Scholars@Duke
Shihuan Kuang

Shihuan Kuang

Professor of Orthopaedic Surgery
Orthopaedic Surgery
shihuan.kuang@duke.edu
308 Research Dr., LSRC B330C, Durham, NC 27710

Selected Publications

Lipid droplets as cell fate determinants in skeletal muscle.

Journal Article Trends Endocrinol Metab · November 28, 2024 Lipid droplets (LDs) are dynamic organelles that communicate with other cellular components to orchestrate energetic homeostasis and signal transduction. In skeletal muscle, the presence and importance of LDs have been widely studied in myofibers of both r ... Full text Link to item Cite

LETMD1 regulates mitochondrial protein synthesis and import to guard brown fat mitochondrial integrity and function.

Journal Article iScience · October 18, 2024 Thermogenic brown adipocytes (BAs) catabolize lipids to generate heat, representing powerful agents against the growing global obesity epidemic. We and others reported recently that LETMD1 is a BA-specific protein essential for mitochondrial structure and ... Full text Link to item Cite

Overexpression of CPT1A disrupts the maintenance and regenerative function of muscle stem cells.

Journal Article FASEB J · October 15, 2024 The skeletal muscle satellite cells (SCs) mediate regeneration of myofibers upon injury. As they switch from maintenance (quiescence) to regeneration, their relative reliance on glucose and fatty acid metabolism alters. To explore the contribution of mitoc ... Full text Link to item Cite

A Notch toward Progenitor D(G)FRentiation: Defining a NOTCH-PDGFR axis in brown adipogenesis.

Journal Article Dev Cell · May 20, 2024 Brown adipocytes are found in several fat depots, however, the origins and contributions of different lineages of adipogenic progenitor cells (APCs) to these depots are unclear. In this issue of Developmental Cell, Shi et al. show that platelet-derived gro ... Full text Link to item Cite

Monitoring drug metabolic pathways through extracellular vesicles in mouse plasma.

Journal Article PNAS Nexus · February 2024 The ability to monitor the response of metabolic enzymes to drug exposure in individuals is highly appealing and critical to personalized medicine. Although pharmacogenomics assesses genotypic differences, it does not report changes in metabolic enzyme act ... Full text Link to item Cite

Muscle stem cells as immunomodulator during regeneration.

Chapter · 2024 The skeletal muscle is well known for its remarkable ability to regenerate after injuries. The regeneration is a complex and dynamic process that involves muscle stem cells (also called muscle satellite cells, MuSCs), fibro-adipogenic progenitors (FAPs), i ... Full text Link to item Cite

PRMT5 mediates FoxO1 methylation and subcellular localization to regulate lipophagy in myogenic progenitors.

Journal Article Cell Rep · November 28, 2023 Development is regulated by various factors, including protein methylation status. While PRMT5 is well known for its roles in oncogenesis by mediating symmetric di-methylation of arginine, its role in normal development remains elusive. Using Myod1Cre to d ... Full text Link to item Cite

Angiogenic potential of skeletal muscle derived extracellular vesicles differs between oxidative and glycolytic muscle tissue in mice.

Journal Article Sci Rep · November 2, 2023 Skeletal muscle fibers regulate surrounding endothelial cells (EC) via secretion of numerous angiogenic factors, including extracellular vesicles (SkM-EV). Muscle fibers are broadly classified as oxidative (OXI) or glycolytic (GLY) depending on their metab ... Full text Link to item Cite

FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.

Journal Article Nat Commun · October 10, 2023 Cold stimulation dynamically remodels mitochondria in brown adipose tissue (BAT) to facilitate non-shivering thermogenesis in mammals, but what regulates mitochondrial plasticity is poorly understood. Comparing mitochondrial proteomes in response to cold r ... Full text Link to item Cite

A single-cell atlas of bovine skeletal muscle reveals mechanisms regulating intramuscular adipogenesis and fibrogenesis.

Journal Article J Cachexia Sarcopenia Muscle · October 2023 BACKGROUND: Intramuscular fat (IMF) and intramuscular connective tissue (IMC) are often seen in human myopathies and are central to beef quality. The mechanisms regulating their accumulation remain poorly understood. Here, we explored the possibility of us ... Full text Link to item Cite

Atypical peripheral actin band formation via overactivation of RhoA and nonmuscle myosin II in mitofusin 2-deficient cells.

Journal Article Elife · September 19, 2023 Cell spreading and migration play central roles in many physiological and pathophysiological processes. We have previously shown that MFN2 regulates the migration of human neutrophil-like cells via suppressing Rac activation. Here, we show that in mouse em ... Full text Link to item Cite

Sox11 is enriched in myogenic progenitors but dispensable for development and regeneration of the skeletal muscle.

Journal Article Skelet Muscle · September 13, 2023 Transcription factors (TFs) play key roles in regulating differentiation and function of stem cells, including muscle satellite cells (MuSCs), a resident stem cell population responsible for postnatal regeneration of the skeletal muscle. Sox11 belongs to t ... Full text Link to item Cite

PRMT5 links lipid metabolism to contractile function of skeletal muscles.

Journal Article EMBO Rep · August 3, 2023 Skeletal muscle plays a key role in systemic energy homeostasis besides its contractile function, but what links these functions is poorly defined. Protein Arginine Methyl Transferase 5 (PRMT5) is a well-known oncoprotein but also expressed in healthy tiss ... Full text Link to item Cite

Notch signaling regulates a metabolic switch through inhibiting PGC-1α and mitochondrial biogenesis in dedifferentiated liposarcoma.

Journal Article Oncogene · August 2023 Human dedifferentiated liposarcoma (DDLPS) is a rare but lethal cancer with no driver mutations being identified, hampering the development of targeted therapies. We and others recently reported that constitutive activation of Notch signaling through overe ... Full text Link to item Cite

Multimodal high-resolution nano-DESI MSI and immunofluorescence imaging reveal molecular signatures of skeletal muscle fiber types.

Journal Article Chem Sci · April 12, 2023 The skeletal muscle is a highly heterogeneous tissue comprised of different fiber types with varying contractile and metabolic properties. The complexity in the analysis of skeletal muscle fibers associated with their small size (30-50 μm) and mosaic-like ... Full text Link to item Cite

Peroxisome proliferator-activated receptor γ coactivator 1-α overexpression improves angiogenic signalling potential of skeletal muscle-derived extracellular vesicles.

Journal Article Exp Physiol · February 2023 NEW FINDINGS: What is the central question of this study? Skeletal muscle extracellular vesicles likely act as pro-angiogenic signalling factors: does overexpression of peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) alter skeletal mu ... Full text Link to item Cite

Hepatic Acat2 overexpression promotes systemic cholesterol metabolism and adipose lipid metabolism in mice.

Journal Article Diabetologia · February 2023 AIMS/HYPOTHESIS: Acetyl coenzyme A acetyltransferase (ACAT), also known as acetoacetyl-CoA thiolase, catalyses the formation of acetoacetyl-CoA from acetyl-CoA and forms part of the isoprenoid biosynthesis pathway. Thus, ACAT plays a central role in choles ... Full text Link to item Cite

Cellular and Transcriptional Dynamics during Brown Adipose Tissue Regeneration under Acute Injury.

Journal Article Research (Wash D C) · 2023 Brown adipose tissue (BAT) is the major site of non-shivering thermogenesis and crucial for systemic metabolism. Under chronic cold exposures and high-fat diet challenges, BAT undergoes robust remodeling to adapt to physiological demands. However, whether ... Full text Link to item Cite

Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.

Chapter · 2023 Lipid homeostasis is critical for maintaining normal cellular functions including membrane structural integrity, cell metabolism, and signal transduction. Adipose tissue and skeletal muscle are two major tissues involved in lipid metabolism. Adipose tissue ... Full text Link to item Cite

Labeling and analyzing lipid droplets in mouse muscle stem cells.

