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Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.

Publication ,  Journal Article
Hsu, C-W; Poché, RA; Saik, JE; Ali, S; Wang, S; Yosef, N; Calderon, GA; Scott, L; Vadakkan, TJ; Larina, IV; West, JL; Dickinson, ME
Published in: PloS one
January 2015

Successful engineering of complex organs requires improved methods to promote rapid and stable vascularization of artificial tissue scaffolds. Toward this goal, tissue engineering strategies utilize the release of pro-angiogenic growth factors, alone or in combination, from biomaterials to induce angiogenesis. In this study we have used intravital microscopy to define key, dynamic cellular changes induced by the release of pro-angiogenic factors from polyethylene glycol diacrylate hydrogels transplanted in vivo. Our data show robust macrophage recruitment when the potent and synergistic angiogenic factors, PDGFBB and FGF2 were used as compared with VEGF alone and intravital imaging suggested roles for macrophages in endothelial tip cell migration and anastomosis, as well as pericyte-like behavior. Further data from in vivo experiments show that delivery of CSF1 with VEGF can dramatically improve the poor angiogenic response seen with VEGF alone. These studies show that incorporating macrophage-recruiting factors into the design of pro-angiogenic biomaterial scaffolds is a key strategy likely to be necessary for stable vascularization and survival of implanted artificial tissues.

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Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2015

Volume

10

Issue

7

Start / End Page

e0131643

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tissue Scaffolds
  • Tissue Engineering
  • Proto-Oncogene Proteins c-sis
  • Neovascularization, Physiologic
  • Mice
  • Macrophages
  • Hydrogels
  • Humans
  • Human Umbilical Vein Endothelial Cells
 

Citation

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Hsu, C.-W., Poché, R. A., Saik, J. E., Ali, S., Wang, S., Yosef, N., … Dickinson, M. E. (2015). Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules. PloS One, 10(7), e0131643. https://doi.org/10.1371/journal.pone.0131643
Hsu, Chih-Wei, Ross A. Poché, Jennifer E. Saik, Saniya Ali, Shang Wang, Nejla Yosef, Gisele A. Calderon, et al. “Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.PloS One 10, no. 7 (January 2015): e0131643. https://doi.org/10.1371/journal.pone.0131643.
Hsu C-W, Poché RA, Saik JE, Ali S, Wang S, Yosef N, et al. Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules. PloS one. 2015 Jan;10(7):e0131643.
Hsu, Chih-Wei, et al. “Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.PloS One, vol. 10, no. 7, Jan. 2015, p. e0131643. Epmc, doi:10.1371/journal.pone.0131643.
Hsu C-W, Poché RA, Saik JE, Ali S, Wang S, Yosef N, Calderon GA, Scott L, Vadakkan TJ, Larina IV, West JL, Dickinson ME. Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules. PloS one. 2015 Jan;10(7):e0131643.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2015

Volume

10

Issue

7

Start / End Page

e0131643

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tissue Scaffolds
  • Tissue Engineering
  • Proto-Oncogene Proteins c-sis
  • Neovascularization, Physiologic
  • Mice
  • Macrophages
  • Hydrogels
  • Humans
  • Human Umbilical Vein Endothelial Cells