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Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization.

Publication ,  Journal Article
Espinosa-Heidmann, DG; Caicedo, A; Hernandez, EP; Csaky, KG; Cousins, SW
Published in: Invest Ophthalmol Vis Sci
November 2003

PURPOSE: The pathogenesis of choroidal neovascularization (CNV) is postulated to be driven by angiogenesis, a process in which the cellular components of the new vessel complex are derived from cells resident within an adjacent preexisting capillary. Recently, an alternative paradigm, termed postnatal vasculogenesis, has been shown to contribute to some forms of neovascularization. In vasculogenesis, the cellular components of the new vessel complex are derived from circulating vascular progenitors from bone marrow. In the current study, transplantation of green fluorescent protein (GFP)-labeled bone marrow and laser-induced CNV were combined to examine the contribution of vasculogenesis to the formation of CNV. METHODS: Ten adult C57BL/6 female mice were used as recipients for bone marrow transplantation. Bone marrow was obtained from three C57BL/6 female mice transgenic for the beta-actin promoter GFP. One month after bone marrow transplantation, CNV was induced in recipient mice by making four separate burns in the choroid of each eye with a red diode laser. Four weeks after CNV was induced, eyes of recipient mice were processed for immunohistochemistry to detect GFP and markers for vascular smooth muscle cells (alpha-smooth muscle actin, desmin, and NG2 chondroitin sulfate proteoglycan), endothelial cells (CD31, BS-1 lectin), or macrophages (F4/80). RESULTS: GFP-labeled cells represented 17% of the total cell population in the lesion. Many of the GFP-labeled cells were immunoreactive for alpha-smooth muscle actin (39%), desmin, NG2, CD31 (41%), BS-1 lectin, or F4/80. GFP-labeled cells were morphologically indistinguishable from cells normally present in CNV lesions. CONCLUSIONS: This study is the first to demonstrate that bone marrow-derived progenitor cells are a source of endothelial and smooth musclelike cells in CNV.

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Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

November 2003

Volume

44

Issue

11

Start / End Page

4914 / 4919

Location

United States

Related Subject Headings

  • Proteoglycans
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Ophthalmology & Optometry
  • Muscle, Smooth, Vascular
  • Mice, Inbred C57BL
  • Mice
  • Luminescent Proteins
  • Laser Coagulation
  • Indicators and Reagents
  • Hematopoietic Stem Cells
 

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Espinosa-Heidmann, D. G., Caicedo, A., Hernandez, E. P., Csaky, K. G., & Cousins, S. W. (2003). Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization. Invest Ophthalmol Vis Sci, 44(11), 4914–4919. https://doi.org/10.1167/iovs.03-0371
Espinosa-Heidmann, Diego G., Alejandro Caicedo, Eleut P. Hernandez, Karl G. Csaky, and Scott W. Cousins. “Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization.Invest Ophthalmol Vis Sci 44, no. 11 (November 2003): 4914–19. https://doi.org/10.1167/iovs.03-0371.
Espinosa-Heidmann DG, Caicedo A, Hernandez EP, Csaky KG, Cousins SW. Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Nov;44(11):4914–9.
Espinosa-Heidmann, Diego G., et al. “Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization.Invest Ophthalmol Vis Sci, vol. 44, no. 11, Nov. 2003, pp. 4914–19. Pubmed, doi:10.1167/iovs.03-0371.
Espinosa-Heidmann DG, Caicedo A, Hernandez EP, Csaky KG, Cousins SW. Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Nov;44(11):4914–4919.

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

November 2003

Volume

44

Issue

11

Start / End Page

4914 / 4919

Location

United States

Related Subject Headings

  • Proteoglycans
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Ophthalmology & Optometry
  • Muscle, Smooth, Vascular
  • Mice, Inbred C57BL
  • Mice
  • Luminescent Proteins
  • Laser Coagulation
  • Indicators and Reagents
  • Hematopoietic Stem Cells