Scholars@Duke
Trevor Nolan

Trevor Nolan

Affiliate
Biology

Selected Publications

Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells.

Journal Article Cell · April 2025 Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis root, we reveal th ... Full text Cite

Two antagonistic gene regulatory networks drive Arabidopsis root hair growth at low temperature linked to a low-nutrient environment.

Journal Article The New phytologist · March 2025 Root hair (RH) cells can elongate to several hundred times their initial size, and are an ideal model system for investigating cell size control. Their development is influenced by both endogenous and external signals, which are combined to form an integra ... Full text Cite

Single-cell proteomics differentiates Arabidopsis root cell types.

Journal Article The New phytologist · December 2024 Single-cell proteomics (SCP) is an emerging approach to resolve cellular heterogeneity within complex tissues of multi-cellular organisms. Here, we demonstrate the feasibility of SCP on plant samples using the model plant Arabidopsis thaliana. Specifically ... Full text Cite

The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues.

Journal Article Science advances · September 2024 Brassinosteroid signaling is essential for plant growth as exemplified by the dwarf phenotype of loss-of-function mutants in BRASSINOSTEROID INSENSITIVE 1 (BRI1), a ubiquitously expressed Arabidopsis brassinosteroid receptor gene. Complementa ... Full text Cite

Plasmodesmata mediate cell-to-cell transport of brassinosteroid hormones.

Journal Article Nature chemical biology · November 2023 Brassinosteroids (BRs) are steroidal phytohormones that are essential for plant growth, development and adaptation to environmental stresses. BRs act in a dose-dependent manner and do not travel over long distances; hence, BR homeostasis maintenance is cri ... Full text Cite

Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root.

Journal Article Science (New York, N.Y.) · March 2023 Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid ... Full text Cite

Integration of multi-omics data reveals interplay between brassinosteroid and Target of Rapamycin Complex signaling in Arabidopsis.

Journal Article The New phytologist · November 2022 Brassinosteroids (BRs) and Target of Rapamycin Complex (TORC) are two major actors coordinating plant growth and stress responses. Brassinosteroids function through a signaling pathway to extensively regulate gene expression and TORC is known to regulate t ... Full text Cite

Integrated omics reveal novel functions and underlying mechanisms of the receptor kinase FERONIA in Arabidopsis thaliana.

Journal Article The Plant cell · July 2022 The receptor kinase FERONIA (FER) is a versatile regulator of plant growth and development, biotic and abiotic stress responses, and reproduction. To gain new insights into the molecular interplay of these processes and to identify new FER functions, we ca ... Full text Cite

The F-box E3 ubiquitin ligase BAF1 mediates the degradation of the brassinosteroid-activated transcription factor BES1 through selective autophagy in Arabidopsis.

Journal Article The Plant cell · November 2021 Brassinosteroids (BRs) regulate plant growth, development, and stress responses by activating the core transcription factor BRI1-EMS-SUPPRESSOR1 (BES1), whose degradation occurs through the proteasome and autophagy pathways. The E3 ubiquitin ligase(s) that ... Full text Cite

Single-cell analysis of cell identity in the Arabidopsis root apical meristem: insights and opportunities.

Journal Article Journal of experimental botany · October 2021 A fundamental question in developmental biology is how the progeny of stem cells become differentiated tissues. The Arabidopsis root is a tractable model to address this question due to its simple organization and defined cell lineages. In particular, the ... Full text Cite

Integrated omics networks reveal the temporal signaling events of brassinosteroid response in Arabidopsis.

Journal Article Nature communications · October 2021 Brassinosteroids (BRs) are plant steroid hormones that regulate cell division and stress response. Here we use a systems biology approach to integrate multi-omic datasets and unravel the molecular signaling events of BR response in Arabidopsis. We profile ... Full text Cite

Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses.

Journal Article The Plant cell · February 2020 Brassinosteroids (BRs) are a group of polyhydroxylated plant steroid hormones that are crucial for many aspects of a plant's life. BRs were originally characterized for their function in cell elongation, but it is becoming clear that they play major roles ... Full text Cite

A single cellArabidopsisroot atlas reveals developmental trajectories in wild type and cell identity mutants

Journal Article · 2020 Summary Cell fate acquisition is a fundamental developmental process in all multicellular organisms. Yet, much is unknown regarding how a cell traverses the pathway from stem cell to terminal differentiation. Advances in single cell genomics 1 hol ... Full text Cite

Identification of transcription factors that regulate ATG8 expression and autophagy in Arabidopsis.

Journal Article Autophagy · January 2020 Autophagy is a conserved catabolic process in eukaryotes that contributes to cell survival in response to multiple stresses and is important for organism fitness. In Arabidopsis thaliana, the core machinery of autophagy is well defined, but its tran ... Full text Cite

The AP2/ERF Transcription Factor TINY Modulates Brassinosteroid-Regulated Plant Growth and Drought Responses in Arabidopsis.

