, 2004 The family of adrenergic receptors (ARs) expressed in adipocytes includes three sibling β ARs and two α AR cousins. Together they profoundly influence the mobilization of stored fatty acids, secretion of fat-cell derived hormones, and the specialized proce ... Full text Cite

Beta-adrenergic receptors and regulation of energy expenditure: a family affair.

Journal Article Annu Rev Pharmacol Toxicol · 2004 The family of adrenergic receptors (ARs) expressed in adipocytes includes three sibling betaARs and two alphaAR cousins. Together they profoundly influence the mobilization of stored fatty acids, secretion of fat-cell derived hormones, and the specialized ... Full text Link to item Cite

Regulation of the uncoupling protein-2 gene in INS-1 beta-cells by oleic acid.

Journal Article J Biol Chem · November 8, 2002 Current evidence suggests that uncoupling protein-2 (UCP2) is a regulator of insulin secretion. It is also known that chronic exposure of pancreatic islets to free fatty acids (FFAs) blunts glucose-stimulated insulin secretion and is accompanied by elevate ... Full text Link to item Cite

New series of aryloxypropanolamines with both human beta(3)-adrenoceptor agonistic activity and free radical scavenging properties.

Journal Article Bioorg Med Chem Lett · January 21, 2002 A series of 13 novel hybrid molecules designed to possess both free radical scavenging activity and to stimulate the beta(3)-adrenoceptors in order to improve antidiabetic effect and to restore insulin sensitivity was synthesized and evaluated. Compounds w ... Full text Link to item Cite

Adrenoceptors, uncoupling proteins, and energy expenditure.

Journal Article Exp Biol Med (Maywood) · December 2001 Interest in the biology of adipose tissue has undergone a revival in recent years with the discovery of a host of genes that contribute to the regulation of satiety and metabolic rate. The catecholamines have long been known to be key modulators of adipose ... Full text Link to item Cite

alpha(1)-adrenergic receptors mediate LH-releasing hormone secretion through phospholipases C and A(2) in immortalized hypothalamic neurons.

Journal Article Endocrinology · November 2001 Norepinephrine has long been known to stimulate the pulsatile and preovulatory release of LH-releasing hormone (LHRH). In vivo and in vitro studies indicate that these effects are mediated primarily through alpha(1)-adrenergic receptors (alpha(1)-ARs). Wit ... Full text Link to item Cite

beta-Adrenergic activation of p38 MAP kinase in adipocytes: cAMP induction of the uncoupling protein 1 (UCP1) gene requires p38 MAP kinase.

Journal Article J Biol Chem · July 20, 2001 Because of increasing evidence that G protein-coupled receptors activate multiple signaling pathways, it becomes important to determine the coordination of these pathways and their physiological significance. Here we show that the beta(3)-adrenergic recept ... Full text Link to item Cite

Revisiting lessons from the C57BL/6J mouse.

Journal Article Am J Physiol Endocrinol Metab · May 2001 Full text Link to item Cite

Transcriptional regulation of the mouse uncoupling protein-2 gene. Double E-box motif is required for peroxisome proliferator-activated receptor-gamma-dependent activation.

Journal Article J Biol Chem · April 6, 2001 Uncoupling protein-2 (UCP2) is present in many tissues with relevance to fuel metabolism, and its expression is increased in fat and muscle in response to elevated circulating free fatty acids resulting from fasting and high fat feeding. We proposed a role ... Full text Link to item Cite

CCAAT/enhancer-binding protein alpha is required for transcription of the beta 3-adrenergic receptor gene during adipogenesis.

Journal Article J Biol Chem · January 5, 2001 The beta(3)-adrenergic receptor (beta(3)AR) is expressed predominantly in adipocytes, and it plays a major role in regulating lipolysis and adaptive thermogenesis. Its expression in a variety of adipocyte cell models is preceded by the appearance of CCAAT/ ... Full text Link to item Cite

The beta-adrenergic receptors and the control of adipose tissue metabolism and thermogenesis.

