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Selective suppression of in vitro electrographic seizures by low-dose tetrodotoxin: a novel anticonvulsant effect.

Publication ,  Journal Article
Burack, MA; Stasheff, SF; Wilson, WA
Published in: Epilepsy research
October 1995

Localized injections of 50 microM tetrodotoxin (TTX) in rat hippocampal slices blocked stimulus train-evoked electrographic seizures (EGSs) for several hours. Responses to single stimuli were minimally altered during TTX block of the EGSs. This selective reduction of epileptiform activity could result from general blockade of action potentials in an anatomically distinct group of neurons in the slice. To test this hypothesis, we systematically mapped TTX injection sites in the hippocampal slice, and found that TTX injections that blocked EGSs were nearly always located in or invaded CA2/3 stratum radiatum and/or stratum lacunosum-moleculare. A high degree of recurrent activity in this region contributes to both epileptiform activity and responses to single stimuli; hence our selective inhibition of EGSs suggests a more pharmacologically specific anticonvulsant effect of TTX. Consistent with this hypothesis, we found that low concentrations of TTX (5, 10, or 20 nM) in the perfusion medium blocked EGSs without decreasing the amplitude of extracellular responses to single stimuli. Polysynaptic activity and/or antidromic firing may be particularly vulnerable to TTX action on voltage-gated sodium channels, due to their lower the safety factor for action potential propagation. Selective reduction of this activity may disrupt the abnormal neuronal activity underlying EGSs.

Duke Scholars

Published In

Epilepsy research

DOI

EISSN

1872-6844

ISSN

0920-1211

Publication Date

October 1995

Volume

22

Issue

2

Start / End Page

115 / 126

Related Subject Headings

  • Tetrodotoxin
  • Sodium Channels
  • Seizures
  • Rats, Sprague-Dawley
  • Rats
  • Neurology & Neurosurgery
  • Microinjections
  • Male
  • In Vitro Techniques
  • Hippocampus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Burack, M. A., Stasheff, S. F., & Wilson, W. A. (1995). Selective suppression of in vitro electrographic seizures by low-dose tetrodotoxin: a novel anticonvulsant effect. Epilepsy Research, 22(2), 115–126. https://doi.org/10.1016/0920-1211(95)00030-5
Burack, M. A., S. F. Stasheff, and W. A. Wilson. “Selective suppression of in vitro electrographic seizures by low-dose tetrodotoxin: a novel anticonvulsant effect.Epilepsy Research 22, no. 2 (October 1995): 115–26. https://doi.org/10.1016/0920-1211(95)00030-5.
Burack MA, Stasheff SF, Wilson WA. Selective suppression of in vitro electrographic seizures by low-dose tetrodotoxin: a novel anticonvulsant effect. Epilepsy research. 1995 Oct;22(2):115–26.
Burack, M. A., et al. “Selective suppression of in vitro electrographic seizures by low-dose tetrodotoxin: a novel anticonvulsant effect.Epilepsy Research, vol. 22, no. 2, Oct. 1995, pp. 115–26. Epmc, doi:10.1016/0920-1211(95)00030-5.
Burack MA, Stasheff SF, Wilson WA. Selective suppression of in vitro electrographic seizures by low-dose tetrodotoxin: a novel anticonvulsant effect. Epilepsy research. 1995 Oct;22(2):115–126.
Journal cover image

Published In

Epilepsy research

DOI

EISSN

1872-6844

ISSN

0920-1211

Publication Date

October 1995

Volume

22

Issue

2

Start / End Page

115 / 126

Related Subject Headings

  • Tetrodotoxin
  • Sodium Channels
  • Seizures
  • Rats, Sprague-Dawley
  • Rats
  • Neurology & Neurosurgery
  • Microinjections
  • Male
  • In Vitro Techniques
  • Hippocampus