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Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.

Publication ,  Journal Article
Peng, Y; Yu, Y; Lin, L; Liu, X; Zhang, X; Wang, P; Hoffman, P; Kim, SY; Zhang, F; Linhardt, RJ
Published in: Glycoconj J
February 2018

Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour. In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry. The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR). The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively. The disaccharide composition of CS consisted of 4S (49%), 0S (38%) 6S (12%), 2S6S (1.5%) and 2S4S (0.3%). The disaccharide composition of HS consisted of 0S (80%), NS2S (7%), NS (7%), 6S (4%), NS6S (2%), and TriS, 2S and 4S6S (each at 0.1%). The average molecular weights of CS and HS were 148 kDa and 204 kDa, respectively. SPR reveals that collagen type II binds to heparin (primarily composed of TriS) with a binding affinity (K D) of 755 nM and interacts with other GAGs, including CSB and CSE. Both bovine vitreous CS and HS interact with collagen type II, with vitreous HS showing a higher binding affinity.

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Published In

Glycoconj J

DOI

EISSN

1573-4986

Publication Date

February 2018

Volume

35

Issue

1

Start / End Page

119 / 128

Location

United States

Related Subject Headings

  • Vitreous Body
  • Swine
  • Sharks
  • Protein Binding
  • Hyaluronic Acid
  • Heparitin Sulfate
  • Decapodiformes
  • Collagen Type II
  • Chondroitin Sulfates
  • Chickens
 

Citation

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Peng, Y., Yu, Y., Lin, L., Liu, X., Zhang, X., Wang, P., … Linhardt, R. J. (2018). Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II. Glycoconj J, 35(1), 119–128. https://doi.org/10.1007/s10719-017-9808-1
Peng, Yanfei, Yanlei Yu, Lei Lin, Xinyue Liu, Xing Zhang, Peipei Wang, Pauline Hoffman, So Young Kim, Fuming Zhang, and Robert J. Linhardt. “Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.Glycoconj J 35, no. 1 (February 2018): 119–28. https://doi.org/10.1007/s10719-017-9808-1.
Peng Y, Yu Y, Lin L, Liu X, Zhang X, Wang P, et al. Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II. Glycoconj J. 2018 Feb;35(1):119–28.
Peng, Yanfei, et al. “Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II.Glycoconj J, vol. 35, no. 1, Feb. 2018, pp. 119–28. Pubmed, doi:10.1007/s10719-017-9808-1.
Peng Y, Yu Y, Lin L, Liu X, Zhang X, Wang P, Hoffman P, Kim SY, Zhang F, Linhardt RJ. Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II. Glycoconj J. 2018 Feb;35(1):119–128.
Journal cover image

Published In

Glycoconj J

DOI

EISSN

1573-4986

Publication Date

February 2018

Volume

35

Issue

1

Start / End Page

119 / 128

Location

United States

Related Subject Headings

  • Vitreous Body
  • Swine
  • Sharks
  • Protein Binding
  • Hyaluronic Acid
  • Heparitin Sulfate
  • Decapodiformes
  • Collagen Type II
  • Chondroitin Sulfates
  • Chickens