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Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA.

Publication ,  Journal Article
Daefler, S; Klotman, ME; Wong-Staal, F
Published in: Proc Natl Acad Sci U S A
June 1990

The 20-kDa phosphorylated rev protein from human immunodeficiency virus 1 has been shown to transactivate posttranscriptionally the expression of viral structural proteins by selective stabilization and nuclear export of unspliced and incompletely spliced viral mRNA. We could demonstrate in gel-mobility and immunoprecipitation assays that the recombinant rev protein purified from a baculovirus expression system forms a distinct and specific complex with its target RNA (rev-responsive element), a 234-nucleotide sequence within the envelope coding region of human immunodeficiency virus 1. No complex formation could be observed using RNAs with similar secondary structure nor with other human immunodeficiency virus 1 recombinant proteins. Deletion analysis mapped this specific binding to the first 90 nucleotides of this rev-responsive element, which contains a U2 small nuclear RNA homologous region. We propose that the specific binding of rev to its target RNA sequence plays an essential part in releasing an incompletely spliced viral mRNA containing this target sequence to the cytoplasm.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1990

Volume

87

Issue

12

Start / End Page

4571 / 4575

Location

United States

Related Subject Headings

  • rev Gene Products, Human Immunodeficiency Virus
  • Viral Structural Proteins
  • Transfection
  • Transcriptional Activation
  • Trans-Activators
  • Sequence Homology, Nucleic Acid
  • Recombinant Proteins
  • RNA, Viral
  • RNA, Small Nuclear
  • Protein Binding
 

Citation

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Daefler, S., Klotman, M. E., & Wong-Staal, F. (1990). Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA. Proc Natl Acad Sci U S A, 87(12), 4571–4575. https://doi.org/10.1073/pnas.87.12.4571
Daefler, S., M. E. Klotman, and F. Wong-Staal. “Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA.Proc Natl Acad Sci U S A 87, no. 12 (June 1990): 4571–75. https://doi.org/10.1073/pnas.87.12.4571.
Daefler S, Klotman ME, Wong-Staal F. Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4571–5.
Daefler, S., et al. “Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA.Proc Natl Acad Sci U S A, vol. 87, no. 12, June 1990, pp. 4571–75. Pubmed, doi:10.1073/pnas.87.12.4571.
Daefler S, Klotman ME, Wong-Staal F. Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4571–4575.

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1990

Volume

87

Issue

12

Start / End Page

4571 / 4575

Location

United States

Related Subject Headings

  • rev Gene Products, Human Immunodeficiency Virus
  • Viral Structural Proteins
  • Transfection
  • Transcriptional Activation
  • Trans-Activators
  • Sequence Homology, Nucleic Acid
  • Recombinant Proteins
  • RNA, Viral
  • RNA, Small Nuclear
  • Protein Binding