Journal Article Recent Prog Horm Res · 2001 The beta-adrenergic receptors (betaARs) are members of the large family of G protein-coupled receptors. There are three betaAR subtypes (beta1AR, beta2AR beta3AR), each of which is coupled to Galphas and the stimulation of intracellular cAMP levels. While ... Full text Link to item Cite

Direct binding of activated c-Src to the beta 3-adrenergic receptor is required for MAP kinase activation.

Journal Article J Biol Chem · December 8, 2000 Both beta(2)- and beta(3)-adrenergic receptors (ARs) are able to activate the extracellular signal-regulated kinase (ERK) pathway. We previously showed that c-Src is required for ERK activation by beta(2)AR and that it is recruited to activated beta(2)AR t ... Full text Link to item Cite

Disruption of the uncoupling protein-2 gene in mice reveals a role in immunity and reactive oxygen species production.

Journal Article Nat Genet · December 2000 The gene Ucp2 is a member of a family of genes found in animals and plants, encoding a protein homologous to the brown fat uncoupling protein Ucp1 (refs 1-3). As Ucp2 is widely expressed in mammalian tissues, uncouples respiration and resides within a regi ... Full text Link to item Cite

Diazoxide restores beta3-adrenergic receptor function in diet-induced obesity and diabetes.

Journal Article Endocrinology · October 2000 We previously demonstrated that the expression and function of the adipocyte-specific beta3-adrenergic receptor (beta3AR) are significantly depressed in single gene and diet-induced rodent models of obesity. Furthermore, these models are relatively unrespo ... Full text Link to item Cite

Depressed expression of adipocyte beta-adrenergic receptors is a common feature of congenital and diet-induced obesity in rodents.

Journal Article Int J Obes Relat Metab Disord · July 1999 OBJECTIVE: To determine whether the dramatic reduction in expression and functional activity of the beta3-adrenergic receptor (AR) and beta1AR subtypes originally observed in adipose tissue of the C57BL/6J Lep(ob)/Lep(ob) ('obese') mouse are general featur ... Full text Link to item Cite

The beta3-adrenergic receptor activates mitogen-activated protein kinase in adipocytes through a Gi-dependent mechanism.

Journal Article J Biol Chem · April 23, 1999 Promiscuous coupling between G protein-coupled receptors and multiple species of heterotrimeric G proteins provides a potential mechanism for expanding the diversity of G protein-coupled receptor signaling. We have examined the mechanism and functional con ... Full text Link to item Cite

Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice.

Journal Article Proc Natl Acad Sci U S A · March 31, 1998 Uncoupling protein 2 (UCP2) maps to a region on distal mouse chromosome 7 that has been linked to the phenotypes of obesity and type II diabetes. We recently reported that UCP2 expression is increased by high fat feeding in adipose tissue of the A/J strain ... Full text Link to item Cite

Up-regulation of UCP-2 gene expression by PPAR agonists in preadipose and adipose cells.

Journal Article Biochem Biophys Res Commun · September 18, 1997 UCP-2 is a member of the emerging family of UCP homologues. Upon high-fat feeding, UCP-2 mRNA levels are increased in epididymal fat pads of A/J mice, suggesting that the flux of fatty acids entering adipose tissue may regulate UCP-2 gene expression. Since ... Full text Link to item Cite

Low plasma leptin in response to dietary fat in diabetes- and obesity-prone mice.

Journal Article Diabetes · September 1997 Despite the fact that mutations resulting in the absence of leptin or its receptor have been associated with severe obesity and diabetes, such mutations do not appear to be responsible for most human obesity. Indeed, diet-induced obesity in animals and hum ... Full text Link to item Cite

Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia.

Journal Article Nat Genet · March 1997 A mitochondrial protein called uncoupling protein (UCP1) plays an important role in generating heat and burning calories by creating a pathway that allows dissipation of the proton electrochemical gradient across the inner mitochondrial membrane in brown a ... Full text Link to item Cite

Strain-specific response to beta 3-adrenergic receptor agonist treatment of diet-induced obesity in mice.

Journal Article Endocrinology · January 1997 Fat intake has long been associated with the development of obesity. The studies described herein show that fat adversely affects adipocyte adrenergic receptor (AR) expression and function. As beta 3AR agonists have been shown to acutely reduce adipose tis ... Full text Link to item Cite

Role of leptin in fat regulation.