Journal Article STAR Protoc · December 16, 2022 Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol inclu ... Full text Link to item Cite

Proto-oncogene FAM83A contributes to casein kinase 1-mediated mitochondrial maintenance and white adipocyte differentiation.

Journal Article J Biol Chem · October 2022 Family with sequence similarity 83 A (FAM83A) is a newly discovered proto-oncogene that has been shown to play key roles in various cancers. However, the function of FAM83A in other physiological processes is not well known. Here, we report a novel functio ... Full text Link to item Cite

Effects of obesity and acute resistance exercise on skeletal muscle angiogenic communication pathways.

Journal Article Exp Physiol · August 2022 NEW FINDINGS: What is the central question of this study? Do obesity and acute resistance exercise alter the regulation of muscle intercellular communication pathways consistent with inadequate compensatory angiogenesis in response to muscle loading presen ... Full text Link to item Cite

Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells.

Journal Article Biomaterials · June 2022 Human hematopoietic stem cells (HSCs), which arise from aorta-gonad-mesonephros (AGM), are widely used to treat blood diseases and cancers. However, a technique for their robust generation in vitro is still missing. Here we show temporal manipulation of Wn ... Full text Link to item Cite

Obesity and exercise training alter inflammatory pathway skeletal muscle small extracellular vesicle microRNAs.

Journal Article Exp Physiol · May 2022 NEW FINDINGS: What is the central question of this study? Is 1 week of exercise training sufficient to reduce local and systemic inflammation? Do obesity and short-term concurrent aerobic and resistance exercise training alter skeletal muscle extracellular ... Full text Link to item Cite

Chchd10 is dispensable for myogenesis but critical for adipose browning.

Journal Article Cell Regen · April 1, 2022 The Chchd10 gene encodes a coiled-coil-helix-coiled-coil-helix-domain containing protein predicted to function in the mitochondrion and nucleus. Mutations of Chchd10 are associated with ALS, dementia and myopathy in humans and animal models, but how knocko ... Full text Link to item Cite

Lipid droplet dynamics regulate adult muscle stem cell fate.

Journal Article Cell Rep · January 18, 2022 The lipid droplet (LD) is a central hub for fatty acid metabolism in cells. Here we define the dynamics and explore the role of LDs in skeletal muscle satellite cells (SCs), a stem cell population responsible for muscle regeneration. In newly divided SCs, ... Full text Link to item Cite

LETMD1 is required for mitochondrial structure and thermogenic function of brown adipocytes.

Journal Article FASEB J · November 2021 Obesity and metabolic disorders caused by energy surplus pose an increasing concern within the global population. Brown adipose tissue (BAT) dissipates energy through mitochondrial non-shivering thermogenesis, thus representing a powerful agent against obe ... Full text Link to item Cite

Prenatal Serotonin Fluctuation Affects Serotoninergic Development and Related Neural Circuits in Chicken Embryos.

Journal Article Neuroscience · October 1, 2021 The placenta is the primary source of serotonin (5-HT) for fetal development, programming fetal neural wiring in humans and other mammals. The fluctuation in maternal 5-HT affects fetal neurogenesis with life-long consequences, however, its mechanisms have ... Full text Link to item Cite

Extracellular vesicles released from stress-induced prematurely senescent myoblasts impair endothelial function and proliferation.

Journal Article Exp Physiol · October 2021 NEW FINDINGS: What is the central question of this study? What is the impact of stress-induced premature senescence on skeletal muscle myoblast-derived extracellular vesicles (EVs) and myoblast-endothelial cell crosstalk? What is the main finding and its i ... Full text Link to item Cite

Reduced electron transport chain complex I protein abundance and function in Mfn2-deficient myogenic progenitors lead to oxidative stress and mitochondria swelling.

Journal Article FASEB J · April 2021 Mitochondrial remodeling through fusion and fission is crucial for progenitor cell differentiation but its role in myogenesis is poorly understood. Here, we characterized the function of mitofusin 2 (Mfn2), a mitochondrial outer membrane protein critical f ... Full text Link to item Cite

Biomimetic glycosaminoglycan-based scaffolds improve skeletal muscle regeneration in a Murine volumetric muscle loss model.

Journal Article Bioact Mater · April 2021 Volumetric muscle loss (VML) injuries characterized by critical loss of skeletal muscle tissues result in severe functional impairment. Current treatments involving use of muscle grafts are limited by tissue availability and donor site morbidity. In this s ... Full text Link to item Cite

Imaging and Analysis of Isomeric Unsaturated Lipids through Online Photochemical Derivatization of Carbon-Carbon Double Bonds*.

Journal Article Angew Chem Int Ed Engl · March 29, 2021 Unraveling the complexity of the lipidome requires the development of novel approaches for the structural characterization of lipid species with isomer-level discrimination. Herein, we introduce an online photochemical approach for lipid isomer identificat ... Full text Link to item Cite

Imaging and Analysis of Isomeric Unsaturated Lipids through Online Photochemical Derivatization of Carbon–Carbon Double Bonds**

Journal Article Angewandte Chemie · March 29, 2021 AbstractUnraveling the complexity of the lipidome requires the development of novel approaches for the structural characterization of lipid species with isomer‐level discrimination. Herein, we introduce an online photochemi ... Full text Cite

Harnessing nerve-muscle cell interactions for biomaterials-based skeletal muscle regeneration.

Journal Article J Biomed Mater Res A · March 2021 Nerve cells secrete neurotrophic factors that play a critical role in neuronal survival, proliferation, and regeneration. However, their role in regulating myoblast behavior and skeletal muscle repair remains largely unexplored. In the present study, we in ... Full text Link to item Cite

PTEN Inhibition Ameliorates Muscle Degeneration and Improves Muscle Function in a Mouse Model of Duchenne Muscular Dystrophy.

Journal Article Mol Ther · January 6, 2021 Duchenne muscular dystrophy (DMD) is caused by a mutation of the muscle membrane protein dystrophin and characterized by severe degeneration of myofibers, progressive muscle wasting, loss of mobility, and, ultimately, cardiorespiratory failure and prematur ... Full text Link to item Cite

Phosphatase orphan 1 inhibits myoblast proliferation and promotes myogenic differentiation.

Journal Article FASEB J · January 2021 Myogenesis includes sequential stages of progenitor cell proliferation, myogenic commitment and differentiation, myocyte fusion, and myotube maturation. Different stages of myogenesis are orchestrated and regulated by myogenic regulatory factors and variou ... Full text Link to item Cite

Polymeric nanoparticles functionalized with muscle-homing peptides for targeted delivery of phosphatase and tensin homolog inhibitor to skeletal muscle.

Journal Article Acta Biomater · December 2020 Phosphatase and tensin homolog (PTEN) antagonizes muscle growth and repair, and inhibition of PTEN has been shown to improve the pathophysiology and dystrophic muscle function in a mouse model of Duchenne muscular dystrophy (DMD). However, conventional pha ... Full text Link to item Cite

Protein Arginine Methyltransferase PRMT5 Regulates Fatty Acid Metabolism and Lipid Droplet Biogenesis in White Adipose Tissues.

Journal Article Adv Sci (Weinh) · December 2020 The protein arginine methyltransferase 5 (PRMT5) is an emerging regulator of cancer and stem cells including adipogenic progenitors. Here, a new physiological role of PRMT5 in adipocytes and systemic metabolism is reported. Conditional knockout mice were g ... Full text Link to item Cite

Skeletal muscle IGF-1 is lower at rest and after resistance exercise in humans with obesity.

