Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Monoclonal antibodies that detect biochemical markers of arthritis in humans.

Publication ,  Journal Article
Slater, RR; Bayliss, MT; Lachiewicz, PF; Visco, DM; Caterson, B
Published in: Arthritis Rheum
May 1995

OBJECTIVE: To evaluate the potential of using monoclonal antibodies (MAb) 3-B-3(-) and 7-D-4 to detect biochemical markers of altered cartilage metabolism in human arthritides. METHODS: Fifty-five samples of normal articular cartilage (subjects' age range 18 weeks of gestation to 83 years of age) and 89 samples of arthritic cartilage (patients' age range 20-81 years) were collected, and their proteoglycans were extracted and analyzed for the presence of the epitopes recognized by MAb 3-B-3 and 7-D-4. RESULTS: Native 3-B-3(-) mimotope was expressed at a high incidence in proteoglycans extracted from the cartilage of patients with most of the arthritic diseases examined (osteoarthritis, juvenile rheumatoid arthritis, rheumatoid arthritis, avascular necrosis, and degenerative meniscal tears). Its expression in normal cartilage specimens was very low or absent, occurring mainly in the young, skeletally immature individuals. In contrast, expression of the 7-D-4 epitope was more variable in patients with different arthritides and was also frequently found in normal cartilage specimens. Immunohistochemical analyses with both 3-B-3(-) and 7-D-4 showed strong focal positive staining in superficial areas, where cartilage degeneration, remodeling, and repair were greatest. CONCLUSION: The biochemical markers recognized by MAb 3-B-3(-) and 7-D-4 are indicative of altered proteoglycan synthesis and metabolism in human articular cartilage. The data suggest that in human cartilage, the 3-B-3(-) epitope might be a better marker of biochemical changes than the 7-D-4 epitope.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

May 1995

Volume

38

Issue

5

Start / End Page

655 / 659

Location

United States

Related Subject Headings

  • Proteoglycans
  • Osteoarthritis
  • Middle Aged
  • Infant, Newborn
  • Infant
  • Humans
  • Epitopes
  • Child, Preschool
  • Child
  • Cartilage, Articular
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Slater, R. R., Bayliss, M. T., Lachiewicz, P. F., Visco, D. M., & Caterson, B. (1995). Monoclonal antibodies that detect biochemical markers of arthritis in humans. Arthritis Rheum, 38(5), 655–659. https://doi.org/10.1002/art.1780380513
Slater, R. R., M. T. Bayliss, P. F. Lachiewicz, D. M. Visco, and B. Caterson. “Monoclonal antibodies that detect biochemical markers of arthritis in humans.Arthritis Rheum 38, no. 5 (May 1995): 655–59. https://doi.org/10.1002/art.1780380513.
Slater RR, Bayliss MT, Lachiewicz PF, Visco DM, Caterson B. Monoclonal antibodies that detect biochemical markers of arthritis in humans. Arthritis Rheum. 1995 May;38(5):655–9.
Slater, R. R., et al. “Monoclonal antibodies that detect biochemical markers of arthritis in humans.Arthritis Rheum, vol. 38, no. 5, May 1995, pp. 655–59. Pubmed, doi:10.1002/art.1780380513.
Slater RR, Bayliss MT, Lachiewicz PF, Visco DM, Caterson B. Monoclonal antibodies that detect biochemical markers of arthritis in humans. Arthritis Rheum. 1995 May;38(5):655–659.
Journal cover image

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

May 1995

Volume

38

Issue

5

Start / End Page

655 / 659

Location

United States

Related Subject Headings

  • Proteoglycans
  • Osteoarthritis
  • Middle Aged
  • Infant, Newborn
  • Infant
  • Humans
  • Epitopes
  • Child, Preschool
  • Child
  • Cartilage, Articular