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Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells.

Publication ,  Journal Article
Zhang, F; Zhang, P; Wang, E; Su, F; Qi, Y; Bu, X; Wang, Y
Published in: Biotech Histochem
July 1995

We investigated the accuracy of fluorescein diacetate uptake as an indicator of the viability of human fetal cerebral cortical cells. Cortical cells from 16-26-week-old normal fetuses were studied. The cortices were dissociated mechanically with normal saline to make a suspension. Fluorescein diacetate uptake and trypan blue exclusion were compared as methods for examining cell viability. Our results show that fluorescein diacetate uptake is a simple and sensitive method for examining human fetal cortical cell viability.

Published In

Biotech Histochem

DOI

ISSN

1052-0295

Publication Date

July 1995

Volume

70

Issue

4

Start / End Page

185 / 187

Location

England

Related Subject Headings

  • Trypan Blue
  • Sensitivity and Specificity
  • Pregnancy
  • Pathology
  • Humans
  • Fluoresceins
  • Fetal Tissue Transplantation
  • Female
  • Cerebral Cortex
  • Cell Survival
 

Citation

APA
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ICMJE
MLA
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Zhang, F., Zhang, P., Wang, E., Su, F., Qi, Y., Bu, X., & Wang, Y. (1995). Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells. Biotech Histochem, 70(4), 185–187. https://doi.org/10.3109/10520299509107310
Zhang, F., P. Zhang, E. Wang, F. Su, Y. Qi, X. Bu, and Y. Wang. “Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells.Biotech Histochem 70, no. 4 (July 1995): 185–87. https://doi.org/10.3109/10520299509107310.
Zhang F, Zhang P, Wang E, Su F, Qi Y, Bu X, et al. Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells. Biotech Histochem. 1995 Jul;70(4):185–7.
Zhang, F., et al. “Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells.Biotech Histochem, vol. 70, no. 4, July 1995, pp. 185–87. Pubmed, doi:10.3109/10520299509107310.
Zhang F, Zhang P, Wang E, Su F, Qi Y, Bu X, Wang Y. Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells. Biotech Histochem. 1995 Jul;70(4):185–187.
Journal cover image

Published In

Biotech Histochem

DOI

ISSN

1052-0295

Publication Date

July 1995

Volume

70

Issue

4

Start / End Page

185 / 187

Location

England

Related Subject Headings

  • Trypan Blue
  • Sensitivity and Specificity
  • Pregnancy
  • Pathology
  • Humans
  • Fluoresceins
  • Fetal Tissue Transplantation
  • Female
  • Cerebral Cortex
  • Cell Survival