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Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells.

Publication ,  Journal Article
Yu, X; Feizpour, A; Ramirez, N-GP; Wu, L; Akiyama, H; Xu, F; Gummuluru, S; Reinhard, BM
Published in: Nature communications
June 2014

Ganglioside GM3, a host-derived glycosphingolipid incorporated in the membrane of human immunodeficiency virus-1 (HIV-1) viral particles, mediates interactions between HIV-1 and Siglec1/CD169, a protein expressed on dendritic cells (DCs). Such interactions, which seem to be independent of viral envelope glycoprotein gp120, are poorly understood. Here we develop a model system consisting of self-assembled artificial virus nanoparticles (AVNs) that are free of viral glycoproteins or other host-derived glycolipids and glycoproteins. These plasmonic AVNs contain a membrane of defined composition wrapped around a solid metal core. GM3-containing AVNs are captured by CD169-expressing HeLa cells or mature DCs, and are sequestered within non-lysosomal tetraspanin-positive compartments. This distribution is reminiscent of CD169-dependent HIV-1 sequestration in mature DCs. Our results highlight GM3-CD169 binding as a gp120-independent signal for sequestration and preservation of HIV-1 infectivity. They also indicate that plasmonic AVNs offer improved features over liposome-based systems and represent a versatile tool for probing specific virus-cell interactions.

Duke Scholars

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Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

June 2014

Volume

5

Start / End Page

4136

Related Subject Headings

  • Virus Internalization
  • Spectrophotometry, Ultraviolet
  • Sialic Acid Binding Ig-like Lectin 1
  • Polymerase Chain Reaction
  • Nanoparticles
  • Microscopy, Fluorescence
  • Microscopy, Electron, Transmission
  • Humans
  • Hela Cells
  • HeLa Cells
 

Citation

APA
Chicago
ICMJE
MLA
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Yu, X., Feizpour, A., Ramirez, N.-G., Wu, L., Akiyama, H., Xu, F., … Reinhard, B. M. (2014). Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells. Nature Communications, 5, 4136. https://doi.org/10.1038/ncomms5136
Yu, Xinwei, Amin Feizpour, Nora-Guadalupe P. Ramirez, Linxi Wu, Hisashi Akiyama, Fangda Xu, Suryaram Gummuluru, and Björn M. Reinhard. “Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells.Nature Communications 5 (June 2014): 4136. https://doi.org/10.1038/ncomms5136.
Yu X, Feizpour A, Ramirez N-GP, Wu L, Akiyama H, Xu F, et al. Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells. Nature communications. 2014 Jun;5:4136.
Yu, Xinwei, et al. “Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells.Nature Communications, vol. 5, June 2014, p. 4136. Epmc, doi:10.1038/ncomms5136.
Yu X, Feizpour A, Ramirez N-GP, Wu L, Akiyama H, Xu F, Gummuluru S, Reinhard BM. Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells. Nature communications. 2014 Jun;5:4136.

Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

June 2014

Volume

5

Start / End Page

4136

Related Subject Headings

  • Virus Internalization
  • Spectrophotometry, Ultraviolet
  • Sialic Acid Binding Ig-like Lectin 1
  • Polymerase Chain Reaction
  • Nanoparticles
  • Microscopy, Fluorescence
  • Microscopy, Electron, Transmission
  • Humans
  • Hela Cells
  • HeLa Cells