Skip to main content

Tumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markers.

Publication ,  Journal Article
Karathanasis, E; Chan, L; Karumbaiah, L; McNeeley, K; D'Orsi, CJ; Annapragada, AV; Sechopoulos, I; Bellamkonda, RV
Published in: PloS one
June 2009

Vascular endothelial growth factor (VEGF) receptor-2 is the major mediator of the mitogenic, angiogenic, and vascular hyperpermeability effects of VEGF on breast tumors. Overexpression of VEGF and VEGF receptor-2 is associated with the degree of pathomorphosis of the tumor tissue and unfavorable prognosis. In this study, we demonstrate that non-invasive quantification of the degree of tumor vascular permeability to a nanoprobe correlates with the VEGF and its receptor levels and tumor growth.We designed an imaging nanoprobe and a methodology to detect the intratumoral deposition of a 100 nm-scale nanoprobe using mammography allowing measurement of the tumor vascular permeability in a rat MAT B III breast tumor model. The tumor vascular permeability varied widely among the animals. Notably, the VEGF and VEGF receptor-2 gene expression of the tumors as measured by qRT-PCR displayed a strong correlation to the imaging-based measurements of vascular permeability to the 100 nm-scale nanoprobe. This is in good agreement with the fact that tumors with high angiogenic activity are expected to have more permeable blood vessels resulting in high intratumoral deposition of a nanoscale agent. In addition, we show that higher intratumoral deposition of the nanoprobe as imaged with mammography correlated to a faster tumor growth rate. This data suggest that vascular permeability scales to the tumor growth and that tumor vascular permeability can be a measure of underlying VEGF and VEGF receptor-2 expression in individual tumors.This is the first demonstration, to our knowledge, that quantitative imaging of tumor vascular permeability to a nanoprobe represents a form of a surrogate, functional biomarker of underlying molecular markers of angiogenesis.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

June 2009

Volume

4

Issue

6

Start / End Page

e5843

Related Subject Headings

  • X-Rays
  • Vascular Endothelial Growth Factor Receptor-2
  • Vascular Endothelial Growth Factor A
  • Signal Transduction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats
  • Neovascularization, Pathologic
  • Neoplasms
  • Nanotechnology
  • Mammary Neoplasms, Animal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Karathanasis, E., Chan, L., Karumbaiah, L., McNeeley, K., D’Orsi, C. J., Annapragada, A. V., … Bellamkonda, R. V. (2009). Tumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markers. PloS One, 4(6), e5843. https://doi.org/10.1371/journal.pone.0005843
Karathanasis, Efstathios, Leslie Chan, Lohitash Karumbaiah, Kathleen McNeeley, Carl J. D’Orsi, Ananth V. Annapragada, Ioannis Sechopoulos, and Ravi V. Bellamkonda. “Tumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markers.PloS One 4, no. 6 (June 2009): e5843. https://doi.org/10.1371/journal.pone.0005843.
Karathanasis E, Chan L, Karumbaiah L, McNeeley K, D’Orsi CJ, Annapragada AV, et al. Tumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markers. PloS one. 2009 Jun;4(6):e5843.
Karathanasis, Efstathios, et al. “Tumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markers.PloS One, vol. 4, no. 6, June 2009, p. e5843. Epmc, doi:10.1371/journal.pone.0005843.
Karathanasis E, Chan L, Karumbaiah L, McNeeley K, D’Orsi CJ, Annapragada AV, Sechopoulos I, Bellamkonda RV. Tumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markers. PloS one. 2009 Jun;4(6):e5843.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

June 2009

Volume

4

Issue

6

Start / End Page

e5843

Related Subject Headings

  • X-Rays
  • Vascular Endothelial Growth Factor Receptor-2
  • Vascular Endothelial Growth Factor A
  • Signal Transduction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rats
  • Neovascularization, Pathologic
  • Neoplasms
  • Nanotechnology
  • Mammary Neoplasms, Animal