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Erythropoietin receptor signals both proliferation and erythroid-specific differentiation.

Publication ,  Journal Article
Liboi, E; Carroll, M; D'Andrea, AD; Mathey-Prevot, B
Published in: Proc Natl Acad Sci U S A
December 1, 1993

Ectopic expression of the erythropoietin receptor (EPO-R) in Ba/F3, an interleukin 3-dependent progenitor cell line, confers EPO-dependent cell growth. To examine whether the introduced EPO-R could affect differentiation, we isolated Ba/F3-EPO-R subclones in interleukin 3 and assayed for the induction of beta-globin mRNA synthesis after exposure to EPO. Detection of beta-globin mRNA was observed within 3 days of EPO treatment, with peak levels accumulating after 10 days. When EPO was withdrawn, expression of beta-globin mRNA persisted in most clones, suggesting that commitment to erythroid differentiation had occurred. Although EPO-R expression also supports EPO-dependent proliferation of CTLL-2, a mature T-cell line, those cells did not produce globin transcripts, presumably because they lack requisite cellular factors involved in erythrocyte differentiation. We conclude that the EPO-R transmits signals important for both proliferation and differentiation along the erythroid lineage.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1, 1993

Volume

90

Issue

23

Start / End Page

11351 / 11355

Location

United States

Related Subject Headings

  • Transfection
  • Receptors, Interleukin-3
  • Receptors, Erythropoietin
  • RNA, Messenger
  • Mice
  • Globins
  • Gene Expression Regulation
  • Erythropoietin
  • Erythropoiesis
  • Cell Line
 

Citation

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Liboi, E., Carroll, M., D’Andrea, A. D., & Mathey-Prevot, B. (1993). Erythropoietin receptor signals both proliferation and erythroid-specific differentiation. Proc Natl Acad Sci U S A, 90(23), 11351–11355. https://doi.org/10.1073/pnas.90.23.11351
Liboi, E., M. Carroll, A. D. D’Andrea, and B. Mathey-Prevot. “Erythropoietin receptor signals both proliferation and erythroid-specific differentiation.Proc Natl Acad Sci U S A 90, no. 23 (December 1, 1993): 11351–55. https://doi.org/10.1073/pnas.90.23.11351.
Liboi E, Carroll M, D’Andrea AD, Mathey-Prevot B. Erythropoietin receptor signals both proliferation and erythroid-specific differentiation. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11351–5.
Liboi, E., et al. “Erythropoietin receptor signals both proliferation and erythroid-specific differentiation.Proc Natl Acad Sci U S A, vol. 90, no. 23, Dec. 1993, pp. 11351–55. Pubmed, doi:10.1073/pnas.90.23.11351.
Liboi E, Carroll M, D’Andrea AD, Mathey-Prevot B. Erythropoietin receptor signals both proliferation and erythroid-specific differentiation. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11351–11355.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1, 1993

Volume

90

Issue

23

Start / End Page

11351 / 11355

Location

United States

Related Subject Headings

  • Transfection
  • Receptors, Interleukin-3
  • Receptors, Erythropoietin
  • RNA, Messenger
  • Mice
  • Globins
  • Gene Expression Regulation
  • Erythropoietin
  • Erythropoiesis
  • Cell Line