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eNOS-overexpressing endothelial cells inhibit platelet aggregation and smooth muscle cell proliferation in vitro.

Publication ,  Journal Article
Kader, KN; Akella, R; Ziats, NP; Lakey, LA; Harasaki, H; Ranieri, JP; Bellamkonda, RV
Published in: Tissue engineering
June 2000

Endothelial cell seeding of synthetic small diameter vascular grafts (SSDVG) has been shown to diminish thrombosis and intimal hyperplasia, resulting in improved graft patency. However, endothelial cell retention on seeded grafts when exposed to physiological shearing conditions remains poor. We report that the genetic engineering of endothelial cells to overexpress endothelial nitric oxide synthase (eNOS), may create improved anti-thrombotic and anti-hyperplastic endothelial cell phenotypes for SSDVG seeding. eNOS-overexpressing endothelial cells may potentially overcome the biochemical loss due to shear induced reduction in endothelial cell coverage on SSDVG. Bovine aortic endothelial cells (BAEC) were transfected with the human eNOS gene, and co-incubated with either human whole blood or bovine aortic smooth muscle cells (BASMC) in vitro. eNOS-transfected BAEC significantly overexpressed eNOS compared to control beta-Gal-transfected and untransfected BAEC up to 120 h post transfection. In co-incubation and co-culture assays, human platelet aggregation decreased by 46% and BASMC proliferation decreased by 67.2% when compared to incubation with untransfected BAEC.

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Published In

Tissue engineering

DOI

EISSN

1557-8690

ISSN

1076-3279

Publication Date

June 2000

Volume

6

Issue

3

Start / End Page

241 / 251

Related Subject Headings

  • Platelet Aggregation
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase
  • Muscle, Smooth, Vascular
  • Humans
  • Genetic Engineering
  • Endothelium, Vascular
  • Coculture Techniques
  • Cells, Cultured
  • Cell Division
 

Citation

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Kader, K. N., Akella, R., Ziats, N. P., Lakey, L. A., Harasaki, H., Ranieri, J. P., & Bellamkonda, R. V. (2000). eNOS-overexpressing endothelial cells inhibit platelet aggregation and smooth muscle cell proliferation in vitro. Tissue Engineering, 6(3), 241–251. https://doi.org/10.1089/10763270050044425
Kader, K. N., R. Akella, N. P. Ziats, L. A. Lakey, H. Harasaki, J. P. Ranieri, and R. V. Bellamkonda. “eNOS-overexpressing endothelial cells inhibit platelet aggregation and smooth muscle cell proliferation in vitro.Tissue Engineering 6, no. 3 (June 2000): 241–51. https://doi.org/10.1089/10763270050044425.
Kader KN, Akella R, Ziats NP, Lakey LA, Harasaki H, Ranieri JP, et al. eNOS-overexpressing endothelial cells inhibit platelet aggregation and smooth muscle cell proliferation in vitro. Tissue engineering. 2000 Jun;6(3):241–51.
Kader, K. N., et al. “eNOS-overexpressing endothelial cells inhibit platelet aggregation and smooth muscle cell proliferation in vitro.Tissue Engineering, vol. 6, no. 3, June 2000, pp. 241–51. Epmc, doi:10.1089/10763270050044425.
Kader KN, Akella R, Ziats NP, Lakey LA, Harasaki H, Ranieri JP, Bellamkonda RV. eNOS-overexpressing endothelial cells inhibit platelet aggregation and smooth muscle cell proliferation in vitro. Tissue engineering. 2000 Jun;6(3):241–251.

Published In

Tissue engineering

DOI

EISSN

1557-8690

ISSN

1076-3279

Publication Date

June 2000

Volume

6

Issue

3

Start / End Page

241 / 251

Related Subject Headings

  • Platelet Aggregation
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase
  • Muscle, Smooth, Vascular
  • Humans
  • Genetic Engineering
  • Endothelium, Vascular
  • Coculture Techniques
  • Cells, Cultured
  • Cell Division