Scholars@Duke publication: Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction.

Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction.

Publication , Journal Article

Frey, TG; Kuhn, LA; Leigh, JS; Costello, MJ; Chan, SH

Published in: J Inorg Biochem

1985

Electron microscopic images of selectively contrasted cytochrome oxidase dimer crystals are interpreted in a manner consistent with the structure of monomers determined by Fuller et al. (J. Molec. Biol. 134, 305-327). The arms of the y-shaped monomers lie within and perpendicular to the lipid bilayer protruding approximately 25 A on the matrix side of the membrane. The cytoplasmic-side tails of two monomers spread apart in a dimer forming a large cleft. Decoration of the exposed matrix side of vesicle crystals with antisubunit IV antibody fragments indicates that subunit IV lies along the a-crystal axis roughly 20 A from the center of the dimer. A membrane propensity algorithm applied to the sequences of cytochrome oxidase subunits predicts a total of 19 transmembrane alpha-helices per monomer.

Duke Scholars

Author Martin Joseph Costello III Ophthalmology

Published In

J Inorg Biochem

DOI

10.1016/0162-0134(85)85020-0

ISSN

0162-0134

Publication Date

1985

Volume

Issue

3-4

Start / End Page

155 / 162

Location

United States

Related Subject Headings

Protein Conformation
Microscopy, Electron
Membrane Proteins
Macromolecular Substances
Lipid Bilayers
Inorganic & Nuclear Chemistry
Immunologic Techniques
Immunoglobulin Fab Fragments
Freeze Fracturing
Freeze Drying

Citation

APA

Chicago

ICMJE

MLA

NLM

Frey, T. G., Kuhn, L. A., Leigh, J. S., Costello, M. J., & Chan, S. H. (1985). Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction. J Inorg Biochem, 23(3–4), 155–162. https://doi.org/10.1016/0162-0134(85)85020-0

Frey, T. G., L. A. Kuhn, J. S. Leigh, M. J. Costello, and S. H. Chan. “Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction.” J Inorg Biochem 23, no. 3–4 (1985): 155–62. https://doi.org/10.1016/0162-0134(85)85020-0.

Frey TG, Kuhn LA, Leigh JS, Costello MJ, Chan SH. Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction. J Inorg Biochem. 1985;23(3–4):155–62.

Frey, T. G., et al. “Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction.” J Inorg Biochem, vol. 23, no. 3–4, 1985, pp. 155–62. Pubmed, doi:10.1016/0162-0134(85)85020-0.

Frey TG, Kuhn LA, Leigh JS, Costello MJ, Chan SH. Cytochrome oxidase: structural insights from electron microscopy and from secondary structure prediction. J Inorg Biochem. 1985;23(3–4):155–162.