Journal Article The Plant cell · August 2019 APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) family transcription factors have well-documented functions in stress responses, but their roles in brassinosteroid (BR)-regulated growth and stress responses have not been established. Here, we show that the A ... Full text Cite

AP2/ERF Transcription Factor Regulatory Networks in Hormone and Abiotic Stress Responses in Arabidopsis.

Journal Article Frontiers in plant science · January 2019 Dynamic environmental changes such as extreme temperature, water scarcity and high salinity affect plant growth, survival, and reproduction. Plants have evolved sophisticated regulatory mechanisms to adapt to these unfavorable conditions, many of which int ... Full text Cite

FERONIA Receptor Kinase Contributes to Plant Immunity by Suppressing Jasmonic Acid Signaling in Arabidopsis thaliana.

Journal Article Current biology : CB · October 2018 Bacterial pathogens use effectors and phytotoxins to facilitate infection of host plants. Coronatine (COR) is one of the phytotoxins produced in bacterial pathogens, such as Pseudomonas syringae pv. tomato DC3000 (pst DC3000). COR structurally and function ... Full text Cite

Automated microfluidic plant chips-based plant phenotyping system

Conference 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 · August 25, 2017 We report on the development of an enhanced and robust microfluidic plant chip-based growth and monitoring system for high-Throughput phenotyping of Arabidopsis thaliana. The system consists of multiple vertical plant chips, a microfluidic hormone concentr ... Full text Cite

Cross-talk of Brassinosteroid signaling in controlling growth and stress responses.

Journal Article The Biochemical journal · July 2017 Plants are faced with a barrage of stresses in their environment and must constantly balance their growth and survival. As such, plants have evolved complex control systems that perceive and respond to external and internal stimuli in order to optimize the ... Full text Cite

Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses.

Journal Article The Plant cell · June 2017 Plant steroid hormones, brassinosteroids (BRs), play important roles in growth and development. BR signaling controls the activities of BRASSINOSTERIOD INSENSITIVE1-EMS-SUPPRESSOR1/BRASSINAZOLE-RESISTANT1 (BES1/BZR1) family transcription factors. Besides t ... Full text Cite

Selective Autophagy of BES1 Mediated by DSK2 Balances Plant Growth and Survival.

Journal Article Developmental cell · April 2017 Plants encounter a variety of stresses and must fine-tune their growth and stress-response programs to best suit their environment. BES1 functions as a master regulator in the brassinosteroid (BR) pathway that promotes plant growth. Here, we show that BES1 ... Full text Cite

SINAT E3 Ligases Control the Light-Mediated Stability of the Brassinosteroid-Activated Transcription Factor BES1 in Arabidopsis.

Journal Article Developmental cell · April 2017 The plant hormones brassinosteroids (BRs) participate in light-mediated regulation of plant growth, although the underlying mechanisms are far from being fully understood. In addition, the function of the core transcription factor in the BR signaling pathw ... Full text Cite

RD26 mediates crosstalk between drought and brassinosteroid signalling pathways.

Journal Article Nature communications · February 2017 Brassinosteroids (BRs) regulate plant growth and stress responses via the BES1/BZR1 family of transcription factors, which regulate the expression of thousands of downstream genes. BRs are involved in the response to drought, however the mechanistic unders ... Full text Cite

Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing.

Chapter · January 2017 Brassinosteroids (BRs) play important roles in many growth and developmental processes. BRs signal to regulate BR-INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulat ... Full text Cite

Impaired Chloroplast Biogenesis in Immutans, an Arabidopsis Variegation Mutant, Modifies Developmental Programming, Cell Wall Composition and Resistance to Pseudomonas syringae.

Journal Article PloS one · January 2016 The immutans (im) variegation mutation of Arabidopsis has green- and white- sectored leaves due to action of a nuclear recessive gene. IM codes for PTOX, a plastoquinol oxidase in plastid membranes. Previous studies have revealed that the green and white s ... Full text Cite

Histone lysine methyltransferase SDG8 is involved in brassinosteroid-regulated gene expression in Arabidopsis thaliana.

Journal Article Molecular plant · August 2014 The plant steroid hormones, brassinosteroids (BRs), play important roles in plant growth, development, and responses to environmental stresses. BRs signal through receptors localized to the plasma membrane and other signaling components to regulate the BES ... Full text Cite

Understanding chloroplast biogenesis using second-site suppressors of immutans and var2.

Journal Article Photosynthesis research · October 2013 Chloroplast biogenesis is an essential light-dependent process involving the differentiation of photosynthetically competent chloroplasts from precursors that include undifferentiated proplastids in leaf meristems, as well as etioplasts in dark-grown seedl ... Full text Cite

The Mechanism of Variegation in immutans Provides Insight into Chloroplast Biogenesis.

Journal Article Frontiers in plant science · January 2012 The immutans (im) variegation mutant of Arabidopsis has green and white-sectored leaves due to the absence of fully functional plastid terminal oxidase (PTOX), a plastoquinol oxidase in thylakoid membranes. PTOX appears to be at the nexus of a growing numb ... Full text Cite