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The role of bladder afferent pathways in bladder hyperactivity induced by the intravesical administration of nerve growth factor.

Publication ,  Journal Article
Chuang, YC; Fraser, MO; Yu, Y; Chancellor, MB; de Groat, WC; Yoshimura, N
Published in: The Journal of Urology
March 2001

Interstitial cystitis, a chronic disease of the bladder, is characterized by urinary frequency, urgency and suprapubic pain. Nerve growth factor is a substance that may sensitize afferent nerves and induce bladder hyperactivity. It is often increased in the urine of patients with interstitial cystitis. We evaluated the role of Adelta and C fiber afferents in the type of bladder hyperactivity induced by the intravesical administration of nerve growth factor.A total of 22 Wistar and 8 Sprague-Dawley adult female rats were anesthetized with 1.2 gm/kg urethane given subcutaneously. A transurethral catheter was inserted into the bladder. Some animals were pretreated with 125 mg/kg capsaicin injected subcutaneously 4 days before nerve growth factor administration. Cystometry was performed by slowly filling the bladder at a rate of 0.04 ml per minute for 15 minutes with a volume of up to 0.6 ml. Parameters measured included volume threshold and pressure threshold for inducing the micturition reflex, compliance, bladder contraction amplitude, number of contractions and the inter-contraction interval. Nerve growth factor (0.5 ml of 20 microg/ml in 10% dimethyl sulfoxide) or a vehicle solution (0.5 ml of 10% dimethyl sulfoxide) was infused into the bladder through a transurethral catheter and retained for 1 hour.In Wistar rats nerve growth factor increased the mean number of contractions by 111% versus controls (5.7 versus 2.7, p <0.05), and decreased the mean volume threshold by 41% (0.244 versus 0.412 ml, p <0.05). This effect of nerve growth factor was not detected in Sprague-Dawley rats. Capsaicin pretreatment increased the volume threshold by 59% but did not change nerve growth factor induced bladder hyperactivity.The intravesical application of nerve growth factor acutely induced bladder hyperactivity in Wistar but not in Sprague-Dawley rats. Because the C fiber afferent neurotoxin capsaicin did not change the effect of nerve growth factor, we believe that Adelta afferent neurons have a major role in nerve growth factor induced bladder hyperactivity.

Published In

The Journal of Urology

DOI

EISSN

1527-3792

ISSN

0022-5347

Publication Date

March 2001

Volume

165

Issue

3

Start / End Page

975 / 979

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Rats, Wistar
  • Rats, Sprague-Dawley
  • Rats
  • Nerve Growth Factor
  • Female
  • Capsaicin
  • Animals
  • Afferent Pathways
 

Citation

APA
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ICMJE
MLA
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Chuang, Y. C., Fraser, M. O., Yu, Y., Chancellor, M. B., de Groat, W. C., & Yoshimura, N. (2001). The role of bladder afferent pathways in bladder hyperactivity induced by the intravesical administration of nerve growth factor. The Journal of Urology, 165(3), 975–979. https://doi.org/10.1016/s0022-5347(05)66587-1
Chuang, Y. C., M. O. Fraser, Y. Yu, M. B. Chancellor, W. C. de Groat, and N. Yoshimura. “The role of bladder afferent pathways in bladder hyperactivity induced by the intravesical administration of nerve growth factor.The Journal of Urology 165, no. 3 (March 2001): 975–79. https://doi.org/10.1016/s0022-5347(05)66587-1.
Chuang YC, Fraser MO, Yu Y, Chancellor MB, de Groat WC, Yoshimura N. The role of bladder afferent pathways in bladder hyperactivity induced by the intravesical administration of nerve growth factor. The Journal of Urology. 2001 Mar;165(3):975–9.
Chuang, Y. C., et al. “The role of bladder afferent pathways in bladder hyperactivity induced by the intravesical administration of nerve growth factor.The Journal of Urology, vol. 165, no. 3, Mar. 2001, pp. 975–79. Epmc, doi:10.1016/s0022-5347(05)66587-1.
Chuang YC, Fraser MO, Yu Y, Chancellor MB, de Groat WC, Yoshimura N. The role of bladder afferent pathways in bladder hyperactivity induced by the intravesical administration of nerve growth factor. The Journal of Urology. 2001 Mar;165(3):975–979.
Journal cover image

Published In

The Journal of Urology

DOI

EISSN

1527-3792

ISSN

0022-5347

Publication Date

March 2001

Volume

165

Issue

3

Start / End Page

975 / 979

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Rats, Wistar
  • Rats, Sprague-Dawley
  • Rats
  • Nerve Growth Factor
  • Female
  • Capsaicin
  • Animals
  • Afferent Pathways