A simple method for testing and controlling inhibition in soil and sediment samples for qPCR.
Journal articles
- Journal Article
Bott, T; Shaw, G; Gregory, S
Published in: J Microbiol Methods
September 2023
The presence of polymerase-chain-reaction (PCR) inhibitors in many environmental samples can make reliable and repeatable quantitative-polymerase-chain-reaction (qPCR) analysis difficult without sample dilution. To estimate an optimal sample dilution for qPCR and reduce effects of inhibition, a simple test based on multiple dilution series of samples is presented that avoids the use of internal controls and standards reducing complexity and cost.
Duke Scholars
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Published In
J Microbiol Methods
DOI
EISSN
1872-8359
Publication Date
September 2023
Volume
212
Start / End Page
106795
Location
Netherlands
Related Subject Headings
- Soil
- Real-Time Polymerase Chain Reaction
- Polymerase Chain Reaction
- Microbiology
- 3107 Microbiology
Citation
APA
Chicago
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Bott, T., Shaw, G., & Gregory, S. (2023). A simple method for testing and controlling inhibition in soil and sediment samples for qPCR. J Microbiol Methods, 212, 106795. https://doi.org/10.1016/j.mimet.2023.106795
Bott, Tom, George Shaw, and Simon Gregory. “A simple method for testing and controlling inhibition in soil and sediment samples for qPCR.” J Microbiol Methods 212 (September 2023): 106795. https://doi.org/10.1016/j.mimet.2023.106795.
Bott T, Shaw G, Gregory S. A simple method for testing and controlling inhibition in soil and sediment samples for qPCR. J Microbiol Methods. 2023 Sep;212:106795.
Bott, Tom, et al. “A simple method for testing and controlling inhibition in soil and sediment samples for qPCR.” J Microbiol Methods, vol. 212, Sept. 2023, p. 106795. Pubmed, doi:10.1016/j.mimet.2023.106795.
Bott T, Shaw G, Gregory S. A simple method for testing and controlling inhibition in soil and sediment samples for qPCR. J Microbiol Methods. 2023 Sep;212:106795.
Published In
J Microbiol Methods
DOI
EISSN
1872-8359
Publication Date
September 2023
Volume
212
Start / End Page
106795
Location
Netherlands
Related Subject Headings
- Soil
- Real-Time Polymerase Chain Reaction
- Polymerase Chain Reaction
- Microbiology
- 3107 Microbiology