Short-term integration of Cdc25 dynamics controls mitotic entry during Drosophila gastrulation.

Journal Article (Journal Article)

Cells commit to mitosis by abruptly activating the mitotic cyclin-Cdk complexes. During Drosophila gastrulation, mitosis is associated with the transcriptional activation of cdc25(string), a phosphatase that activates Cdk1. Here, we demonstrate that the switch-like entry into mitosis observed in the Drosophila embryo during the 14(th) mitotic cycle is timed by the dynamics of Cdc25(string) accumulation. The switch operates as a short-term integrator, a property that can improve the reliable control of timing of mitosis. The switch is independent of the positive feedback between Cdk1 and Cdc25(string) and of the double negative feedback between Cdk1 and Wee1. We propose that the properties of the mitotic switch are established by the out-of-equilibrium properties of the covalent modification cycle controlling Cdk1 activity. Such covalent modification cycles, triggered by transcriptional expression of the activating enzymes, might be a widespread strategy to obtain reliable and switch-like control of cell decisions.

Full Text

Duke Authors

Cited Authors

  • Di Talia, S; Wieschaus, EF

Published Date

  • April 17, 2012

Published In

Volume / Issue

  • 22 / 4

Start / End Page

  • 763 - 774

PubMed ID

  • 22483720

Pubmed Central ID

  • PMC3643212

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2012.01.019


  • eng

Conference Location

  • United States