Skip to main content

SFPQ promotes an oncogenic transcriptomic state in melanoma.

Publication ,  Journal Article
Bi, O; Anene, CA; Nsengimana, J; Shelton, M; Roberts, W; Newton-Bishop, J; Boyne, JR
Published in: Oncogene
August 2021

The multifunctional protein, splicing factor, proline- and glutamine-rich (SFPQ) has been implicated in numerous cancers often due to interaction with coding and non-coding RNAs, however, its role in melanoma remains unclear. We report that knockdown of SFPQ expression in melanoma cells decelerates several cancer-associated cell phenotypes, including cell growth, migration, epithelial to mesenchymal transition, apoptosis, and glycolysis. RIP-seq analysis revealed that the SFPQ-RNA interactome is reprogrammed in melanoma cells and specifically enriched with key melanoma-associated coding and long non-coding transcripts, including SOX10, AMIGO2 and LINC00511 and in most cases SFPQ is required for the efficient expression of these genes. Functional analysis of two SFPQ-enriched lncRNA, LINC00511 and LINC01234, demonstrated that these genes independently contribute to the melanoma phenotype and a more detailed analysis of LINC00511 indicated that this occurs in part via modulation of the miR-625-5p/PKM2 axis. Importantly, analysis of a large clinical cohort revealed that elevated expression of SFPQ in primary melanoma tumours may have utility as a prognostic biomarker. Together, these data suggest that SFPQ is an important driver of melanoma, likely due to SFPQ-RNA interactions promoting the expression of numerous oncogenic transcripts.

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Oncogene

DOI

EISSN

1476-5594

ISSN

0950-9232

Publication Date

August 2021

Volume

40

Issue

33

Start / End Page

5192 / 5203

Related Subject Headings

  • Transcriptome
  • RNA, Long Noncoding
  • Oncology & Carcinogenesis
  • Oncogenes
  • Melanoma
  • Humans
  • Epithelial-Mesenchymal Transition
  • Cell Proliferation
  • Carcinogenesis
  • 3211 Oncology and carcinogenesis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bi, O., Anene, C. A., Nsengimana, J., Shelton, M., Roberts, W., Newton-Bishop, J., & Boyne, J. R. (2021). SFPQ promotes an oncogenic transcriptomic state in melanoma. Oncogene, 40(33), 5192–5203. https://doi.org/10.1038/s41388-021-01912-4
Bi, O., C. A. Anene, J. Nsengimana, M. Shelton, W. Roberts, J. Newton-Bishop, and J. R. Boyne. “SFPQ promotes an oncogenic transcriptomic state in melanoma.Oncogene 40, no. 33 (August 2021): 5192–5203. https://doi.org/10.1038/s41388-021-01912-4.
Bi O, Anene CA, Nsengimana J, Shelton M, Roberts W, Newton-Bishop J, et al. SFPQ promotes an oncogenic transcriptomic state in melanoma. Oncogene. 2021 Aug;40(33):5192–203.
Bi, O., et al. “SFPQ promotes an oncogenic transcriptomic state in melanoma.Oncogene, vol. 40, no. 33, Aug. 2021, pp. 5192–203. Epmc, doi:10.1038/s41388-021-01912-4.
Bi O, Anene CA, Nsengimana J, Shelton M, Roberts W, Newton-Bishop J, Boyne JR. SFPQ promotes an oncogenic transcriptomic state in melanoma. Oncogene. 2021 Aug;40(33):5192–5203.

Published In

Oncogene

DOI

EISSN

1476-5594

ISSN

0950-9232

Publication Date

August 2021

Volume

40

Issue

33

Start / End Page

5192 / 5203

Related Subject Headings

  • Transcriptome
  • RNA, Long Noncoding
  • Oncology & Carcinogenesis
  • Oncogenes
  • Melanoma
  • Humans
  • Epithelial-Mesenchymal Transition
  • Cell Proliferation
  • Carcinogenesis
  • 3211 Oncology and carcinogenesis