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Differential gene expression in mouse sclera during ocular development.

Publication ,  Journal Article
Zhou, J; Rappaport, EF; Tobias, JW; Young, TL
Published in: Invest Ophthalmol Vis Sci
May 2006

PURPOSE: Ocular development involves changes in extracellular matrix components of the scleral wall as it expands. This study was conducted to determine scleral gene expression profiles during mouse ocular development to identify genes involved in normal scleral growth. METHODS: Sample sets of pooled sclerae of 3- and 8-week-old mice were microdissected, and total RNA was isolated. After reverse transcription, the cDNA was in vitro transcribed to produce biotin-labeled cRNA. The purified biotin-labeled cRNA samples were hybridized to microarray chips (GeneChip Mouse Genome 430 2.0; Affymetrix, Santa Clara, CA). Gene transcript expression profiles were determined, and eight differentially expressed genes between the two age groups underwent further confirmation by real-time PCR analysis. RESULTS: Differential regulation of 4884 gene transcripts in mouse sclera with less than 5% false-discovery rate (FDR) was identified. The top 1000 with the lowest FDR among the 4884 probe sets were filtered for threefold changes between the two age groups, and 718 gene transcripts were identified. Among these 718 gene transcripts, 210 were upregulated and 508 downregulated in adult relative to juvenile mouse sclera. TGF-beta1 and several collagen genes were significantly downregulated. Microarray differential expression by real-time PCR validation of eight extracellular matrix-associated gene transcripts was confirmed. CONCLUSIONS: This is the first study to demonstrate gene expression profiles in mouse sclera during ocular growth. These findings support the role of TGFbeta1 as a signaling molecule in modulating extracellular matrix during ocular development. This endeavor may be helpful in furthering understanding of how scleral remodeling is regulated during eye growth.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

May 2006

Volume

47

Issue

5

Start / End Page

1794 / 1802

Location

United States

Related Subject Headings

  • Sclera
  • Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Oligonucleotide Array Sequence Analysis
  • Mice, Inbred C57BL
  • Mice
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Eye Proteins
  • Eye
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhou, J., Rappaport, E. F., Tobias, J. W., & Young, T. L. (2006). Differential gene expression in mouse sclera during ocular development. Invest Ophthalmol Vis Sci, 47(5), 1794–1802. https://doi.org/10.1167/iovs.05-0759
Zhou, Jie, Eric F. Rappaport, John W. Tobias, and Terri L. Young. “Differential gene expression in mouse sclera during ocular development.Invest Ophthalmol Vis Sci 47, no. 5 (May 2006): 1794–1802. https://doi.org/10.1167/iovs.05-0759.
Zhou J, Rappaport EF, Tobias JW, Young TL. Differential gene expression in mouse sclera during ocular development. Invest Ophthalmol Vis Sci. 2006 May;47(5):1794–802.
Zhou, Jie, et al. “Differential gene expression in mouse sclera during ocular development.Invest Ophthalmol Vis Sci, vol. 47, no. 5, May 2006, pp. 1794–802. Pubmed, doi:10.1167/iovs.05-0759.
Zhou J, Rappaport EF, Tobias JW, Young TL. Differential gene expression in mouse sclera during ocular development. Invest Ophthalmol Vis Sci. 2006 May;47(5):1794–1802.

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

May 2006

Volume

47

Issue

5

Start / End Page

1794 / 1802

Location

United States

Related Subject Headings

  • Sclera
  • Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Oligonucleotide Array Sequence Analysis
  • Mice, Inbred C57BL
  • Mice
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Eye Proteins
  • Eye