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Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines.

Publication ,  Journal Article
Niemeyer, CM; Sieff, CA; Mathey-Prevot, B; Wimperis, JZ; Bierer, BE; Clark, SC; Nathan, DG
Published in: Blood
March 1989

While the cellular sources for granulocyte-macrophage colony-stimulating factor (GM-CSF) are known to be widely distributed among several cell types, interleukin-3 (IL-3) gene expression has been demonstrated in only certain T-cell clones and in blood mononuclear cells stimulated with phytohemagglutinin (PHA) and phorbol-myristate-acetate (PMA). To determine which blood cells were responsible for this expression, we fractionated PHA/PMA-stimulated mononuclear cells and identified T lymphocytes as the source of IL-3 mRNA. Low-level IL-3 expression was detected as well in several stimulated human T-cell lines. Hematopoietic stromal cells such as fibroblasts and endothelial cells could not be induced to express IL-3 mRNA. The kinetics of IL-3 mRNA induction in mononuclear cells and lymphocytes stimulated with PHA/PMA or anti-CD3 monoclonal antibody (MoAb) and interleukin-1 (IL-1) were similar to those observed for GM-CSF expression.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

March 1989

Volume

73

Issue

4

Start / End Page

945 / 951

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • RNA, Messenger
  • Lymphocytes
  • Interleukin-3
  • Immunology
  • Humans
  • Growth Substances
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Gene Expression Regulation
 

Citation

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Chicago
ICMJE
MLA
NLM
Niemeyer, C. M., Sieff, C. A., Mathey-Prevot, B., Wimperis, J. Z., Bierer, B. E., Clark, S. C., & Nathan, D. G. (1989). Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines. Blood, 73(4), 945–951.
Niemeyer, C. M., C. A. Sieff, B. Mathey-Prevot, J. Z. Wimperis, B. E. Bierer, S. C. Clark, and D. G. Nathan. “Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines.Blood 73, no. 4 (March 1989): 945–51.
Niemeyer CM, Sieff CA, Mathey-Prevot B, Wimperis JZ, Bierer BE, Clark SC, et al. Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines. Blood. 1989 Mar;73(4):945–51.
Niemeyer, C. M., et al. “Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines.Blood, vol. 73, no. 4, Mar. 1989, pp. 945–51.
Niemeyer CM, Sieff CA, Mathey-Prevot B, Wimperis JZ, Bierer BE, Clark SC, Nathan DG. Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines. Blood. 1989 Mar;73(4):945–951.

Published In

Blood

ISSN

0006-4971

Publication Date

March 1989

Volume

73

Issue

4

Start / End Page

945 / 951

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • RNA, Messenger
  • Lymphocytes
  • Interleukin-3
  • Immunology
  • Humans
  • Growth Substances
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Gene Expression Regulation