Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.

Publication ,  Journal Article
Lindy, AS; Parekh, PK; Zhu, R; Kanju, P; Chintapalli, SV; Tsvilovskyy, V; Patterson, RL; Anishkin, A; van Rossum, DB; Liedtke, WB
Published in: Nat Commun
September 2, 2014

Animals need to sense and react to potentially dangerous environments. TRP ion channels participate in nociception, presumably via Ca(2+) influx, in most animal species. However, the relationship between ion permeation and animals' nocifensive behaviour is unknown. Here we use an invertebrate animal model with relevance for mammalian pain. We analyse the putative selectivity filter of OSM-9, a TRPV channel, in osmotic avoidance behaviour of Caenorhabditis elegans. Using mutagenized OSM-9 expressed in the head nociceptor neuron, ASH, we study nocifensive behaviour and Ca(2+) influx. Within the selectivity filter, M(601)-F(609), Y604G strongly reduces avoidance behaviour and eliminates Ca(2+) transients. Y604F also abolishes Ca(2+) transients in ASH, while sustaining avoidance behaviour, yet it disrupts behavioral plasticity. Homology modelling of the OSM-9 pore suggests that Y(604) may assume a scaffolding role. Thus, aromatic residues in the OSM-9 selectivity filter are critical for pain behaviour and ion permeation. These findings have relevance for understanding evolutionary roots of mammalian nociception.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

September 2, 2014

Volume

5

Start / End Page

4734

Location

England

Related Subject Headings

  • TRPV Cation Channels
  • Structural Homology, Protein
  • Sequence Alignment
  • Recombinant Fusion Proteins
  • Nociceptors
  • Nociception
  • Nerve Tissue Proteins
  • Mutation
  • Molecular Sequence Data
  • Models, Molecular
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lindy, A. S., Parekh, P. K., Zhu, R., Kanju, P., Chintapalli, S. V., Tsvilovskyy, V., … Liedtke, W. B. (2014). TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour. Nat Commun, 5, 4734. https://doi.org/10.1038/ncomms5734
Lindy, Amanda S., Puja K. Parekh, Richard Zhu, Patrick Kanju, Sree V. Chintapalli, Volodymyr Tsvilovskyy, Randen L. Patterson, Andriy Anishkin, Damian B. van Rossum, and Wolfgang B. Liedtke. “TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.Nat Commun 5 (September 2, 2014): 4734. https://doi.org/10.1038/ncomms5734.
Lindy AS, Parekh PK, Zhu R, Kanju P, Chintapalli SV, Tsvilovskyy V, et al. TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour. Nat Commun. 2014 Sep 2;5:4734.
Lindy, Amanda S., et al. “TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.Nat Commun, vol. 5, Sept. 2014, p. 4734. Pubmed, doi:10.1038/ncomms5734.
Lindy AS, Parekh PK, Zhu R, Kanju P, Chintapalli SV, Tsvilovskyy V, Patterson RL, Anishkin A, van Rossum DB, Liedtke WB. TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour. Nat Commun. 2014 Sep 2;5:4734.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

September 2, 2014

Volume

5

Start / End Page

4734

Location

England

Related Subject Headings

  • TRPV Cation Channels
  • Structural Homology, Protein
  • Sequence Alignment
  • Recombinant Fusion Proteins
  • Nociceptors
  • Nociception
  • Nerve Tissue Proteins
  • Mutation
  • Molecular Sequence Data
  • Models, Molecular