Journal Article Nature · April 25, 1996 Full text Link to item Cite

Pharmacologic manipulation of ob expression in a dietary model of obesity.

Journal Article J Biol Chem · April 19, 1996 Mutation of the obese (ob) gene results in severe hereditary obesity and diabetes in the C57BL/6J and related strains of mice. In this study we examined the expression of the ob gene in a dietary model in which moderate obesity develops in response to fat ... Full text Link to item Cite

Expression of candidate pro-GnRH processing enzymes in rat hypothalamus and an immortalized hypothalamic neuronal cell line.

Journal Article Neuroendocrinology · August 1995 Since gonadotropin-releasing hormone (GnRH, also referred to as LHRH) is a major hormone regulating mammalian reproduction, identification of the processing steps involved in the conversion of the pro-LHRH to LHRH is fundamental to our understanding of its ... Full text Link to item Cite

Regulation of the uncoupling protein gene (Ucp) by beta 1, beta 2, and beta 3-adrenergic receptor subtypes in immortalized brown adipose cell lines.

Journal Article J Biol Chem · May 5, 1995 Immortalized brown adipocyte cell lines derived from a mouse hibernoma express all three beta-adrenergic receptor subtypes, including beta 3-adrenergic receptor (AR). In response to norepinephrine, cAMP production by plasma membranes from four clonal cell ... Full text Link to item Cite

Impaired expression and functional activity of the beta 3- and beta 1-adrenergic receptors in adipose tissue of congenitally obese (C57BL/6J ob/ob) mice.

Journal Article Mol Endocrinol · April 1994 Adipocytes from genetically obese (ob/ob) mice display an impaired response to beta-adrenergic stimulation, but the molecular defects have not been unequivocally identified. The expression and functional activity of the beta 1-, beta 2-, and beta 3-adrener ... Full text Link to item Cite

Cloning and sequence analysis of the human beta 1-adrenergic receptor 5'-flanking promoter region.

Journal Article Biochim Biophys Acta · February 20, 1993 We present 3.1 kb of the nucleotide sequence from the 5'-flanking region of the human beta 1-adrenergic receptor gene. The first 1.0 kb upstream from the translational start site is composed of 72% G + C residues. The sequence was analyzed for the presence ... Full text Link to item Cite

Recent perspectives on the molecular structure and regulation of the beta 2-adrenoceptor.

Journal Article Life Sci · 1993 The recent cloning of the beta 2-AR and other members of the adrenergic receptor family and related molecules has generated a flood of new information about the structure of this ubiquitous class of receptor molecules, and the ways in which their unique st ... Full text Link to item Cite

Molecular structure of G-protein-coupled receptors and regulation of their expression

Journal Article Drug News and Perspectives · January 1, 1993 Cite

From ligand binding to gene expression: new insights into the regulation of G-protein-coupled receptors.

Journal Article Trends Biochem Sci · January 1992 Transmembrane signaling systems relay information from the exterior to the interior of a cell, through a series of complex protein-protein interactions and second messenger cascades. One such system consists of the G-protein-coupled receptors, which intera ... Full text Link to item Cite

Regulation of adrenergic receptor responsiveness through modulation of receptor gene expression.

Journal Article Annu Rev Physiol · 1991 Multiple mechanisms contribute to the regulation of G protein-coupled receptors and their transmembrane signaling. Post-translational modifications of the receptors, such as phosphorylation, and changes in receptor gene expression can occur in either a str ... Full text Link to item Cite

A cAMP response element in the beta 2-adrenergic receptor gene confers transcriptional autoregulation by cAMP.

Journal Article J Biol Chem · November 5, 1990 The rate of transcription of the beta 2-adrenergic receptor gene is increased in response to beta-adrenergic agonist stimulation of the receptor at the cell surface. This effect is mediated by stimulation of adenylyl cyclase and elevation of intracellular ... Link to item Cite

Expression of three alpha 2-adrenergic receptor subtypes in rat tissues: implications for alpha 2 receptor classification.

