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Orthotopic Models Using New, Murine Lung Adenocarcinoma Cell Lines Simulate Human Non-Small Cell Lung Cancer Treated with Immunotherapy.

Publication ,  Journal Article
Knott, EP; Kim, EY; Kim, EQ; Freire, R; Medina, JA; Wang, Y; Chen, C-B; Wu, C; Wangpaichitr, M; Conejo-Garcia, JR; Lim, DC
Published in: Cells
June 28, 2024

Understanding tumor-host immune interactions and the mechanisms of lung cancer response to immunotherapy is crucial. Current preclinical models used to study this often fall short of capturing the complexities of human lung cancer and lead to inconclusive results. To bridge the gap, we introduce two new murine monoclonal lung cancer cell lines for use in immunocompetent orthotopic models. We demonstrate how our cell lines exhibit immunohistochemical protein expression (TTF-1, NapA, PD-L1) and common driver mutations (KRAS, p53, and p110α) seen in human lung adenocarcinoma patients, and how our orthotopic models respond to combination immunotherapy in vivo in a way that closely mirrors current clinical outcomes. These new lung adenocarcinoma cell lines provide an invaluable, clinically relevant platform for investigating the intricate dynamics between tumor and the immune system, and thus potentially contributes to a deeper understanding of immunotherapeutic approaches to lung cancer treatment.

Duke Scholars

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Published In

Cells

DOI

EISSN

2073-4409

Publication Date

June 28, 2024

Volume

13

Issue

13

Location

Switzerland

Related Subject Headings

  • Mice
  • Lung Neoplasms
  • Immunotherapy
  • Humans
  • Female
  • Disease Models, Animal
  • Cell Line, Tumor
  • Carcinoma, Non-Small-Cell Lung
  • Animals
  • Adenocarcinoma of Lung
 

Citation

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Knott, E. P., Kim, E. Y., Kim, E. Q., Freire, R., Medina, J. A., Wang, Y., … Lim, D. C. (2024). Orthotopic Models Using New, Murine Lung Adenocarcinoma Cell Lines Simulate Human Non-Small Cell Lung Cancer Treated with Immunotherapy. Cells, 13(13). https://doi.org/10.3390/cells13131120
Knott, Eric P., Emily Y. Kim, Edison Q. Kim, Rochelle Freire, Justin A. Medina, Yujie Wang, Cheng-Bang Chen, et al. “Orthotopic Models Using New, Murine Lung Adenocarcinoma Cell Lines Simulate Human Non-Small Cell Lung Cancer Treated with Immunotherapy.Cells 13, no. 13 (June 28, 2024). https://doi.org/10.3390/cells13131120.
Knott, Eric P., et al. “Orthotopic Models Using New, Murine Lung Adenocarcinoma Cell Lines Simulate Human Non-Small Cell Lung Cancer Treated with Immunotherapy.Cells, vol. 13, no. 13, June 2024. Pubmed, doi:10.3390/cells13131120.
Knott EP, Kim EY, Kim EQ, Freire R, Medina JA, Wang Y, Chen C-B, Wu C, Wangpaichitr M, Conejo-Garcia JR, Lim DC. Orthotopic Models Using New, Murine Lung Adenocarcinoma Cell Lines Simulate Human Non-Small Cell Lung Cancer Treated with Immunotherapy. Cells. 2024 Jun 28;13(13).

Published In

Cells

DOI

EISSN

2073-4409

Publication Date

June 28, 2024

Volume

13

Issue

13

Location

Switzerland

Related Subject Headings

  • Mice
  • Lung Neoplasms
  • Immunotherapy
  • Humans
  • Female
  • Disease Models, Animal
  • Cell Line, Tumor
  • Carcinoma, Non-Small-Cell Lung
  • Animals
  • Adenocarcinoma of Lung