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Proteomics characterization of cell membrane blebs in human retinal pigment epithelium cells.

Publication ,  Journal Article
Alcazar, O; Hawkridge, AM; Collier, TS; Cousins, SW; Bhattacharya, SK; Muddiman, DC; Marin-Castano, ME
Published in: Mol Cell Proteomics
October 2009

Age-related macular degeneration (AMD) is the leading cause of legal blindness among the elderly population in the industrialized world, affecting about 14 million people in the United States alone. Smoking is a major environmental risk factor for AMD, and hydroquinone is a major component in cigarette smoke. Hydroquinone induces the formation of cell membrane blebs in human retinal pigment epithelium (RPE). Blebs may accumulate and eventually contribute first to sub-RPE deposits and then drusen formation, which is a prominent histopathologic feature in eyes with AMD. As an attempt to better understand the mechanisms involved in early AMD, we sought to investigate the proteomic profile of RPE blebs. Isolated blebs were subjected to SDS-PAGE fractionation, and in-gel trypsin-digested peptides were analyzed by LC-MS/MS that lead to the identification of a total of 314 proteins. Identified proteins were predominantly involved in oxidative phosphorylation, cell junction, focal adhesion, cytoskeleton regulation, and immunogenic processes. Importantly basigin and matrix metalloproteinase-14, key proteins involved in extracellular matrix remodeling, were identified in RPE blebs and shown to be more prevalent in AMD patients. Altogether our findings suggest, for the first time, the potential involvement of RPE blebs in eye disease and shed light on the implication of cell-derived microvesicles in human pathology.

Duke Scholars

Published In

Mol Cell Proteomics

DOI

EISSN

1535-9484

Publication Date

October 2009

Volume

8

Issue

10

Start / End Page

2201 / 2211

Location

United States

Related Subject Headings

  • Tandem Mass Spectrometry
  • Smoking
  • Smoke
  • Retinal Pigment Epithelium
  • Recombinant Fusion Proteins
  • Proteomics
  • Nicotiana
  • Molecular Sequence Data
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 14
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Alcazar, O., Hawkridge, A. M., Collier, T. S., Cousins, S. W., Bhattacharya, S. K., Muddiman, D. C., & Marin-Castano, M. E. (2009). Proteomics characterization of cell membrane blebs in human retinal pigment epithelium cells. Mol Cell Proteomics, 8(10), 2201–2211. https://doi.org/10.1074/mcp.M900203-MCP200
Alcazar, Oscar, Adam M. Hawkridge, Timothy S. Collier, Scott W. Cousins, Sanjoy K. Bhattacharya, David C. Muddiman, and Maria E. Marin-Castano. “Proteomics characterization of cell membrane blebs in human retinal pigment epithelium cells.Mol Cell Proteomics 8, no. 10 (October 2009): 2201–11. https://doi.org/10.1074/mcp.M900203-MCP200.
Alcazar O, Hawkridge AM, Collier TS, Cousins SW, Bhattacharya SK, Muddiman DC, et al. Proteomics characterization of cell membrane blebs in human retinal pigment epithelium cells. Mol Cell Proteomics. 2009 Oct;8(10):2201–11.
Alcazar, Oscar, et al. “Proteomics characterization of cell membrane blebs in human retinal pigment epithelium cells.Mol Cell Proteomics, vol. 8, no. 10, Oct. 2009, pp. 2201–11. Pubmed, doi:10.1074/mcp.M900203-MCP200.
Alcazar O, Hawkridge AM, Collier TS, Cousins SW, Bhattacharya SK, Muddiman DC, Marin-Castano ME. Proteomics characterization of cell membrane blebs in human retinal pigment epithelium cells. Mol Cell Proteomics. 2009 Oct;8(10):2201–2211.

Published In

Mol Cell Proteomics

DOI

EISSN

1535-9484

Publication Date

October 2009

Volume

8

Issue

10

Start / End Page

2201 / 2211

Location

United States

Related Subject Headings

  • Tandem Mass Spectrometry
  • Smoking
  • Smoke
  • Retinal Pigment Epithelium
  • Recombinant Fusion Proteins
  • Proteomics
  • Nicotiana
  • Molecular Sequence Data
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 14