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ERAAP synergizes with MHC class I molecules to make the final cut in the antigenic peptide precursors in the endoplasmic reticulum.

Publication ,  Journal Article
Kanaseki, T; Blanchard, N; Hammer, GE; Gonzalez, F; Shastri, N
Published in: Immunity
November 2006

The major histocompatibility complex class I molecules display peptides (pMHC I) on the cell surface for immune surveillance by CD8(+) T cells. These peptides are generated by proteolysis of intracellular polypeptides by the proteasome in the cytoplasm and then in the endoplasmic reticulum (ER) by the ER aminopeptidase associated with antigen processing (ERAAP). To define the unknown mechanism of ERAAP function in vivo, we analyzed naturally processed peptides in cells with or without appropriate MHC I and ERAAP. In the absence of MHC I, ERAAP degraded the antigenic precursors in the ER. However, MHC I molecules could bind proteolytic intermediates and were essential for generation of the final peptide by ERAAP. Thus, ERAAP synergizes with MHC I to generate the final pMHC I repertoire.

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Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

November 2006

Volume

25

Issue

5

Start / End Page

795 / 806

Location

United States

Related Subject Headings

  • Transfection
  • Polymerase Chain Reaction
  • Peptides
  • Molecular Sequence Data
  • Mice
  • Lymphocyte Activation
  • Leucyl Aminopeptidase
  • Immunology
  • Immunohistochemistry
  • Immunoblotting
 

Citation

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Chicago
ICMJE
MLA
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Kanaseki, T., Blanchard, N., Hammer, G. E., Gonzalez, F., & Shastri, N. (2006). ERAAP synergizes with MHC class I molecules to make the final cut in the antigenic peptide precursors in the endoplasmic reticulum. Immunity, 25(5), 795–806. https://doi.org/10.1016/j.immuni.2006.09.012
Kanaseki, Takayuki, Nicolas Blanchard, Gianna Elena Hammer, Federico Gonzalez, and Nilabh Shastri. “ERAAP synergizes with MHC class I molecules to make the final cut in the antigenic peptide precursors in the endoplasmic reticulum.Immunity 25, no. 5 (November 2006): 795–806. https://doi.org/10.1016/j.immuni.2006.09.012.
Kanaseki T, Blanchard N, Hammer GE, Gonzalez F, Shastri N. ERAAP synergizes with MHC class I molecules to make the final cut in the antigenic peptide precursors in the endoplasmic reticulum. Immunity. 2006 Nov;25(5):795–806.
Kanaseki, Takayuki, et al. “ERAAP synergizes with MHC class I molecules to make the final cut in the antigenic peptide precursors in the endoplasmic reticulum.Immunity, vol. 25, no. 5, Nov. 2006, pp. 795–806. Pubmed, doi:10.1016/j.immuni.2006.09.012.
Kanaseki T, Blanchard N, Hammer GE, Gonzalez F, Shastri N. ERAAP synergizes with MHC class I molecules to make the final cut in the antigenic peptide precursors in the endoplasmic reticulum. Immunity. 2006 Nov;25(5):795–806.
Journal cover image

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

November 2006

Volume

25

Issue

5

Start / End Page

795 / 806

Location

United States

Related Subject Headings

  • Transfection
  • Polymerase Chain Reaction
  • Peptides
  • Molecular Sequence Data
  • Mice
  • Lymphocyte Activation
  • Leucyl Aminopeptidase
  • Immunology
  • Immunohistochemistry
  • Immunoblotting