Journal Article Mol Pharmacol · November 1990 Based on biochemical and ligand binding studies in various tissues and species, evidence for several alpha 2-adrenergic receptor subtypes has accumulated. The current alpha 2-adrenergic receptor classification (alpha 2A, alpha 2B, alpha 2C) is based exclus ... Link to item Cite

Alpha 1-adrenergic receptor mRNA level is regulated by norepinephrine in rabbit aortic smooth muscle cells.

Journal Article Proc Natl Acad Sci U S A · August 1990 Prolonged agonist exposure results in a decrease in the density of alpha 1-adrenergic receptors in rabbit aortic smooth muscle cells. A cDNA for the alpha 1-adrenergic receptor was used to assess the effect of norepinephrine on alpha 1-adrenergic receptor ... Full text Link to item Cite

Two distinct pathways for cAMP-mediated down-regulation of the beta 2-adrenergic receptor. Phosphorylation of the receptor and regulation of its mRNA level.

Journal Article J Biol Chem · October 5, 1989 We have studied cyclic AMP-mediated regulation of the beta 2-adrenergic receptor (beta 2AR). The effects of cAMP were assessed in Chinese hamster fibroblast (CHW) cells expressing either the wild type human beta 2AR receptor (CH-beta 2) or mutated forms of ... Link to item Cite

cAMP stimulates transcription of the beta 2-adrenergic receptor gene in response to short-term agonist exposure.

Journal Article Proc Natl Acad Sci U S A · July 1989 In addition to conveying cellular responses to an effector molecule, receptors are often themselves regulated by their effectors. We have demonstrated that epinephrine modulates both the rate of transcription of the beta 2-adrenergic receptor (beta 2AR) ge ... Full text Open Access Link to item Cite

Genetic regulation of beta-adrenergic receptors.

Journal Article Annu Rev Physiol · 1989 Several members of the family of receptors coupled to G proteins have been cloned in recent years. From the primary sequence information furnished by these clones, a characteristic seven-membrane spanning topography has emerged as the prototype for this cl ... Full text Link to item Cite

Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.

Journal Article J Biol Chem · July 5, 1988 Steroid hormones modulate adrenergic receptor responsiveness and receptor number. To investigate the regulation of the beta 2-adrenergic receptor gene by glucocorticoids we examined the effects of the synthetic glucocorticoid agonist triamcinolone acetonid ... Link to item Cite

Regulation of the beta 2-adrenergic receptor and its mRNA in the rat ventral prostate by testosterone.

Journal Article FEBS Lett · June 6, 1988 To investigate the regulation of beta 2AR expression in rat ventral prostate the effects of castration and testosterone replacement on the beta 2AR were studied by ligand binding and Northern blot analysis. Orchidectomy depressed beta 2AR number by 50% wit ... Full text Link to item Cite

Expression of a human cDNA encoding the beta 2-adrenergic receptor in Chinese hamster fibroblasts (CHW): functionality and regulation of the expressed receptors.

Journal Article Mol Pharmacol · February 1988 A human beta-adrenergic receptor cDNA was transfected and expressed in transformed Chinese hamster fibroblasts (CHW). The expressed receptor exhibited a typical beta 2-adrenergic selectivity for agonists and antagonists as assessed by radioligand binding a ... Link to item Cite

β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids

Journal Article Journal of Biological Chemistry · January 1, 1988 Steroid hormones modulate adrenergic receptor responsiveness and receptor number. To investigate the regulation of the β2-adrenergic receptor gene by glucocorticoids we examined the effects of the synthetic glucocorticoid agonist triamcinolone acetonide on ... Cite

Cloning of the cDNA for the human beta 1-adrenergic receptor.

Journal Article Proc Natl Acad Sci U S A · November 1987 Screening of a human placenta lambda gt11 library has led to the isolation of the cDNA for the human beta 1-adrenergic receptor (beta 1AR). Used as the probe was the human genomic clone termed G-21. This clone, which contains an intronless gene for a putat ... Full text Open Access Link to item Cite

An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.

Journal Article Nature · September 3, 1987 Plasma membrane receptors for hormones, drugs, neurotransmitters and sensory stimuli are coupled to guanine nucleotide regulatory proteins. Recent cloning of the genes and/or cDNAs for several of these receptors including the visual pigment rhodopsin, the ... Full text Link to item Cite