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Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes.

Publication ,  Journal Article
Van Wettere, AJ; Law, JM; Hinton, DE; Kullman, SW
Published in: Toxicologic pathology
January 2014

Transgenic organisms that express fluorescent proteins are used frequently for in vivo visualization of proteins and cells. The phenotype of a transgenic medaka (Oryzias latipes) strain that expresses a red fluorescent protein (RFP) in hepatocytes was characterized using light and fluorescence microscopy, immunohistochemistry, and transmission electron microscopy (TEM). Expression of RFP was first detected by confocal fluorescence microscopy in the location of the liver bud of live medaka embryos at 60 hr postfertilization (developmental stage 27). Subsequently, RFP signal was observed exclusively in hepatocytes throughout life using fluorescence microscopy in live fish and immunohistochemistry in formalin-fixed, paraffin-embedded liver sections. As the fish aged, prominent intracytoplasmic eosinophilic inclusions immunoreactive for RFP were observed by light microscopy and were correlated with membrane-bound electron dense inclusions on TEM. These results define the onset and location of RFP expression in the Tg(zf.L-fabp:DsRed) medaka and characterize a histologic phenotype that results from RFP expression in hepatocytes.

Duke Scholars

Published In

Toxicologic pathology

DOI

EISSN

1533-1601

ISSN

0192-6233

Publication Date

January 2014

Volume

42

Issue

3

Start / End Page

616 / 621

Related Subject Headings

  • Toxicology
  • Red Fluorescent Protein
  • Phenotype
  • Oryzias
  • Male
  • Luminescent Proteins
  • Liver
  • Hepatocytes
  • Female
  • Fatty Acid-Binding Proteins
 

Citation

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ICMJE
MLA
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Van Wettere, A. J., Law, J. M., Hinton, D. E., & Kullman, S. W. (2014). Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes. Toxicologic Pathology, 42(3), 616–621. https://doi.org/10.1177/0192623313499643
Van Wettere, Arnaud J., J Mac Law, David E. Hinton, and Seth W. Kullman. “Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes.Toxicologic Pathology 42, no. 3 (January 2014): 616–21. https://doi.org/10.1177/0192623313499643.
Van Wettere AJ, Law JM, Hinton DE, Kullman SW. Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes. Toxicologic pathology. 2014 Jan;42(3):616–21.
Van Wettere, Arnaud J., et al. “Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes.Toxicologic Pathology, vol. 42, no. 3, Jan. 2014, pp. 616–21. Epmc, doi:10.1177/0192623313499643.
Van Wettere AJ, Law JM, Hinton DE, Kullman SW. Phenotypic characterization of transgenic Japanese medaka (Oryzias latipes) that express a red fluorescent protein in hepatocytes. Toxicologic pathology. 2014 Jan;42(3):616–621.
Journal cover image

Published In

Toxicologic pathology

DOI

EISSN

1533-1601

ISSN

0192-6233

Publication Date

January 2014

Volume

42

Issue

3

Start / End Page

616 / 621

Related Subject Headings

  • Toxicology
  • Red Fluorescent Protein
  • Phenotype
  • Oryzias
  • Male
  • Luminescent Proteins
  • Liver
  • Hepatocytes
  • Female
  • Fatty Acid-Binding Proteins