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Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion.

Publication ,  Journal Article
Raucher, D; Stauffer, T; Chen, W; Shen, K; Guo, S; York, JD; Sheetz, MP; Meyer, T
Published in: Cell
January 21, 2000

Binding interactions between the plasma membrane and the cytoskeleton define cell functions such as cell shape, formation of cell processes, cell movement, and endocytosis. Here we use optical tweezers tether force measurements and show that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP2) acts as a second messenger that regulates the adhesion energy between the cytoskeleton and the plasma membrane. Receptor stimuli that hydrolyze PIP2 lowered adhesion energy, a process that could be mimicked by expressing PH domains that sequester PIP2 or by targeting a 5'-PIP2-phosphatase to the plasma membrane to selectively lower plasma membrane PIP2 concentration. Our study suggests that plasma membrane PIP2 controls dynamic membrane functions and cell shape by locally increasing and decreasing the adhesion between the actin-based cortical cytoskeleton and the plasma membrane.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

January 21, 2000

Volume

100

Issue

2

Start / End Page

221 / 228

Location

United States

Related Subject Headings

  • Stress, Mechanical
  • Signal Transduction
  • Second Messenger Systems
  • Protein Binding
  • Polymers
  • Phosphoric Monoester Hydrolases
  • Phosphatidylinositol 4,5-Diphosphate
  • Multienzyme Complexes
  • Mice
  • Membrane Proteins
 

Citation

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Raucher, D., Stauffer, T., Chen, W., Shen, K., Guo, S., York, J. D., … Meyer, T. (2000). Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell, 100(2), 221–228. https://doi.org/10.1016/s0092-8674(00)81560-3
Raucher, D., T. Stauffer, W. Chen, K. Shen, S. Guo, J. D. York, M. P. Sheetz, and T. Meyer. “Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion.Cell 100, no. 2 (January 21, 2000): 221–28. https://doi.org/10.1016/s0092-8674(00)81560-3.
Raucher D, Stauffer T, Chen W, Shen K, Guo S, York JD, et al. Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell. 2000 Jan 21;100(2):221–8.
Raucher, D., et al. “Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion.Cell, vol. 100, no. 2, Jan. 2000, pp. 221–28. Pubmed, doi:10.1016/s0092-8674(00)81560-3.
Raucher D, Stauffer T, Chen W, Shen K, Guo S, York JD, Sheetz MP, Meyer T. Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell. 2000 Jan 21;100(2):221–228.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

January 21, 2000

Volume

100

Issue

2

Start / End Page

221 / 228

Location

United States

Related Subject Headings

  • Stress, Mechanical
  • Signal Transduction
  • Second Messenger Systems
  • Protein Binding
  • Polymers
  • Phosphoric Monoester Hydrolases
  • Phosphatidylinositol 4,5-Diphosphate
  • Multienzyme Complexes
  • Mice
  • Membrane Proteins