Thomas Greenfield Mitchell

Journal article J Clin Microbiol · January 2011 Featured Publication Compared to the incidence in adults, cryptococcosis is inexplicably rare among children, even in sub-Saharan Africa, which has the highest prevalence of coinfection with HIV and Cryptococcus neoformans. To explore any mycological basis for this age-related ... Full text Open Access Link to item Cite

Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.

Journal article Diagn Microbiol Infect Dis · May 2010 Featured Publication Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinica ... Full text Open Access Link to item Cite

Most environmental isolates of Cryptococcus neoformans var. grubii (serotype A) are not lethal for mice.

Journal article Infect Immun · August 2009 Featured Publication Most cases of cryptococcosis are caused by Cryptococcus neoformans var. grubii (serotype A), which is widespread in the environment, where it is primarily associated with pigeon excreta. A number of molecular epidemiological studies indicate that many envi ... Full text Link to item Cite

Many globally isolated AD hybrid strains of Cryptococcus neoformans originated in Africa.

Journal article PLoS Pathog · August 17, 2007 Featured Publication Interspecific and intervarietal hybridization may contribute to the biological diversity of fungal populations. Cryptococcus neoformans is a pathogenic yeast and the most common fungal cause of meningitis in patients with AIDS. Most patients are infected w ... Full text Link to item Cite

Multilocus sequence typing reveals three genetic subpopulations of Cryptococcus neoformans var. grubii (serotype A), including a unique population in Botswana.

Journal article Genetics · April 2006 Featured Publication We applied multilocus sequence typing (MLST) to investigate the population structure and mode of reproduction of Cryptococcus neoformans var. grubii (serotype A). This MLST system utilizes 12 unlinked polymorphic loci, which are dispersed on nine different ... Full text Link to item Cite

Comparative analysis of environmental and clinical populations of Cryptococcus neoformans.

Journal article J Clin Microbiol · February 2005 Featured Publication Cryptococcus neoformans is a major, global cause of meningoencephalitis in immunocompromised patients. Despite advances in the molecular epidemiology of C. neoformans, its population structure and mode of reproduction are not well understood. In the enviro ... Full text Link to item Cite

Phylogeny and evolution of medical species of Candida and related taxa: a multigenic analysis.

Journal article J Clin Microbiol · December 2004 Featured Publication Hemiascomycetes are species of yeasts within the order Saccharomycetales. The order encompasses disparate genera with a variety of life styles, including opportunistic human pathogens (e.g., Candida albicans), plant pathogens (e.g., Eremothecium gossypii), ... Full text Link to item Cite

A genetic linkage map of Cryptococcus neoformans variety neoformans serotype D (Filobasidiella neoformans).

Journal article Genetics · June 2004 Featured Publication To construct a genetic linkage map of the heterothallic yeast, Cryptococcus neoformans (Filobasidiella neoformans), we crossed two mating-compatible strains and analyzed 94 progeny for the segregation of 301 polymorphic markers, consisting of 228 restricti ... Full text Link to item Cite

Multiple gene genealogies reveal recent dispersion and hybridization in the human pathogenic fungus Cryptococcus neoformans.

Journal article Mol Ecol · October 2000 Featured Publication Cryptococcus neoformans (= Filobasidiella neoformans) is a significant emerging fungal pathogen of humans. To understand the evolution of this pathogen, 34 strains were obtained from various locations around the world and fragments of four genes were seque ... Full text Link to item Cite

Lack of genetic differentiation between two geographically diverse samples of Candida albicans isolated from patients infected with human immunodeficiency virus.

Journal article J Bacteriol · February 1999 Featured Publication The patterns of genetic variation of samples of Candida albicans isolated from patients infected with human immunodeficiency virus in Durham, N.C., and Vitória, Brazil, were compared. Genotypes for 126 strains were obtained at 16 polymorphic restriction si ... Full text Link to item Cite

Molecular markers reveal that population structure of the human pathogen Candida albicans exhibits both clonality and recombination.

Journal article Proc Natl Acad Sci U S A · October 29, 1996 Featured Publication The life history of Candida albicans presents an enigma: this species is thought to be exclusively asexual, yet strains show extensive phenotypic variation. To address the population genetics of C. albicans, we developed a genetic typing method for codomin ... Full text Link to item Cite

Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans.

Journal article Clin Microbiol Rev · October 1995 Featured Publication Although Cryptococcus neoformans and cryptococcosis have existed for several millennia, a century has passed since the discovery of this encapsulated yeast and its devastating disease. With the advent of the AIDS pandemic, cryptococcal meningitis has emerg ... Full text Link to item Cite

Unique oligonucleotide primers in PCR for identification of Cryptococcus neoformans.

Journal article J Clin Microbiol · January 1994 Featured Publication On the basis of a comparison of rRNA sequences coding the internal transcribed spacer (ITS) and 5.8S regions of Filobasidiella neoformans, the teleomorph of Cryptococcus neoformans, and members of its most closely related taxa, unique oligonucleotides were ... Full text Link to item Cite

Killing of Cryptococcus neoformans strains by human neutrophils and monocytes.

Journal article Infect Immun · January 1991 Featured Publication The susceptibility of various strains of Cryptococcus neoformans to killing by human polymorphonuclear leukocytes (PMNs) and monocytes was investigated. Five previously characterized strains of C. neoformans serotype A, a capsule-free mutant, and six recen ... Full text Link to item Cite