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Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells.

Publication ,  Journal Article
Akman, SA; Forrest, GP; Doroshow, JH; Dizdaroglu, M
Published in: Mutat Res
October 1991

We examined the effect of the oxidation of plasmid pZ189 by KMnO4, which does not produce free radicals, and H2O2/Fe(2+)-diethylenetriaminepentaacetic acid (DTPA), which does, on the mutation frequency of pZ189 transfected into monkey kidney CV-1 cells. Treatment with 1.5 mM KMnO4 increased the content of certain modified bases, principally Thy and Cyt modified at C-5 and C-6, by up to 300-fold, as measured by GC/MS; however, the mutation frequency increased only 5-fold above background. 1.0 mM H2O2/0.1 mM Fe(2+)-DTPA treatment, which increased the mutation frequency 10-fold above background, increased the content of certain modified bases by up to 4-fold. Sequence analysis revealed both deletions and point mutations, with a predominance of C-G substitutions, among H2O2/Fe(2+)-DTPA-associated mutations. These data suggest that KMnO4-modified DNA is only weakly mutagenic in DNA replicating in mammalian nuclei, despite substantial production of Thy glycol and other base modifications, whereas H2O2/Fe(2+)-DTPA-modified DNA is more mutagenic. H2O2/Fe(2+)-DTPA generated mutations occur predominantly at C-G base pairs.

Duke Scholars

Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

October 1991

Volume

261

Issue

2

Start / End Page

123 / 130

Location

Netherlands

Related Subject Headings

  • Transfection
  • Sequence Homology, Nucleic Acid
  • Potassium Permanganate
  • Plasmids
  • Oxidation-Reduction
  • Oncology & Carcinogenesis
  • Mutation
  • Molecular Sequence Data
  • In Vitro Techniques
  • Hydrogen Peroxide
 

Citation

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Akman, S. A., Forrest, G. P., Doroshow, J. H., & Dizdaroglu, M. (1991). Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells. Mutat Res, 261(2), 123–130. https://doi.org/10.1016/0165-1218(91)90058-t
Akman, S. A., G. P. Forrest, J. H. Doroshow, and M. Dizdaroglu. “Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells.Mutat Res 261, no. 2 (October 1991): 123–30. https://doi.org/10.1016/0165-1218(91)90058-t.
Akman SA, Forrest GP, Doroshow JH, Dizdaroglu M. Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells. Mutat Res. 1991 Oct;261(2):123–30.
Akman, S. A., et al. “Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells.Mutat Res, vol. 261, no. 2, Oct. 1991, pp. 123–30. Pubmed, doi:10.1016/0165-1218(91)90058-t.
Akman SA, Forrest GP, Doroshow JH, Dizdaroglu M. Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells. Mutat Res. 1991 Oct;261(2):123–130.
Journal cover image

Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

October 1991

Volume

261

Issue

2

Start / End Page

123 / 130

Location

Netherlands

Related Subject Headings

  • Transfection
  • Sequence Homology, Nucleic Acid
  • Potassium Permanganate
  • Plasmids
  • Oxidation-Reduction
  • Oncology & Carcinogenesis
  • Mutation
  • Molecular Sequence Data
  • In Vitro Techniques
  • Hydrogen Peroxide