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The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs.

Publication ,  Journal Article
Rowland, CR; Lennon, DP; Caplan, AI; Guilak, F
Published in: Biomaterials
July 2013

Scaffolds fabricated from cartilage extracellular matrix provide a chondroinductive environment that stimulates cartilaginous matrix synthesis in a variety of cell types. A limitation of these cartilage-derived matrix (CDM) scaffolds is that they contract during in vitro culture, which unpredictably alters their shape. The current study examined the hypothesis that collagen crosslinking techniques could inhibit cell-mediated contraction of CDM scaffolds. We analyzed the effects of dehydrothermal (DHT) treatment, ultraviolet light irradiation (UV), and the chemical crosslinker carbodiimide (CAR) on scaffold contraction and chondrogenic differentiation of adult human bone marrow-derived stem cells (MSCs). Both physical and chemical crosslinking treatments retained the original scaffold dimensions. DHT and UV treatments produced significantly higher glycosaminoglycan and collagen contents than CAR crosslinked and non-crosslinked constructs. Crosslinking treatments influenced the composition of newly synthesized matrix, and DHT treatment best matched the composition of native cartilage. DHT, UV, and non-crosslinked CDM films supported cell attachment, while CAR crosslinking inhibited cell adhesion. These results affirm that collagen crosslinking treatments can prevent cell-mediated contraction of CDM scaffolds. Interestingly, crosslinking treatments influence chondrogenic differentiation. These effects seem to be mediated by modifications to cell-matrix interactions between MSCs and the CDM; however, further work is necessary to elucidate the specific mechanisms involved in this process.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

July 2013

Volume

34

Issue

23

Start / End Page

5802 / 5812

Related Subject Headings

  • Transforming Growth Factor beta3
  • Tissue Scaffolds
  • Tissue Engineering
  • Sus scrofa
  • Porosity
  • Mesenchymal Stem Cells
  • Immunohistochemistry
  • Humans
  • Green Fluorescent Proteins
  • Glycosaminoglycans
 

Citation

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Rowland, C. R., Lennon, D. P., Caplan, A. I., & Guilak, F. (2013). The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs. Biomaterials, 34(23), 5802–5812. https://doi.org/10.1016/j.biomaterials.2013.04.027
Rowland, Christopher R., Donald P. Lennon, Arnold I. Caplan, and Farshid Guilak. “The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs.Biomaterials 34, no. 23 (July 2013): 5802–12. https://doi.org/10.1016/j.biomaterials.2013.04.027.
Rowland CR, Lennon DP, Caplan AI, Guilak F. The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs. Biomaterials. 2013 Jul;34(23):5802–12.
Rowland, Christopher R., et al. “The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs.Biomaterials, vol. 34, no. 23, July 2013, pp. 5802–12. Epmc, doi:10.1016/j.biomaterials.2013.04.027.
Rowland CR, Lennon DP, Caplan AI, Guilak F. The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs. Biomaterials. 2013 Jul;34(23):5802–5812.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

July 2013

Volume

34

Issue

23

Start / End Page

5802 / 5812

Related Subject Headings

  • Transforming Growth Factor beta3
  • Tissue Scaffolds
  • Tissue Engineering
  • Sus scrofa
  • Porosity
  • Mesenchymal Stem Cells
  • Immunohistochemistry
  • Humans
  • Green Fluorescent Proteins
  • Glycosaminoglycans