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Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion.

Publication ,  Journal Article
Hasegawa, K; Zhang, P; Wei, Z; Pomeroy, JE; Lu, W; Pera, MF
Published in: Stem Cells
August 2010

Reprogramming human somatic cells into pluripotent cells opens up new possibilities for transplantation therapy, the study of disease, and drug screening. In addition to somatic cell nuclear transfer, several approaches to reprogramming human cells have been reported: transduction of defined transcription factors to generate induced pluripotent stem cell (iPSC), human embryonic stem cell (hESC)-somatic cell fusion, and hESC cytoplast-somatic cell fusion or exposure to extracts of hESC. Here, we optimized techniques for hESC-human fibroblast fusion and enucleation and cytoplast fusion, and then compared the reprogramming efficiency between iPSC generation, cell-fusion and cytoplast-fusion. When compared with iPSC, hESC-fusion provided much faster and efficient reprogramming of somatic cells. The reprogramming required more than 4 weeks and the efficiency was less than 0.001% in iPSC generation, and it was less than 10 days and more than 0.005% in hESC-fusion. In addition, fusion yielded almost no partially reprogrammed cell colonies. However, the fused cells were tetraploid or aneuploid. hESC cytoplast fusion could initiate reprogramming but was never able to complete reprogramming. These data indicate that in cell fusion, as in nuclear transfer, reprogramming through direct introduction of a somatic nucleus into the environment of a pluripotent cell provides relatively efficient reprogramming. The findings also suggest that the nucleus of the host pluripotent cell may contain components that accelerate the reprogramming process.

Duke Scholars

Published In

Stem Cells

DOI

EISSN

1549-4918

Publication Date

August 2010

Volume

28

Issue

8

Start / End Page

1338 / 1348

Location

England

Related Subject Headings

  • Transduction, Genetic
  • SOXB1 Transcription Factors
  • Proto-Oncogene Proteins c-myc
  • Octamer Transcription Factor-3
  • Lentivirus
  • Kruppel-Like Transcription Factors
  • Kruppel-Like Factor 4
  • Induced Pluripotent Stem Cells
  • Immunology
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hasegawa, K., Zhang, P., Wei, Z., Pomeroy, J. E., Lu, W., & Pera, M. F. (2010). Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion. Stem Cells, 28(8), 1338–1348. https://doi.org/10.1002/stem.466
Hasegawa, Kouichi, Peilin Zhang, Zong Wei, Jordan E. Pomeroy, Wange Lu, and Martin F. Pera. “Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion.Stem Cells 28, no. 8 (August 2010): 1338–48. https://doi.org/10.1002/stem.466.
Hasegawa K, Zhang P, Wei Z, Pomeroy JE, Lu W, Pera MF. Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion. Stem Cells. 2010 Aug;28(8):1338–48.
Hasegawa, Kouichi, et al. “Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion.Stem Cells, vol. 28, no. 8, Aug. 2010, pp. 1338–48. Pubmed, doi:10.1002/stem.466.
Hasegawa K, Zhang P, Wei Z, Pomeroy JE, Lu W, Pera MF. Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion. Stem Cells. 2010 Aug;28(8):1338–1348.
Journal cover image

Published In

Stem Cells

DOI

EISSN

1549-4918

Publication Date

August 2010

Volume

28

Issue

8

Start / End Page

1338 / 1348

Location

England

Related Subject Headings

  • Transduction, Genetic
  • SOXB1 Transcription Factors
  • Proto-Oncogene Proteins c-myc
  • Octamer Transcription Factor-3
  • Lentivirus
  • Kruppel-Like Transcription Factors
  • Kruppel-Like Factor 4
  • Induced Pluripotent Stem Cells
  • Immunology
  • Humans