Journal Article Eur J Appl Physiol · December 2020 PURPOSE: Obesity is associated with numerous changes in skeletal muscle including greater muscle mass and muscle fiber cross sectional area (FCSA), yet fasted muscle protein synthesis is lower. Activation of the IGF-1/Akt/mTOR pathway is critical for muscl ... Full text Link to item Cite

Sustained activation of notch signaling maintains tumor-initiating cells in a murine model of liposarcoma.

Journal Article Cancer Lett · December 1, 2020 Cells in a tumor are heterogeneous, often including a small number of tumor-initiating cells (TICs) and the majority of cancerous and non-cancerous cells. We have previously reported that constitutive activation of Notch signaling in adipocytes of mice lea ... Full text Link to item Cite

Exosomal Secretion of Adipose Tissue during Various Physiological States.

Journal Article Pharm Res · October 15, 2020 Exosomes are secreted extracellular vesicles containing a wide array of biologically active components. Recent studies have demonstrated that exosomes serve as an important vehicle for extracellular communication and exert systemic effects on the physiolog ... Full text Link to item Cite

Single-Cell Isolation from Regenerating Murine Muscles for RNA-Sequencing Analysis.

Journal Article STAR Protoc · September 18, 2020 Single-cell RNA sequencing (scRNA-seq) is a powerful technique for deconvoluting and clustering thousands of otherwise intermingled cells based on their gene expression. Here, we present a complete protocol for the unbiased evaluation of regenerating murin ... Full text Link to item Cite

In Vitro Evaluation of Clinical Candidates of γ-Secretase Inhibitors: Effects on Notch Inhibition and Promoting Beige Adipogenesis and Mitochondrial Biogenesis.

Journal Article Pharm Res · September 4, 2020 PURPOSE: Inhibition of Notch signaling has been recently demonstrated to promote beige adipocyte biogenesis. However, most γ-secretase inhibitors (GSIs) used to achieve pharmacological inhibition of Notch signaling are at the basic research or preclinical ... Full text Link to item Cite

One-to-one relationships between milk miRNA content and protein abundance in neonate duodenum support the potential for milk miRNAs regulating neonate development.

Journal Article Funct Integr Genomics · September 2020 Breast milk plays an essential role for offspring development; however, there lacks evidence of how specific milk components like nucleic acids mechanistically function to regulate neonate development. Previously, we found that maternal high-fat diet (HFD) ... Full text Link to item Cite

Isolation, Culture, and Differentiation of Primary Myoblasts Derived from Muscle Satellite Cells.

Journal Article Bio Protoc · July 20, 2020 The skeletal muscle is key for body mobility and motor performance, but aging and diseases often lead to progressive loss of muscle mass due to wasting or degeneration of muscle cells. Muscle satellite cells (MuSCs) represent a population of tissue stem ce ... Full text Link to item Cite

Nanoparticle-Mediated Inhibition of Notch Signaling Promotes Mitochondrial Biogenesis and Reduces Subcutaneous Adipose Tissue Expansion in Pigs.

Journal Article iScience · June 26, 2020 Inhibition of Notch signaling has been shown to induce white to beige transformation of adipocytes and reduce the risk of obesity in mice. However, it remains unknown whether the metabolic benefits of Notch inhibition are dependent on uncoupling protein 1 ... Full text Link to item Cite

Temporal Dynamics and Heterogeneity of Cell Populations during Skeletal Muscle Regeneration.

Journal Article iScience · April 24, 2020 Mammalian skeletal muscle possesses a unique ability to regenerate, which is primarily mediated by a population of resident muscle stem cells (MuSCs) and requires a concerted response from other supporting cell populations. Previous targeted analysis has d ... Full text Link to item Cite

Effects of repeated local heat therapy on skeletal muscle structure and function in humans.

Journal Article J Appl Physiol (1985) · March 1, 2020 The purpose of the present study was to examine the effects of repeated exposure to local heat therapy (HT) on skeletal muscle function, myofiber morphology, capillarization, and mitochondrial content in humans. Twelve young adults (23.6 ± 4.8 yr, body mas ... Full text Link to item Cite

Multivesicular body and exosome pathway responses to acute exercise.

Journal Article Exp Physiol · March 2020 NEW FINDINGS: What is the central question of this study? What is the impact of acute aerobic and aerobic + resistance (concurrent) exercise on the regulation of multivesicular body formation in human skeletal muscle? What is the main finding and its impor ... Full text Link to item Cite

Imaging of Triglycerides in Tissues Using Nanospray Desorption Electrospray Ionization (Nano-DESI) Mass Spectrometry.

Journal Article Int J Mass Spectrom · February 2020 Nonpolar triglycerides (TGs) are rarely detected in mass spectrometry imaging (MSI) experiments unless they are abundant in the sample. Herein, we use nanospray desorption electrospray ionization (nano-DESI) to explore the role of the solvent composition a ... Full text Link to item Cite

Harnessing Fiber Diameter-Dependent Effects of Myoblasts Toward Biomimetic Scaffold-Based Skeletal Muscle Regeneration.

Journal Article Front Bioeng Biotechnol · 2020 Regeneration of skeletal muscles is limited in cases of volumetric muscle loss and muscle degenerative diseases. Therefore, there is a critical need for developing strategies that provide cellular and structural support for skeletal muscle regeneration. In ... Full text Link to item Cite

Polymeric Carriers for Controlled Drug Delivery in Obesity Treatment.

Journal Article Trends Endocrinol Metab · December 2019 The global rise in the prevalence of obesity and affiliated metabolic syndrome poses a significant threat to human health. Various approaches, including bariatric surgery and pharmacotherapy, have been used in the clinical setting for obesity treatment; ho ... Full text Link to item Cite

Adipocyte dedifferentiation in health and diseases.

Journal Article Clin Sci (Lond) · October 30, 2019 Adipose tissues collectively as an endocrine organ and energy storage are crucial for systemic metabolic homeostasis. The major cell type in the adipose tissue, the adipocytes or fat cells, are remarkably plastic and can increase or decrease their size and ... Full text Link to item Cite

Factors secreted from high glucose treated endothelial cells impair expansion and differentiation of human skeletal muscle satellite cells.

Journal Article J Physiol · October 2019 KEY POINTS: Cellular communication occurs between endothelial cells and skeletal muscle satellite cells and is mitogenic for both cell types under normal conditions. Skeletal muscle atrophy and endothelial cell dysfunction occur in tandem in cardiovascular ... Full text Link to item Cite

Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice.

Journal Article J Biol Chem · September 13, 2019 Protein methyltransferases mediate posttranslational modifications of both histone and nonhistone proteins. Whereas histone methylation is well-known to regulate gene expression, the biological significance of nonhistone methylation is poorly understood. M ... Full text Link to item Cite

Advanced Glycation End-Products Suppress Mitochondrial Function and Proliferative Capacity of Achilles Tendon-Derived Fibroblasts.

Journal Article Sci Rep · August 30, 2019 Debilitating cases of tendon pain and degeneration affect the majority of diabetic individuals. The high rate of tendon degeneration persists even when glucose levels are well controlled, suggesting that other mechanisms may drive tendon degeneration in di ... Full text Link to item Cite

A requirement of Polo-like kinase 1 in murine embryonic myogenesis and adult muscle regeneration.

Journal Article Elife · August 8, 2019 Muscle development and regeneration require delicate cell cycle regulation of embryonic myoblasts and adult muscle satellite cells (MuSCs). Through analysis of the Polo-like kinase (Plk) family cell-cycle regulators in mice, we show that Plk1's expression ... Full text Link to item Cite

Methyltransferase-like 21e inhibits 26S proteasome activity to facilitate hypertrophy of type IIb myofibers.

