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Substance P and substance K receptor binding sites in the human gastrointestinal tract: localization by autoradiography.

Publication ,  Journal Article
Gates, TS; Zimmerman, RP; Mantyh, CR; Vigna, SR; Maggio, JE; Welton, ML; Passaro, EP; Mantyh, PW
Published in: Peptides
1988

Quantitative receptor autoradiography was used to localize and quantify the distribution of binding sites for 125I-radiolabeled substance P (SP), substance K (SK) and neuromedin K (NK) in the human GI tract using histologically normal tissue obtained from uninvolved margins of resections for carcinoma. The distribution of SP and SK binding sites is different for each gastrointestinal (GI) segment examined. Specific SP binding sites are expressed by arterioles and venules, myenteric plexus, external circular muscle, external longitudinal muscle, muscularis mucosa, epithelial cells of the mucosa, and the germinal centers of lymph nodules. SK binding sites are distributed in a pattern distinct from SP binding sites and are localized to the external circular muscle, external longitudinal muscle, and the muscularis mucosa. Binding sites for NK were not detected in any part of the human GI tract. These results demonstrate that: 1) surgical specimens from the human GI tract can be effectively processed for quantitative receptor autoradiography; 2) of the three mammalian tachykinins tested, SP and SK, but not NK binding sites are expressed in detectable levels in the human GI tract; 3) whereas SK receptor binding sites are expressed almost exclusively by smooth muscle, SP binding sites are expressed by smooth muscle cells, arterioles, venules, epithelial cells of the mucosa and cells associated with lymph nodules; and 4) both SP and SK binding sites expressed by smooth muscle are more stable than SP binding sites expressed by blood vessels, lymph nodules, and mucosal cells.

Duke Scholars

Published In

Peptides

DOI

ISSN

0196-9781

Publication Date

1988

Volume

9

Issue

6

Start / End Page

1207 / 1219

Location

United States

Related Subject Headings

  • Substance P
  • Receptors, Neurotransmitter
  • Receptors, Neurokinin-2
  • Receptors, Neurokinin-1
  • Neurokinin A
  • Molecular Sequence Data
  • Iodine Radioisotopes
  • Humans
  • Digestive System
  • Autoradiography
 

Citation

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Chicago
ICMJE
MLA
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Gates, T. S., Zimmerman, R. P., Mantyh, C. R., Vigna, S. R., Maggio, J. E., Welton, M. L., … Mantyh, P. W. (1988). Substance P and substance K receptor binding sites in the human gastrointestinal tract: localization by autoradiography. Peptides, 9(6), 1207–1219. https://doi.org/10.1016/0196-9781(88)90184-2
Gates, T. S., R. P. Zimmerman, C. R. Mantyh, S. R. Vigna, J. E. Maggio, M. L. Welton, E. P. Passaro, and P. W. Mantyh. “Substance P and substance K receptor binding sites in the human gastrointestinal tract: localization by autoradiography.Peptides 9, no. 6 (1988): 1207–19. https://doi.org/10.1016/0196-9781(88)90184-2.
Gates TS, Zimmerman RP, Mantyh CR, Vigna SR, Maggio JE, Welton ML, et al. Substance P and substance K receptor binding sites in the human gastrointestinal tract: localization by autoradiography. Peptides. 1988;9(6):1207–19.
Gates, T. S., et al. “Substance P and substance K receptor binding sites in the human gastrointestinal tract: localization by autoradiography.Peptides, vol. 9, no. 6, 1988, pp. 1207–19. Pubmed, doi:10.1016/0196-9781(88)90184-2.
Gates TS, Zimmerman RP, Mantyh CR, Vigna SR, Maggio JE, Welton ML, Passaro EP, Mantyh PW. Substance P and substance K receptor binding sites in the human gastrointestinal tract: localization by autoradiography. Peptides. 1988;9(6):1207–1219.
Journal cover image

Published In

Peptides

DOI

ISSN

0196-9781

Publication Date

1988

Volume

9

Issue

6

Start / End Page

1207 / 1219

Location

United States

Related Subject Headings

  • Substance P
  • Receptors, Neurotransmitter
  • Receptors, Neurokinin-2
  • Receptors, Neurokinin-1
  • Neurokinin A
  • Molecular Sequence Data
  • Iodine Radioisotopes
  • Humans
  • Digestive System
  • Autoradiography