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TLR adaptor MyD88 is essential for pathogen control during oral toxoplasma gondii infection but not adaptive immunity induced by a vaccine strain of the parasite.

Publication ,  Journal Article
Sukhumavasi, W; Egan, CE; Warren, AL; Taylor, GA; Fox, BA; Bzik, DJ; Denkers, EY
Published in: J Immunol
September 1, 2008

TLR adaptor MyD88 activation is important in host resistance to Toxoplasma gondii during i.p. infection, but the function of this signaling pathway during oral infection, in which mucosal immunity assumes a predominant role, has not been examined. In this study, we show that MyD88(-/-) mice fail to control the parasite and succumb within 2 wk of oral infection. Early during infection, T cell IFN-gamma production, recruitment of neutrophils and induction of p47 GTPase IGTP (Irgm3) in the intestinal mucosa were dependent upon functional MyD88. Unexpectedly, these responses were MyD88-independent later during acute infection. In particular, CD4(+) T cell IFN-gamma reached normal levels independently of MyD88, despite continued absence of IL-12 in these animals. The i.p. vaccination of MyD88(-/-) mice with an avirulent T. gondii uracil auxotroph elicited robust IFN-gamma responses and protective immunity to challenge with a high virulence T. gondii strain. Our results demonstrate that MyD88 is required to control Toxoplasma infection, but that the parasite can trigger adaptive immunity without the need for this TLR adaptor molecule.

Duke Scholars

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

September 1, 2008

Volume

181

Issue

5

Start / End Page

3464 / 3473

Location

United States

Related Subject Headings

  • Toxoplasmosis
  • Toxoplasma
  • T-Lymphocytes
  • Protozoan Vaccines
  • Myeloid Differentiation Factor 88
  • Mice, Knockout
  • Mice
  • Intestinal Mucosa
  • Interferon-gamma
  • Immunology
 

Citation

APA
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ICMJE
MLA
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Sukhumavasi, W., Egan, C. E., Warren, A. L., Taylor, G. A., Fox, B. A., Bzik, D. J., & Denkers, E. Y. (2008). TLR adaptor MyD88 is essential for pathogen control during oral toxoplasma gondii infection but not adaptive immunity induced by a vaccine strain of the parasite. J Immunol, 181(5), 3464–3473. https://doi.org/10.4049/jimmunol.181.5.3464
Sukhumavasi, Woraporn, Charlotte E. Egan, Amy L. Warren, Gregory A. Taylor, Barbara A. Fox, David J. Bzik, and Eric Y. Denkers. “TLR adaptor MyD88 is essential for pathogen control during oral toxoplasma gondii infection but not adaptive immunity induced by a vaccine strain of the parasite.J Immunol 181, no. 5 (September 1, 2008): 3464–73. https://doi.org/10.4049/jimmunol.181.5.3464.
Sukhumavasi W, Egan CE, Warren AL, Taylor GA, Fox BA, Bzik DJ, et al. TLR adaptor MyD88 is essential for pathogen control during oral toxoplasma gondii infection but not adaptive immunity induced by a vaccine strain of the parasite. J Immunol. 2008 Sep 1;181(5):3464–73.
Sukhumavasi, Woraporn, et al. “TLR adaptor MyD88 is essential for pathogen control during oral toxoplasma gondii infection but not adaptive immunity induced by a vaccine strain of the parasite.J Immunol, vol. 181, no. 5, Sept. 2008, pp. 3464–73. Pubmed, doi:10.4049/jimmunol.181.5.3464.
Sukhumavasi W, Egan CE, Warren AL, Taylor GA, Fox BA, Bzik DJ, Denkers EY. TLR adaptor MyD88 is essential for pathogen control during oral toxoplasma gondii infection but not adaptive immunity induced by a vaccine strain of the parasite. J Immunol. 2008 Sep 1;181(5):3464–3473.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

September 1, 2008

Volume

181

Issue

5

Start / End Page

3464 / 3473

Location

United States

Related Subject Headings

  • Toxoplasmosis
  • Toxoplasma
  • T-Lymphocytes
  • Protozoan Vaccines
  • Myeloid Differentiation Factor 88
  • Mice, Knockout
  • Mice
  • Intestinal Mucosa
  • Interferon-gamma
  • Immunology