Journal Article FASEB J · August 2019 Skeletal muscles contain heterogeneous myofibers that are different in size and contractile speed, with type IIb myofiber being the largest and fastest. Here, we identify methyltransferase-like 21e (Mettl21e), a member of newly classified nonhistone methyl ... Full text Link to item Cite

Effects of acute aerobic and concurrent exercise on skeletal muscle metabolic enzymes in untrained men

Journal Article Sport Sciences for Health · August 1, 2019 Background: Acute exercise can increase skeletal muscle citrate synthase (CS) enzyme activity and resting skeletal muscle mitochondrial enzyme activity has been linked to maximal oxygen consumption (V˙ O 2 max). We investigated: (1) if acute aerobic exerci ... Full text Cite

Skeletal muscle-derived exosomes regulate endothelial cell functions via reactive oxygen species-activated nuclear factor-κB signalling.

Journal Article Exp Physiol · August 2019 NEW FINDINGS: What is the central question of this study? Capillary rarefaction is found in diabetic and aged muscle, whereas exercise increases skeletal muscle angiogenesis. The association implies a crosstalk between muscle cells and endothelial cells. T ... Full text Link to item Cite

The development of the serotonergic and dopaminergic systems during chicken mid-late embryogenesis.

Journal Article Mol Cell Endocrinol · August 1, 2019 Serotonin (5-HT) acts as a morphogen influencing embryonic brain development, and as a neurotransmitter regulating multiple biological functions with lifelong effects on animal physical, physiological and mental health, especially during the rapid growth p ... Full text Link to item Cite

Heat therapy improves soleus muscle force in a model of ischemia-induced muscle damage.

Journal Article J Appl Physiol (1985) · July 1, 2019 Leg muscle ischemia in patients with peripheral artery disease (PAD) leads to alterations in skeletal muscle morphology and reduced leg strength. We tested the hypothesis that exposure to heat therapy (HT) would improve skeletal muscle function in a mouse ... Full text Link to item Cite

Nanosecond pulsed electric field induced proliferation and differentiation of osteoblasts and myoblasts.

Journal Article J R Soc Interface · June 28, 2019 Low-intensity electric fields can induce changes in cell differentiation and cytoskeletal stresses that facilitate manipulation of osteoblasts and mesenchymal stem cells; however, the application times (tens of minutes) are of the order of physiological me ... Full text Link to item Cite

Maternal high-fat diet exposure during gestation, lactation, or gestation and lactation differentially affects intestinal morphology and proteome of neonatal mice.

Journal Article Nutr Res · June 2019 Offspring nutrition depends on the mother during gestation and lactation; thus, maternal nutrition and metabolism can affect their development. We hypothesized that maternal exposure to high-fat (HF) diet affects neonate's gastrointestinal tract developmen ... Full text Link to item Cite

Fndc5 loss-of-function attenuates exercise-induced browning of white adipose tissue in mice.

Journal Article FASEB J · May 2019 Fibronectin type III domain containing 5 (Fndc5) is a transmembrane protein highly expressed in the skeletal muscle. It was reported that exercise promotes the shedding of the extracellular domain of Fndc5, generating a circulating peptide (irisin) that cr ... Full text Link to item Cite

Impact of heat therapy on recovery after eccentric exercise in humans.

Journal Article J Appl Physiol (1985) · April 1, 2019 The purpose of this study was to investigate the effects of heat therapy (HT) on functional recovery, the skeletal muscle expression of angiogenic factors, macrophage content, and capillarization after eccentric exercise in humans. Eleven untrained individ ... Full text Link to item Cite

Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD.

Chapter · 2019 Brown adipocytes dissipate energy through non-shivering thermogenesis mediated by UCP1 protein, hence representing a powerful target to overcome obesity due to energy surplus. However, brown adipocytes are scarce in adult humans, especially in obese subjec ... Full text Link to item Cite

Early detection and monitoring of chronic wounds using low-cost, omniphobic paper-based smart bandages.

Journal Article Biosens Bioelectron · October 15, 2018 The growing socio-economic burden of chronic skin wounds requires the development of new automated and non-invasive analytical systems capable of wirelessly monitoring wound status. This work describes the low-cost fabrication of single-use, omniphobic pap ... Full text Link to item Cite

Wearable and Implantable Epidermal Paper-Based Electronics.

Journal Article ACS Appl Mater Interfaces · September 19, 2018 Traditional manufacturing methods and materials used to fabricate epidermal electronics for physiological monitoring, transdermal stimulation, and therapeutics are complex and expensive, preventing their adoption as single-use medical devices. This work de ... Full text Link to item Cite

Inhibition of cholesterol biosynthesis overcomes enzalutamide resistance in castration-resistant prostate cancer (CRPC).

Journal Article J Biol Chem · September 14, 2018 Enzalutamide, a nonsteroidal second-generation antiandrogen, has been recently approved for the management of castration-resistant prostate cancer (CRPC). Although patients can benefit from enzalutamide at the beginning of this therapy, acquired enzalutami ... Full text Link to item Cite

Shisa2 regulates the fusion of muscle progenitors.

Journal Article Stem Cell Res · August 2018 Adult skeletal muscles are comprised of multinuclear muscle cells called myofibers. During skeletal muscle development and regeneration, mononuclear progenitor cells (myoblasts) fuse to form multinuclear myotubes, which mature and become myofibers. The mol ... Full text Link to item Cite

Adipocyte-specific DKO of Lkb1 and mTOR protects mice against HFD-induced obesity, but results in insulin resistance.

Journal Article J Lipid Res · June 2018 Liver kinase B1 (Lkb1) and mammalian target of rapamycin (mTOR) are key regulators of energy metabolism and cell growth. We have previously reported that adipocyte-specific KO of Lkb1 or mTOR in mice results in distinct developmental and metabolic phenotyp ... Full text Link to item Cite

Enhanced Mechanical and Biological Performance of an Extremely Fine Nanograined 316L Stainless Steel Cell-Substrate Interface Fabricated by Ultrasonic Shot Peening.

Journal Article ACS Biomater Sci Eng · May 14, 2018 An extremely fine nanograined (NG) rough surface with the average grain size of 10 nm was successfully fabricated on 316L stainless steel (316L SS), which is a commonly used bioimplant metallic materials, via a simple physical therapy, namely, ultrasonic s ... Full text Link to item Cite

Identification of genes directly responding to DLK1 signaling in Callipyge sheep.

Journal Article BMC Genomics · April 24, 2018 BACKGROUND: In food animal agriculture, there is a need to identify the mechanisms that can improve the efficiency of muscle growth and protein accretion. Callipyge sheep provide excellent machinery since the up-regulation of DLK1 and RTL1 results in extre ... Full text Link to item Cite

A novel brown adipocyte-enriched long non-coding RNA that is required for brown adipocyte differentiation and sufficient to drive thermogenic gene program in white adipocytes.

Journal Article Biochim Biophys Acta Mol Cell Biol Lipids · April 2018 The thermogenic activities of brown and beige adipocytes can be exploited to reduce energy surplus and counteract obesity. Recent RNA sequencing studies have uncovered a number of long noncoding RNAs (lncRNAs) uniquely expressed in white and brown adipose ... Full text Link to item Cite

High Incomplete Skeletal Muscle Fatty Acid Oxidation Explains Low Muscle Insulin Sensitivity in Poorly Controlled T2D.

Journal Article J Clin Endocrinol Metab · March 1, 2018 CONTEXT: Almost 50% of type 2 diabetic (T2D) patients are poorly controlled [glycated hemoglobin (HbA1c) ≥ 7%]; however, the mechanisms responsible for progressively worsening glycemic control are poorly understood. Lower skeletal muscle mitochondrial resp ... Full text Link to item Cite

Notoginsenoside R1 Protects Against Diabetic Cardiomyopathy Through Activating Estrogen Receptor α and Its Downstream Signaling.

