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Differential localization of placental extracellular superoxide dismutase as pregnancy progresses.

Publication ,  Journal Article
Boggess, KA; Kay, HH; Crapo, JD; Moore, WF; Suliman, HB; Oury, TD
Published in: Am J Obstet Gynecol
July 2000

OBJECTIVE: The aim of this study was to determine placental localization and activity of extracellular superoxide dismutase, a nitric oxide modulator, during early gestation and to correlate these characteristics with fetal vascular development. STUDY DESIGN: First-trimester (n = 10) and second-trimester (n = 10) villi were obtained at elective pregnancy termination. Extracellular superoxide dismutase was localized by means of an immunoperoxidase method. Activity was measured by determining the inhibition of cytochrome c reduction at pH 10 and messenger ribonucleic acid expression by in situ hybridization. RESULTS: Extracellular superoxide dismutase was intracellular within villous trophoblasts until 17 weeks' gestation, when it relocated to the villous extracellular matrix. Activities were similar between first- and second-trimester villi. In situ hybridization confirmed extracellular superoxide dismutase messenger ribonucleic acid within trophoblasts throughout gestation. CONCLUSION: Extracellular superoxide dismutase is produced by trophoblasts early in pregnancy, but it remains intracellular until 17 weeks' gestation, which may be related to fetal vascular development.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

July 2000

Volume

183

Issue

1

Start / End Page

199 / 205

Location

United States

Related Subject Headings

  • Trophoblasts
  • Superoxide Dismutase
  • RNA, Messenger
  • Pregnancy
  • Placenta
  • Obstetrics & Reproductive Medicine
  • Keratins
  • In Situ Hybridization
  • Immunoenzyme Techniques
  • Hydrogen-Ion Concentration
 

Citation

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Chicago
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Boggess, K. A., Kay, H. H., Crapo, J. D., Moore, W. F., Suliman, H. B., & Oury, T. D. (2000). Differential localization of placental extracellular superoxide dismutase as pregnancy progresses. Am J Obstet Gynecol, 183(1), 199–205. https://doi.org/10.1067/mob.2000.105426
Boggess, K. A., H. H. Kay, J. D. Crapo, W. F. Moore, H. B. Suliman, and T. D. Oury. “Differential localization of placental extracellular superoxide dismutase as pregnancy progresses.Am J Obstet Gynecol 183, no. 1 (July 2000): 199–205. https://doi.org/10.1067/mob.2000.105426.
Boggess KA, Kay HH, Crapo JD, Moore WF, Suliman HB, Oury TD. Differential localization of placental extracellular superoxide dismutase as pregnancy progresses. Am J Obstet Gynecol. 2000 Jul;183(1):199–205.
Boggess, K. A., et al. “Differential localization of placental extracellular superoxide dismutase as pregnancy progresses.Am J Obstet Gynecol, vol. 183, no. 1, July 2000, pp. 199–205. Pubmed, doi:10.1067/mob.2000.105426.
Boggess KA, Kay HH, Crapo JD, Moore WF, Suliman HB, Oury TD. Differential localization of placental extracellular superoxide dismutase as pregnancy progresses. Am J Obstet Gynecol. 2000 Jul;183(1):199–205.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

July 2000

Volume

183

Issue

1

Start / End Page

199 / 205

Location

United States

Related Subject Headings

  • Trophoblasts
  • Superoxide Dismutase
  • RNA, Messenger
  • Pregnancy
  • Placenta
  • Obstetrics & Reproductive Medicine
  • Keratins
  • In Situ Hybridization
  • Immunoenzyme Techniques
  • Hydrogen-Ion Concentration