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Dopamine neuronal transport kinetics and effects of amphetamine.

Publication ,  Journal Article
Jones, SR; Joseph, JD; Barak, LS; Caron, MG; Wightman, RM
Published in: J Neurochem
December 1999

The dopamine (DA) transporter (DAT) regulates DA neurotransmission by recycling DA back into neurons. Drugs that interfere with DAT function, e.g., cocaine and amphetamine, can have profound behavioral effects. The kinetics of DA transport by DAT in isolated synaptosomal or single cell preparations have been previously studied. To investigate how DA transport is regulated in intact tissue and to examine how amphetamine affects the DAT, the kinetics of DA uptake by the DAT were examined in tissue slices of the mouse caudate-putamen with fast-scan cyclic voltammetry. The data demonstrate that inward DA transport is saturable and sodium-dependent. Elevated levels of cytoplasmic DA resulting from disruption of vesicular storage by incubation with 10 microM Ro 4-1284 did not generate DA efflux or decrease its uptake rate. However, incubation with 10 microM amphetamine reduced the net DA uptake rate and increased extracellular DA levels due to DA efflux through the DAT. In addition, a new, elevated steady-state level of extracellular DA was established after electrically stimulated DA release in the presence of amphetamine, norepinephrine, and exogenous DA. These results from intact tissue are consistent with a kinetic model of the DAT established in more purified preparations in which amphetamine and other transported substances make the inwardly facing DAT available for outward transport of intracellular DA.

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Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

December 1999

Volume

73

Issue

6

Start / End Page

2406 / 2414

Location

England

Related Subject Headings

  • Sodium
  • Putamen
  • Ouabain
  • Neurotransmitter Uptake Inhibitors
  • Neurons
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • Mice, Inbred C57BL
  • Mice
  • Membrane Transport Proteins
 

Citation

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Jones, S. R., Joseph, J. D., Barak, L. S., Caron, M. G., & Wightman, R. M. (1999). Dopamine neuronal transport kinetics and effects of amphetamine. J Neurochem, 73(6), 2406–2414. https://doi.org/10.1046/j.1471-4159.1999.0732406.x
Jones, S. R., J. D. Joseph, L. S. Barak, M. G. Caron, and R. M. Wightman. “Dopamine neuronal transport kinetics and effects of amphetamine.J Neurochem 73, no. 6 (December 1999): 2406–14. https://doi.org/10.1046/j.1471-4159.1999.0732406.x.
Jones SR, Joseph JD, Barak LS, Caron MG, Wightman RM. Dopamine neuronal transport kinetics and effects of amphetamine. J Neurochem. 1999 Dec;73(6):2406–14.
Jones, S. R., et al. “Dopamine neuronal transport kinetics and effects of amphetamine.J Neurochem, vol. 73, no. 6, Dec. 1999, pp. 2406–14. Pubmed, doi:10.1046/j.1471-4159.1999.0732406.x.
Jones SR, Joseph JD, Barak LS, Caron MG, Wightman RM. Dopamine neuronal transport kinetics and effects of amphetamine. J Neurochem. 1999 Dec;73(6):2406–2414.
Journal cover image

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

December 1999

Volume

73

Issue

6

Start / End Page

2406 / 2414

Location

England

Related Subject Headings

  • Sodium
  • Putamen
  • Ouabain
  • Neurotransmitter Uptake Inhibitors
  • Neurons
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • Mice, Inbred C57BL
  • Mice
  • Membrane Transport Proteins