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A model system for the biochemical study of luteinizing hormone/chorionic gonadotropin receptor synthesis.

Publication ,  Journal Article
Segaloff, DL; May, J; Schomberg, DW; Limbird, LE
Published in: Biochim Biophys Acta
May 22, 1984

A model system for the biochemical study of LH/CG receptor synthesis has been developed. Culture conditions for porcine granulosa cells were adapted that maximized the selective induction of LH/CG receptors by cAMP-inducing stimuli with an elimination of background LH/CG receptor appearance. It was found that the addition of FSH (1.5 micrograms/ml) or cholera toxin (10 ng/ml) 1 day after plating resulted in optimal induction of the LH/CG receptor (20-60 pg [125I]CG bound/micrograms DNA 72 h after addition) with virtually no LH/CG receptor appearance in the absence of added stimuli. Later additions of FSH or cholera toxin required insulin (1.0 microgram/ml) which alone caused background LH/CG receptor appearance in the absence of any additional stimuli. Furthermore, insulin increased the general rate of cellular protein synthesis, whereas FSH or cholera toxin each decreased it. Thus, the use of FSH or cholera toxin, without insulin, may enable one to detect the synthesis of the LH/CG receptor by metabolic labeling techniques where background protein synthesis has been lowered.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

May 22, 1984

Volume

804

Issue

1

Start / End Page

31 / 36

Location

Netherlands

Related Subject Headings

  • Time Factors
  • Swine
  • Receptors, LH
  • Receptors, Cell Surface
  • Insulin
  • Granulosa Cells
  • Follicle Stimulating Hormone
  • Female
  • Cholera Toxin
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
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Segaloff, D. L., May, J., Schomberg, D. W., & Limbird, L. E. (1984). A model system for the biochemical study of luteinizing hormone/chorionic gonadotropin receptor synthesis. Biochim Biophys Acta, 804(1), 31–36. https://doi.org/10.1016/0167-4889(84)90095-8
Segaloff, D. L., J. May, D. W. Schomberg, and L. E. Limbird. “A model system for the biochemical study of luteinizing hormone/chorionic gonadotropin receptor synthesis.Biochim Biophys Acta 804, no. 1 (May 22, 1984): 31–36. https://doi.org/10.1016/0167-4889(84)90095-8.
Segaloff DL, May J, Schomberg DW, Limbird LE. A model system for the biochemical study of luteinizing hormone/chorionic gonadotropin receptor synthesis. Biochim Biophys Acta. 1984 May 22;804(1):31–6.
Segaloff, D. L., et al. “A model system for the biochemical study of luteinizing hormone/chorionic gonadotropin receptor synthesis.Biochim Biophys Acta, vol. 804, no. 1, May 1984, pp. 31–36. Pubmed, doi:10.1016/0167-4889(84)90095-8.
Segaloff DL, May J, Schomberg DW, Limbird LE. A model system for the biochemical study of luteinizing hormone/chorionic gonadotropin receptor synthesis. Biochim Biophys Acta. 1984 May 22;804(1):31–36.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

May 22, 1984

Volume

804

Issue

1

Start / End Page

31 / 36

Location

Netherlands

Related Subject Headings

  • Time Factors
  • Swine
  • Receptors, LH
  • Receptors, Cell Surface
  • Insulin
  • Granulosa Cells
  • Follicle Stimulating Hormone
  • Female
  • Cholera Toxin
  • Cells, Cultured