Journal Article Front Pharmacol · 2018 Diabetic cardiomyopathy (DCM) leads to heart failure and death in diabetic patients, no effective treatment is available. Notoginsenoside R1 (NGR1) is a novel saponin that is derived from Panax notoginseng and our previous studies have showed cardioprotect ... Full text Link to item Cite

Effect of high-fat diet on secreted milk transcriptome in midlactation mice.

Journal Article Physiol Genomics · December 1, 2017 High-fat diet (HFD) during lactation alters milk composition and is associated with development of metabolic diseases in the offspring. We hypothesized that HFD affects milk microRNA (miRNA) and mRNA content, which potentially impact offspring development. ... Full text Link to item Cite

Peripheral Neuropathy and Hindlimb Paralysis in a Mouse Model of Adipocyte-Specific Knockout of Lkb1.

Journal Article EBioMedicine · October 2017 Brown adipose tissues (BAT) burn lipids to generate heat through uncoupled respiration, thus representing a powerful target to counteract lipid accumulation and obesity. The tumor suppressor liver kinase b1 (Lkb1) is a key regulator of cellular energy meta ... Full text Link to item Cite

Depot-specific differences in fat mass expansion in WT and ob/ob mice.

Journal Article Oncotarget · July 11, 2017 The study was designed to investigate the cellular mechanisms underlying the differential fat expansion in different fat depots in wild type (WT) and ob/ob (OB) mice. At 6 weeks old, no differences in fat mass were found between SAT and VAT in WT mice, whi ... Full text Link to item Cite

Dibenzazepine-Loaded Nanoparticles Induce Local Browning of White Adipose Tissue to Counteract Obesity.

Journal Article Mol Ther · July 5, 2017 Inhibition of Notch signaling via systemic drug administration triggers conversion of white adipocytes into beige adipocytes (browning) and reduces adiposity. However, translation of this discovery into clinical practice is challenged by potential off-targ ... Full text Link to item Cite

The hypoxia-inducible factors HIF1α and HIF2α are dispensable for embryonic muscle development but essential for postnatal muscle regeneration.

Journal Article J Biol Chem · April 7, 2017 Muscle satellite cells are myogenic stem cells whose quiescence, activation, self-renewal, and differentiation are influenced by oxygen supply, an environmental regulator of stem cell activity. Accordingly, stem cell-specific oxygen signaling pathways prec ... Full text Link to item Cite

Myricitrin Alleviates Oxidative Stress-induced Inflammation and Apoptosis and Protects Mice against Diabetic Cardiomyopathy.

Journal Article Sci Rep · March 13, 2017 Diabetic cardiomyopathy (DCM) has been increasingly considered as a main cause of heart failure and death in diabetic patients. At present, no effective treatment exists to prevent its development. In the present study, we describe the potential protective ... Full text Link to item Cite

Enhanced human osteoblast cell functions by “net-like” nanostructured cell-substrate interface in orthopedic applications

Journal Article Materials Letters · February 15, 2017 In this study, a novel “net-like” nanostructured cell-substrate interface to enhance the human osteoblast cell's functions in orthopedic applications was proposed. This novel “net-like” nanostructured surface was fabricated on 316L stainless steel by using ... Full text Cite

Loss of MyoD Promotes Fate Transdifferentiation of Myoblasts Into Brown Adipocytes.

Journal Article EBioMedicine · February 2017 Brown adipose tissue (BAT) represents a promising agent to ameliorate obesity and other metabolic disorders. However, the abundance of BAT decreases with age and BAT paucity is a common feature of obese subjects. As brown adipocytes and myoblasts share a c ... Full text Link to item Cite

Stimulated Raman scattering flow cytometry for label-free single-particle analysis.

Journal Article Optica · January 20, 2017 Flow cytometry is one of the most important technologies for high-throughput single-cell analysis. Fluorescent labeling acts as the primary approach for cellular analysis in flow cytometry. Nevertheless, the fluorescent tags are not applicable to all cases ... Full text Link to item Cite

Pten is necessary for the quiescence and maintenance of adult muscle stem cells.

Journal Article Nat Commun · January 17, 2017 Satellite cells (SCs) are myogenic stem cells required for regeneration of adult skeletal muscles. A proper balance among quiescence, activation and differentiation is essential for long-term maintenance of SCs and their regenerative function. Here we show ... Full text Link to item Cite

Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors.

Journal Article Development · January 15, 2017 Myogenic regulatory factors (MRFs), including Myf5, MyoD (Myod1) and Myog, are muscle-specific transcription factors that orchestrate myogenesis. Although MRFs are essential for myogenic commitment and differentiation, timely repression of their activity i ... Full text Link to item Cite

Conditional Loss of Pten in Myogenic Progenitors Leads to Postnatal Skeletal Muscle Hypertrophy but Age-Dependent Exhaustion of Satellite Cells.

Journal Article Cell Rep · November 22, 2016 Skeletal muscle stem cells (satellite cells [SCs]) are normally maintained in a quiescent (G0) state. Muscle injury not only activates SCs locally, but also alerts SCs in distant uninjured muscles via circulating factors. The resulting GAlert SCs are adapt ... Full text Link to item Cite

Deletion of Lkb1 in adult mice results in body weight reduction and lethality.

Journal Article Sci Rep · November 8, 2016 Liver kinase B1 (Lkb1) plays crucial roles in development, metabolism and survival. As constitutive knockout of Lkb1 in mice leads to embryonic lethality, whether Lkb1 is required for the growth and survival of adult mice is unclear. Here we address this q ... Full text Link to item Cite

Impaired exercise tolerance, mitochondrial biogenesis, and muscle fiber maintenance in miR-133a-deficient mice.

Journal Article FASEB J · November 2016 Exercise promotes multiple beneficial effects on muscle function, including induction of mitochondrial biogenesis. miR-133a is a muscle-enriched microRNA that regulates muscle development and function. The role of miR-133a in exercise tolerance has not bee ... Full text Link to item Cite

Stage-specific effects of Notch activation during skeletal myogenesis.

Journal Article Elife · September 19, 2016 Skeletal myogenesis involves sequential activation, proliferation, self-renewal/differentiation and fusion of myogenic stem cells (satellite cells). Notch signaling is known to be essential for the maintenance of satellite cells, but its function in late-s ... Full text Link to item Cite

Notch activation drives adipocyte dedifferentiation and tumorigenic transformation in mice.

Journal Article J Exp Med · September 19, 2016 Liposarcomas (LPSs) are the most common soft-tissue cancer. Because of the lack of animal models, the cellular origin and molecular regulation of LPS remain unclear. Here, we report that mice with adipocyte-specific activation of Notch signaling (Ad/N1ICD) ... Full text Link to item Cite

Adipocyte-specific deletion of mTOR inhibits adipose tissue development and causes insulin resistance in mice.

Journal Article Diabetologia · September 2016 AIMS/HYPOTHESIS: The in vivo role of mechanistic target of rapamycin (mTOR) in the development and function of adipose tissue, especially brown adipose tissue (BAT), is not well understood. Here, we aimed to assess the effect of mTOR (also known as Mtor) k ... Full text Link to item Cite

Heat therapy promotes the expression of angiogenic regulators in human skeletal muscle.

Journal Article Am J Physiol Regul Integr Comp Physiol · August 1, 2016 Heat therapy has been shown to promote capillary growth in skeletal muscle and in the heart in several animal models, but the effects of this therapy on angiogenic signaling in humans are unknown. We evaluated the acute effect of lower body heating (LBH) a ... Full text Link to item Cite

Lkb1 controls brown adipose tissue growth and thermogenesis by regulating the intracellular localization of CRTC3.

