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Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.

Publication ,  Journal Article
Moseley, MA; Deterding, LJ; Tomer, KB; Jorgenson, JW
Published in: Anal Chem
January 15, 1991

Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410,000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.

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Published In

Anal Chem

DOI

ISSN

0003-2700

Publication Date

January 15, 1991

Volume

63

Issue

2

Start / End Page

109 / 114

Location

United States

Related Subject Headings

  • Spectrometry, Mass, Fast Atom Bombardment
  • Oligopeptides
  • Neuropeptides
  • Molecular Sequence Data
  • Electrophoresis
  • Chemotactic Factors
  • Analytical Chemistry
  • Amino Acid Sequence
  • Absorption
  • 4004 Chemical engineering
 

Citation

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Moseley, M. A., Deterding, L. J., Tomer, K. B., & Jorgenson, J. W. (1991). Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry. Anal Chem, 63(2), 109–114. https://doi.org/10.1021/ac00002a005
Moseley, M. A., L. J. Deterding, K. B. Tomer, and J. W. Jorgenson. “Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.Anal Chem 63, no. 2 (January 15, 1991): 109–14. https://doi.org/10.1021/ac00002a005.
Moseley MA, Deterding LJ, Tomer KB, Jorgenson JW. Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry. Anal Chem. 1991 Jan 15;63(2):109–14.
Moseley, M. A., et al. “Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.Anal Chem, vol. 63, no. 2, Jan. 1991, pp. 109–14. Pubmed, doi:10.1021/ac00002a005.
Moseley MA, Deterding LJ, Tomer KB, Jorgenson JW. Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry. Anal Chem. 1991 Jan 15;63(2):109–114.
Journal cover image

Published In

Anal Chem

DOI

ISSN

0003-2700

Publication Date

January 15, 1991

Volume

63

Issue

2

Start / End Page

109 / 114

Location

United States

Related Subject Headings

  • Spectrometry, Mass, Fast Atom Bombardment
  • Oligopeptides
  • Neuropeptides
  • Molecular Sequence Data
  • Electrophoresis
  • Chemotactic Factors
  • Analytical Chemistry
  • Amino Acid Sequence
  • Absorption
  • 4004 Chemical engineering