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Metabolic landscape of the tumor microenvironment at single cell resolution.

Publication ,  Journal Article
Xiao, Z; Dai, Z; Locasale, JW
Published in: Nat Commun
August 21, 2019

The tumor milieu consists of numerous cell types each existing in a different environment. However, a characterization of metabolic heterogeneity at single-cell resolution is not established. Here, we develop a computational pipeline to study metabolic programs in single cells. In two representative human cancers, melanoma and head and neck, we apply this algorithm to define the intratumor metabolic landscape. We report an overall discordance between analyses of single cells and those of bulk tumors with higher metabolic activity in malignant cells than previously appreciated. Variation in mitochondrial programs is found to be the major contributor to metabolic heterogeneity. Surprisingly, the expression of both glycolytic and mitochondrial programs strongly correlates with hypoxia in all cell types. Immune and stromal cells could also be distinguished by their metabolic features. Taken together this analysis establishes a computational framework for characterizing metabolism using single cell expression data and defines principles of the tumor microenvironment.

Duke Scholars

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

August 21, 2019

Volume

10

Issue

1

Start / End Page

3763

Location

England

Related Subject Headings

  • Tumor Microenvironment
  • Transcriptome
  • Stromal Cells
  • Neoplasms
  • Mitochondria
  • Melanoma
  • Humans
  • Glycolysis
  • Genes, Neoplasm
  • Gene Expression Regulation, Neoplastic
 

Citation

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Xiao, Z., Dai, Z., & Locasale, J. W. (2019). Metabolic landscape of the tumor microenvironment at single cell resolution. Nat Commun, 10(1), 3763. https://doi.org/10.1038/s41467-019-11738-0
Xiao, Zhengtao, Ziwei Dai, and Jason W. Locasale. “Metabolic landscape of the tumor microenvironment at single cell resolution.Nat Commun 10, no. 1 (August 21, 2019): 3763. https://doi.org/10.1038/s41467-019-11738-0.
Xiao Z, Dai Z, Locasale JW. Metabolic landscape of the tumor microenvironment at single cell resolution. Nat Commun. 2019 Aug 21;10(1):3763.
Xiao, Zhengtao, et al. “Metabolic landscape of the tumor microenvironment at single cell resolution.Nat Commun, vol. 10, no. 1, Aug. 2019, p. 3763. Pubmed, doi:10.1038/s41467-019-11738-0.
Xiao Z, Dai Z, Locasale JW. Metabolic landscape of the tumor microenvironment at single cell resolution. Nat Commun. 2019 Aug 21;10(1):3763.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

August 21, 2019

Volume

10

Issue

1

Start / End Page

3763

Location

England

Related Subject Headings

  • Tumor Microenvironment
  • Transcriptome
  • Stromal Cells
  • Neoplasms
  • Mitochondria
  • Melanoma
  • Humans
  • Glycolysis
  • Genes, Neoplasm
  • Gene Expression Regulation, Neoplastic