Journal Article Nat Commun · July 27, 2016 Brown adipose tissue (BAT) dissipates energy through Ucp1-mediated uncoupled respiration and its activation may represent a therapeutic strategy to combat obesity. Here we show that Lkb1 controls BAT expansion and UCP1 expression in mice. We generate adipo ... Full text Link to item Cite

Stilbenoids remodel the DNA methylation patterns in breast cancer cells and inhibit oncogenic NOTCH signaling through epigenetic regulation of MAML2 transcriptional activity.

Journal Article Carcinogenesis · July 2016 DNA hypomethylation was previously implicated in cancer progression and metastasis. The purpose of this study was to examine whether stilbenoids, resveratrol and pterostilbene thought to exert anticancer effects, target genes with oncogenic function for de ... Full text Link to item Cite

Lkb1 deletion upregulates Pax7 expression through activating Notch signaling pathway in myoblasts.

Journal Article Int J Biochem Cell Biol · July 2016 Satellite cells play crucial roles in mediating the growth, maintenance, and repair of postnatal skeletal muscle. Activated satellite cells (myoblasts) can divide symmetrically or asymmetrically to generate progenies that self-renewal, proliferate or diffe ... Full text Link to item Cite

The brain expressed x-linked gene 1 (Bex1) regulates myoblast fusion.

Journal Article Dev Biol · January 1, 2016 Skeletal muscle development (myogenesis) is a complex but precisely orchestrated process involving spatiotemporal regulation of the proliferation, differentiation and fusion of myogenic progenitor cells (myoblasts). Here we identify brain expressed x-linke ... Full text Link to item Cite

Hypoxia Inhibits Myogenic Differentiation through p53 Protein-dependent Induction of Bhlhe40 Protein.

Journal Article J Biol Chem · December 11, 2015 Satellite cells are muscle-resident stem cells capable of self-renewal and differentiation to repair injured muscles. However, muscle injury often leads to an ischemic hypoxia environment that impedes satellite cell differentiation and reduces the efficien ... Full text Link to item Cite

AMP-Activated Protein Kinase Directly Phosphorylates and Destabilizes Hedgehog Pathway Transcription Factor GLI1 in Medulloblastoma.

Journal Article Cell Rep · July 28, 2015 The Hedgehog (Hh) pathway regulates cell differentiation and proliferation during development by controlling the Gli transcription factors. Cell fate decisions and progression toward organ and tissue maturity must be coordinated, and how an energy sensor r ... Full text Link to item Cite

mTOR is necessary for proper satellite cell activity and skeletal muscle regeneration.

Journal Article Biochem Biophys Res Commun · July 17, 2015 The serine/threonine kinase mammalian target of rapamycin (mTOR) is a key regulator of protein synthesis, cell proliferation and energy metabolism. As constitutive deletion of Mtor gene results in embryonic lethality, the function of mTOR in muscle stem ce ... Full text Link to item Cite

Heterogeneous activation of a slow myosin gene in proliferating myoblasts and differentiated single myofibers.

Journal Article Dev Biol · June 1, 2015 Each skeletal muscle contains a fixed ratio of fast and slow myofibers that are distributed in a stereotyped pattern to achieve a specific motor function. How myofibers are specified during development and regeneration is poorly understood. Here we address ... Full text Link to item Cite

Notch signaling as a novel regulator of metabolism.

Journal Article Trends Endocrinol Metab · May 2015 Evolutionarily unprepared for modern high-calorie diets and sedentary lifestyles, humans are now unprecedentedly susceptible to metabolic disorders such as obesity, type 2 diabetes (T2D), nonalcoholic fatty liver, and cardiovascular disease. These metaboli ... Full text Link to item Cite

Lkb1 deletion promotes ectopic lipid accumulation in muscle progenitor cells and mature muscles.

Journal Article J Cell Physiol · May 2015 Excessive intramyocellular triglycerides (muscle lipids) are associated with reduced contractile function, insulin resistance, and Type 2 diabetes, but what governs lipid accumulation in muscle is unclear. Here we report a role of Lkb1 in regulating lipid ... Full text Link to item Cite

Inhibition of polo-like kinase 1 (Plk1) enhances the antineoplastic activity of metformin in prostate cancer.

Journal Article J Biol Chem · January 23, 2015 The widely used anti-diabetic drug metformin has been shown to exert strong antineoplastic actions in numerous tumor types, including prostate cancer (PCa). In this study, we show that BI2536, a specific Plk1 inhibitor, acted synergistically with metformin ... Full text Link to item Cite

Biodegradable Polymeric Microsphere-Based Drug Delivery for Inductive Browning of Fat.

Journal Article Front Endocrinol (Lausanne) · 2015 Brown and beige adipocytes are potent therapeutic agents to increase energy expenditure and reduce risks of obesity and its affiliated metabolic symptoms. One strategy to increase beige adipocyte content is through inhibition of the evolutionarily conserve ... Full text Link to item Cite

Plk1 inhibition enhances the efficacy of androgen signaling blockade in castration-resistant prostate cancer.

Journal Article Cancer Res · November 15, 2014 Prostate cancer is thought to be driven by oxidative stress, lipid metabolism, androgen receptor (AR) signaling, and activation of the PI3K-AKT-mTOR pathway, but it is uncertain how they may become coordinated during progression to castration-resistant dis ... Full text Link to item Cite

Lkb1 is indispensable for skeletal muscle development, regeneration, and satellite cell homeostasis.

Journal Article Stem Cells · November 2014 Serine/threonine kinase 11, commonly known as liver kinase b1 (Lkb1), is a tumor suppressor that regulates cellular energy metabolism and stem cell function. Satellite cells are skeletal muscle resident stem cells that maintain postnatal muscle growth and ... Full text Link to item Cite

Plk1 phosphorylation of PTEN causes a tumor-promoting metabolic state.

Journal Article Mol Cell Biol · October 1, 2014 One outcome of activation of the phosphatidylinositol 3-kinase (PI3K) pathway is increased aerobic glycolysis, but the upstream signaling events that regulate the PI3K pathway, and thus the Warburg effect, are elusive. Increasing evidence suggests that Plk ... Full text Link to item Cite

Mammalian target of rapamycin is essential for cardiomyocyte survival and heart development in mice.

Journal Article Biochem Biophys Res Commun · September 12, 2014 Mammalian target of rapamycin (mTOR) is a critical regulator of protein synthesis, cell proliferation and energy metabolism. As constitutive knockout of Mtor leads to embryonic lethality, the in vivo function of mTOR in perinatal development and postnatal ... Full text Link to item Cite

Notch signaling deficiency underlies age-dependent depletion of satellite cells in muscular dystrophy.

Journal Article Dis Model Mech · August 2014 Duchenne muscular dystrophy (DMD) is a devastating disease characterized by muscle wasting, loss of mobility and death in early adulthood. Satellite cells are muscle-resident stem cells responsible for the repair and regeneration of damaged muscles. One pa ... Full text Link to item Cite

Inhibition of Notch signaling promotes browning of white adipose tissue and ameliorates obesity.

Journal Article Nat Med · August 2014 Beige adipocytes in white adipose tissue (WAT) are similar to classical brown adipocytes in that they can burn lipids to produce heat. Thus, an increase in beige adipocyte content in WAT browning would raise energy expenditure and reduce adiposity. Here we ... Full text Link to item Cite

Fighting obesity: When muscle meets fat.

Journal Article Adipocyte · 2014 The prevalence of obesity has risen to an unprecedented level. According to World Health Organization, over 500 million adults, equivalent to 10%-14% of the world population, were obese with a body mass index (BMI) of 30 kg/m(2) or greater in 2008.(1) This ... Full text Link to item Cite

Park7 expression influences myotube size and myosin expression in muscle.

Journal Article PLoS One · 2014 Callipyge sheep exhibit postnatal muscle hypertrophy due to the up-regulation of DLK1 and/or RTL1. The up-regulation of PARK7 was identified in hypertrophied muscles by microarray analysis and further validated by quantitative PCR. The expression of PARK7 ... Full text Link to item Cite

Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-κB signaling pathways.

