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Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4.

Publication ,  Journal Article
Coutavas, E; Ren, M; Oppenheim, JD; D'Eustachio, P; Rush, MG
Published in: Nature
December 9, 1993

The human Ras-related nuclear protein Ran/TC4 (refs 1-4) is the prototype of a well conserved family of GTPases that can regulate both cell-cycle progression and messenger RNA transport. Ran has been proposed to undergo tightly controlled cycles of GTP binding and hydrolysis, to operate as a GTPase switch whose GTP- and GDP-bound forms interact differentially with regulators and effectors. One known regulator, the protein RCC1 (refs 12, 13), interacts with Ran to catalyse guanine nucleotide exchange, and both RCC1 and Ran are components of an intrinsic checkpoint control that prevents the premature initiation of mitosis. To test and extend the GTPase-switch model, we searched for a Ran-specific GTPase-activating protein (GAP), and for putative effectors (proteins that interact specifically with Ran/TC4-GTP). We report here the identification of a Ran GAP and its use to characterize the GTP-hydrolysing properties of mutant Ran proteins, and the identification and cloning of a binding protein specific for Ran/TC4-GTP.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 9, 1993

Volume

366

Issue

6455

Start / End Page

585 / 587

Location

England

Related Subject Headings

  • ras GTPase-Activating Proteins
  • ran GTP-Binding Protein
  • Xenopus
  • Schizosaccharomyces
  • S100 Proteins
  • Proteins
  • Nuclear Proteins
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Humans
 

Citation

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Coutavas, E., Ren, M., Oppenheim, J. D., D’Eustachio, P., & Rush, M. G. (1993). Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. Nature, 366(6455), 585–587. https://doi.org/10.1038/366585a0
Coutavas, E., M. Ren, J. D. Oppenheim, P. D’Eustachio, and M. G. Rush. “Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4.Nature 366, no. 6455 (December 9, 1993): 585–87. https://doi.org/10.1038/366585a0.
Coutavas E, Ren M, Oppenheim JD, D’Eustachio P, Rush MG. Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. Nature. 1993 Dec 9;366(6455):585–7.
Coutavas, E., et al. “Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4.Nature, vol. 366, no. 6455, Dec. 1993, pp. 585–87. Pubmed, doi:10.1038/366585a0.
Coutavas E, Ren M, Oppenheim JD, D’Eustachio P, Rush MG. Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. Nature. 1993 Dec 9;366(6455):585–587.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 9, 1993

Volume

366

Issue

6455

Start / End Page

585 / 587

Location

England

Related Subject Headings

  • ras GTPase-Activating Proteins
  • ran GTP-Binding Protein
  • Xenopus
  • Schizosaccharomyces
  • S100 Proteins
  • Proteins
  • Nuclear Proteins
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Humans