Skip to main content
Journal cover image

Mapping oxidative DNA damage and mechanisms of repair.

Publication ,  Conference
Akman, SA; O'Connor, TR; Rodriguez, H
Published in: Ann N Y Acad Sci
2000

We developed a method to map oxidative-induced DNA damage at the nucleotide level using ligation-mediated polymerase chain reaction (LMPCR) technology. In vivo and in vitro DNA base modification patterns inflicted by reactive oxygen species (ROS) in the human P53 and PGK1 gene were nearly identical in vitro and in vivo. In human male fibroblasts, these patterns are independent of the transition metal used (Cu (II), Fe(II), or Cr(VI). Therefore, local probability of H2O2-mediated DNA base damage is determined primarily by DNA sequence. Moreover, in cells undergoing severe oxidative stress, extranuclear sites contribute metals that enhance nuclear DNA damage. The role of the base excision repair pathway in human cells responsible for the repair of the majority of ROS base damage is also discussed.

Duke Scholars

Published In

Ann N Y Acad Sci

DOI

ISSN

0077-8923

Publication Date

2000

Volume

899

Start / End Page

88 / 102

Location

United States

Related Subject Headings

  • Skin
  • Reactive Oxygen Species
  • Oxidative Stress
  • Metals
  • Male
  • In Vitro Techniques
  • Hydrogen Peroxide
  • Humans
  • General Science & Technology
  • DNA Repair
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Akman, S. A., O’Connor, T. R., & Rodriguez, H. (2000). Mapping oxidative DNA damage and mechanisms of repair. In Ann N Y Acad Sci (Vol. 899, pp. 88–102). United States. https://doi.org/10.1111/j.1749-6632.2000.tb06178.x
Akman, S. A., T. R. O’Connor, and H. Rodriguez. “Mapping oxidative DNA damage and mechanisms of repair.” In Ann N Y Acad Sci, 899:88–102, 2000. https://doi.org/10.1111/j.1749-6632.2000.tb06178.x.
Akman SA, O’Connor TR, Rodriguez H. Mapping oxidative DNA damage and mechanisms of repair. In: Ann N Y Acad Sci. 2000. p. 88–102.
Akman, S. A., et al. “Mapping oxidative DNA damage and mechanisms of repair.Ann N Y Acad Sci, vol. 899, 2000, pp. 88–102. Pubmed, doi:10.1111/j.1749-6632.2000.tb06178.x.
Akman SA, O’Connor TR, Rodriguez H. Mapping oxidative DNA damage and mechanisms of repair. Ann N Y Acad Sci. 2000. p. 88–102.
Journal cover image

Published In

Ann N Y Acad Sci

DOI

ISSN

0077-8923

Publication Date

2000

Volume

899

Start / End Page

88 / 102

Location

United States

Related Subject Headings

  • Skin
  • Reactive Oxygen Species
  • Oxidative Stress
  • Metals
  • Male
  • In Vitro Techniques
  • Hydrogen Peroxide
  • Humans
  • General Science & Technology
  • DNA Repair