Journal Article J Biol Chem · December 6, 2013 Satellite cell self-renewal is an essential process to maintaining the robustness of skeletal muscle regenerative capacity. However, extrinsic factors that regulate self-renewal of satellite cells are not well understood. Here, we demonstrate that TWEAK cy ... Full text Link to item Cite

Porcine satellite cells are restricted to a phenotype resembling their muscle origin.

Journal Article J Anim Sci · October 2013 Muscles in most domestic animals differ in function and growth potential based largely on muscle fiber type composition. Though much is known about satellite cells (SC), information is limited regarding how populations of SC differ with muscle fiber type, ... Full text Link to item Cite

Plk1-dependent microtubule dynamics promotes androgen receptor signaling in prostate cancer.

Journal Article Prostate · September 2013 BACKGROUND: The androgen receptor (AR) signaling continues to be essential in castrate-resistant prostate cancer (CRPC). Taxel-based chemotherapy is the current standard treatment for CRPC patients. Unfortunately, almost all patients eventually develop res ... Full text Link to item Cite

A heterogeneous lineage origin underlies the phenotypic and molecular differences of white and beige adipocytes.

Journal Article J Cell Sci · August 15, 2013 A worldwide epidemic of obesity and its associated metabolic disorders raise the significance of adipocytes, their origins and characteristics. Our previous study has demonstrated that interscapular brown adipose tissue (BAT), but not intramuscular adipose ... Full text Link to item Cite

Distinct populations of adipogenic and myogenic Myf5-lineage progenitors in white adipose tissues.

Journal Article J Lipid Res · August 2013 Brown adipose tissues (BAT) are derived from a myogenic factor 5 (Myf5)-expressing cell lineage and white adipose tissues (WAT) predominantly arise from non-Myf5 lineages, although a subpopulation of adipocytes in some WAT depots can be derived from the My ... Full text Link to item Cite

Myostatin knockout drives browning of white adipose tissue through activating the AMPK-PGC1α-Fndc5 pathway in muscle.

Journal Article FASEB J · May 2013 Myostatin (Mstn) is predominantly expressed in skeletal muscles and plays important roles in regulating muscle growth and development, as well as fat deposition. Mstn-knockout (Mstn(-/-)) mice exhibit increased muscle mass due to both hypertrophy and hyper ... Full text Link to item Cite

Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts.

Journal Article Skelet Muscle · March 5, 2013 BACKGROUND: The Wnts are secreted proteins that play important roles in skeletal myogenesis, muscle fiber type diversification, neuromuscular junction formation and muscle stem cell function. How Wnt proteins orchestrate such diverse activities remains poo ... Full text Link to item Cite

miR-133a regulates adipocyte browning in vivo.

Journal Article PLoS Genet · 2013 Prdm16 determines the bidirectional fate switch of skeletal muscle/brown adipose tissue (BAT) and regulates the thermogenic gene program of subcutaneous white adipose tissue (SAT) in mice. Here we show that miR-133a, a microRNA that is expressed in both BA ... Full text Link to item Cite

Plk1 phosphorylation of orc2 and hbo1 contributes to gemcitabine resistance in pancreatic cancer.

Journal Article Mol Cancer Ther · January 2013 Although gemcitabine is the standard chemotherapeutic drug for treatment of pancreatic cancer, almost all patients eventually develop resistance to this agent. Previous studies identified Polo-like kinase 1 (Plk1) as the mediator of gemcitabine resistance, ... Full text Link to item Cite

Fatty acid binding protein 4 expression marks a population of adipocyte progenitors in white and brown adipose tissues.

Journal Article FASEB J · January 2013 Adipose tissues regulate metabolism, reproduction, and life span. The development and growth of adipose tissue are due to increases of both adipocyte cell size and cell number; the latter is mediated by adipocyte progenitors. Various markers have been used ... Full text Link to item Cite

Reciprocal interaction between TRAF6 and notch signaling regulates adult myofiber regeneration upon injury.

Journal Article Mol Cell Biol · December 2012 Skeletal muscle is a postmitotic tissue that repairs and regenerates through activation of a population of stem-cell-like satellite cells. However, signaling mechanisms governing adult skeletal muscle regeneration remain less understood. In the present stu ... Full text Link to item Cite

Plk1 phosphorylates Sgt1 at the kinetochores to promote timely kinetochore-microtubule attachment.

Journal Article Mol Cell Biol · October 2012 Accurate chromosome segregation during cell division maintains genomic integrity and requires the proper establishment of kinetochore-microtubule attachment in mitosis. As a key regulator of mitosis, Polo-like kinase 1 (Plk1) is essential for this attachme ... Full text Link to item Cite

Myostatin facilitates slow and inhibits fast myosin heavy chain expression during myogenic differentiation.

Journal Article Biochem Biophys Res Commun · September 14, 2012 Skeletal muscles in the limb and body trunk are composed of heterogeneous myofibers expressing different isoforms of myosin heavy chain (Myh), including type I (slow, Myh7), IIA (intermediate, Myh2), IIX (fast, Myh1), and IIB (very fast, Myh4). While the c ... Full text Link to item Cite

Hypoxia promotes satellite cell self-renewal and enhances the efficiency of myoblast transplantation.

Journal Article Development · August 2012 Microenvironmental oxygen (O(2)) regulates stem cell activity, and a hypoxic niche with low oxygen levels has been reported in multiple stem cell types. Satellite cells are muscle-resident stem cells that maintain the homeostasis and mediate the regenerati ... Full text Link to item Cite

Constitutive Notch activation upregulates Pax7 and promotes the self-renewal of skeletal muscle satellite cells.

Journal Article Mol Cell Biol · June 2012 Notch signaling is a conserved cell fate regulator during development and postnatal tissue regeneration. Using skeletal muscle satellite cells as a model and through myogenic cell lineage-specific NICD(OE) (overexpression of constitutively activated Notch ... Full text Link to item Cite

Meat Science and Muscle Biology Symposium: stem cell niche and postnatal muscle growth.

Journal Article J Anim Sci · March 2012 Stem cell niche plays a critical role in regulating the behavior and function of adult stem cells that underlie tissue growth, maintenance, and regeneration. In the skeletal muscle, stem cells, called satellite cells, contribute to postnatal muscle growth ... Full text Link to item Cite

Mouse transgenic lines that selectively label Type I, Type IIA, and Types IIX+B skeletal muscle fibers.

Journal Article Genesis · January 2012 Skeletal muscle fibers vary in contractile and metabolic properties. Four main fiber types are present in mammalian trunk and limb muscles; they are called I, IIA, IIX, and IIB, ranging from slowest- to fastest-contracting. Individual muscles contain stere ... Full text Link to item Cite

TRIM32 regulates skeletal muscle stem cell differentiation and is necessary for normal adult muscle regeneration.

Journal Article PLoS One · 2012 Limb girdle muscular dystrophy type 2H (LGMD2H) is an inherited autosomal recessive disease of skeletal muscle caused by a mutation in the TRIM32 gene. Currently its pathogenesis is entirely unclear. Typically the regeneration process of adult skeletal mus ... Full text Link to item Cite

Intramuscular adipose is derived from a non-Pax3 lineage and required for efficient regeneration of skeletal muscles.

Journal Article Dev Biol · January 1, 2012 Ectopic accumulation of adipose in the skeletal muscle is associated with muscle wasting, insulin resistance and diabetes. However, the developmental origin of postnatal intramuscular adipose and its interaction with muscle tissue are unclear. We report he ... Full text Link to item Cite

Elevated levels of active matrix metalloproteinase-9 cause hypertrophy in skeletal muscle of normal and dystrophin-deficient mdx mice.

