Scholars@Duke publication: High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines.

High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines.

Publication , Journal Article

Zhu, H; Lam, DCL; Han, KC; Tin, VPC; Suen, WS; Wang, E; Lam, WK; Cai, WW; Chung, LP; Wong, MP

Published in: Cancer Lett

January 8, 2007

Tumours develop from clonally expanded population of cells harbouring aberrations of oncogenes and tumour suppressor genes. In this study, metaphase and array comparative genomic hybridization showed good correlation of aberration profiles in lung adenocarcinoma cell lines from patients with different tobacco exposure. Recurrent DNA gains were found at chromosomes 1, 7, 8, 17, 20, and deletions at 1, 3, 8, 9, 10, 12, 17, 18, 19. Candidate tumour loci and encompassed genes at 7p21 (AGR2), 8q21(TPD52), 20q13 (ZNF217, WFDC2, EEF1A2) and 10p15 (KLF6) were analyzed by dual colour FISH for genomic changes and quantitative PCR for expression changes. Results indicated that EEF1A2 and KLF6 were strong candidates of oncogene and tumour suppressor genes, respectively. This study illustrates, a practical strategy for identifying candidate cancer genes from microarray data.

Published In

Cancer Lett

DOI

10.1016/j.canlet.2006.01.020

ISSN

0304-3835

Publication Date

January 8, 2007

Volume

245

Issue

1-2

Start / End Page

303 / 314

Location

Ireland

Related Subject Headings

Tumor Cells, Cultured
Reverse Transcriptase Polymerase Chain Reaction
Oncology & Carcinogenesis
Nucleic Acid Hybridization
Middle Aged
Microarray Analysis
Metaphase
Male
Lung Neoplasms
In Situ Hybridization, Fluorescence

Citation

APA

Chicago

ICMJE

MLA

NLM

Zhu, H., Lam, D. C. L., Han, K. C., Tin, V. P. C., Suen, W. S., Wang, E., … Wong, M. P. (2007). High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines. Cancer Lett, 245(1–2), 303–314. https://doi.org/10.1016/j.canlet.2006.01.020

Zhu, Hong, David Chi Leung Lam, Kam Chu Han, Vicky Pui Chi Tin, Wai Sing Suen, Elaine Wang, Wah Kit Lam, Wei Wen Cai, Lap Ping Chung, and Maria Pik Wong. “High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines.” Cancer Lett 245, no. 1–2 (January 8, 2007): 303–14. https://doi.org/10.1016/j.canlet.2006.01.020.

Zhu H, Lam DCL, Han KC, Tin VPC, Suen WS, Wang E, et al. High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines. Cancer Lett. 2007 Jan 8;245(1–2):303–14.

Zhu, Hong, et al. “High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines.” Cancer Lett, vol. 245, no. 1–2, Jan. 2007, pp. 303–14. Pubmed, doi:10.1016/j.canlet.2006.01.020.

Zhu H, Lam DCL, Han KC, Tin VPC, Suen WS, Wang E, Lam WK, Cai WW, Chung LP, Wong MP. High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines. Cancer Lett. 2007 Jan 8;245(1–2):303–314.