Journal Article Hum Mol Genet · November 15, 2011 Matrix metalloproteinases (MMPs) are a group of extracellular proteases involved in tissue remodeling in several physiological and pathophysiological conditions. While increased expression of MMPs (especially MMP-9) has been observed in skeletal muscle in ... Full text Link to item Cite

PPARδ regulates satellite cell proliferation and skeletal muscle regeneration.

Journal Article Skelet Muscle · November 1, 2011 Peroxisome proliferator-activated receptors (PPARs) are a class of nuclear receptors that play important roles in development and energy metabolism. Whereas PPARδ has been shown to regulate mitochondrial biosynthesis and slow-muscle fiber types, its functi ... Full text Link to item Cite

Identification and evolutionary implications of neurotransmitter-ciliary interactions underlying the behavioral response to hypoxia in Lymnaea stagnalis embryos.

Journal Article J Exp Biol · August 15, 2011 Acceleration of embryonic rotation is a common response to hypoxia among pond snails. It was first characterized in Helisoma trivolvis embryos, which have a pair of sensorimotor neurons that detect hypoxia and release serotonin onto postsynaptic ciliary ce ... Full text Link to item Cite

Peripheral endocannabinoids regulate skeletal muscle development and maintenance

Journal Article European Journal of Translational Myology · December 3, 2010 Full text Cite

Muscle stem cells

Chapter · December 1, 2010 Accumulating evidence suggests that there are two categories of stem cells in skeletal muscle: (1) satellite cells, the default muscle stem cells that are responsible for muscle growth and regeneration under physiological conditions and (2) other multipote ... Full text Cite

Dlk1 is necessary for proper skeletal muscle development and regeneration.

Journal Article PLoS One · November 29, 2010 Delta-like 1homolog (Dlk1) is an imprinted gene encoding a transmembrane protein whose increased expression has been associated with muscle hypertrophy in animal models. However, the mechanisms by which Dlk1 regulates skeletal muscle plasticity remain unkn ... Full text Link to item Cite

Genetic ablation of TWEAK augments regeneration and post-injury growth of skeletal muscle in mice.

Journal Article Am J Pathol · October 2010 Impairment in the regeneration process is a critical determinant for skeletal muscle wasting in chronic diseases and degenerative muscle disorders. Inflammatory cytokines are known to cause significant muscle wasting, however, their role in myofiber regene ... Full text Link to item Cite

p38-{gamma}-dependent gene silencing restricts entry into the myogenic differentiation program.

Journal Article J Cell Biol · December 28, 2009 The mitogen-activated protein kinase p38-gamma is highly expressed in skeletal muscle and is associated with the dystrophin glycoprotein complex; however, its function remains unclear. After induced damage, muscle in mice lacking p38-gamma generated signif ... Full text Link to item Cite

PRDM16 controls a brown fat/skeletal muscle switch.

Journal Article Nature · August 21, 2008 Brown fat can increase energy expenditure and protect against obesity through a specialized program of uncoupled respiration. Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previous ... Full text Link to item Cite

Integrative biology of an embryonic respiratory behaviour in pond snails: the 'embryo stir-bar hypothesis'.

Journal Article J Exp Biol · June 2008 Embryos of freshwater snails undergo direct development from single cell to juvenile inside egg masses that are deposited on vegetation and other substratum in pond, lake and stream habitats. Helisoma trivolvis, a member of the Planorbidae family of basomm ... Full text Link to item Cite

The emerging biology of satellite cells and their therapeutic potential.

Journal Article Trends Mol Med · February 2008 Adult skeletal muscle contains an abundant and highly accessible population of muscle stem and progenitor cells called satellite cells. The primary function of satellite cells is to mediate postnatal muscle growth and repair. Owing to their availability an ... Full text Link to item Cite

Niche regulation of muscle satellite cell self-renewal and differentiation.

Journal Article Cell Stem Cell · January 10, 2008 Muscle satellite cells have been shown to be a heterogeneous population of committed myogenic progenitors and noncommitted stem cells. This hierarchical composition of differentiating progenitors and self-renewable stem cells assures the extraordinary rege ... Full text Link to item Cite

The molecular regulation of muscle stem cell function.

Conference Cold Spring Harb Symp Quant Biol · 2008 Muscle satellite cells are responsible for the postnatal growth and robust regeneration capacity of adult skeletal muscle. A subset of satellite cells purified from adult skeletal muscle is capable of repopulating the satellite cell pool, suggesting that i ... Full text Link to item Cite

Megf10 regulates the progression of the satellite cell myogenic program.

Journal Article J Cell Biol · December 3, 2007 We identify here the multiple epidermal growth factor repeat transmembrane protein Megf10 as a quiescent satellite cell marker that is also expressed in skeletal myoblasts but not in differentiated myofibers. Retroviral expression of Megf10 in myoblasts re ... Full text Link to item Cite

Asymmetric self-renewal and commitment of satellite stem cells in muscle.

Journal Article Cell · June 1, 2007 Satellite cells play a central role in mediating the growth and regeneration of skeletal muscle. However, whether satellite cells are stem cells, committed progenitors, or dedifferentiated myoblasts has remained unclear. Using Myf5-Cre and ROSA26-YFP Cre-r ... Full text Link to item Cite

Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis.

Journal Article J Cell Biol · January 2, 2006 We assessed viable Pax7(-/-) mice in 129Sv/J background and observed reduced growth and marked muscle wasting together with a complete absence of functional satellite cells. Acute injury resulted in an extreme deficit in muscle regeneration. However, a sma ... Full text Link to item Cite

Ryanodine receptors in human pancreatic beta cells: localization and effects on insulin secretion.

Journal Article FASEB J · May 2004 It is clear that pancreatic beta-cell dysfunction, including basal hyperinsulinemia and reduced insulin release in response to glucose, is a key determinant of disease progression in type 2 diabetes, but the underlying molecular defects are not known. In d ... Full text Link to item Cite

Coordinated development of identified serotonergic neurons and their target ciliary cells in Helisoma trivolvis embryos.

Journal Article J Comp Neurol · March 17, 2003 Embryonic neuron C1s (ENC1s) are bilateral serotonergic neurons that function as cilioexcitatory motor neurons in embryonic development of the pond snail, Helisoma trivolvis. Recent experiments demonstrated that these neurons stimulate cilia-driven embryo ... Full text Link to item Cite

Long-term culture of decapsulated gastropod embryos: a transplantation study.

Journal Article Biol Bull · December 2002 Encapsulated embryos of the pond snail Helisoma trivolvis have been useful for examining neural development and neural circuit function during development. However, their full potential in developmental studies is limited by the lack of an effective method ... Full text Link to item Cite

Serotonergic sensory-motor neurons mediate a behavioral response to hypoxia in pond snail embryos.

Journal Article J Neurobiol · July 2002 Oxygen (O(2)) is one of the most important environmental factors that affects both physiological processes and development of aerobic animals, yet little is known about the neural mechanism of O(2) sensing and adaptive responses to low O(2) (hypoxia) durin ... Full text Link to item Cite

Laser ablation reveals regulation of ciliary activity by serotonergic neurons in molluscan embryos.

Journal Article J Neurobiol · April 2001 Early in embryonic development, the pond snail Helisoma trivolvis exhibits a rotational behavior that is generated by beating of cilia in the dorsolateral and pedal bands. Although previous anatomical and pharmacological studies provided indirect evidence ... Full text Link to item Cite

Seasonal studies of filtration rate and absorption efficiency in the scallop Chlamys farreri

Journal Article Journal of Shellfish Research · January 1, 1997 Seasonal studies of filtration rate, retention efficiency, and absorption efficiency in the native scallop Chlamys farreri, a major component of shellfisheries and aquaculture species in northern China since the 1970s, were carried out four times between S ... Cite