In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.

Published

Journal Article

Microtubules (MTs) are cytoskeletal polymers that undergo dynamic instability, the stochastic transition between growth and shrinkage phases. MT dynamics are required for diverse cellular processes and, while intrinsic to tubulin, are highly regulated. However, little is known about how MT dynamics facilitate or are regulated by tissue biogenesis and differentiation. We imaged MT dynamics in a smooth muscle-like lineage in intact developing Caenorhabditis elegans. All aspects of MT dynamics change significantly as stem-like precursors exit mitosis and, secondarily, as they differentiate. We found that suppression, but not enhancement, of dynamics perturbs differentiated muscle function in vivo. Distinct ensembles of MT-associated proteins are specifically required for tissue biogenesis versus tissue function. A CLASP family MT stabilizer and the depolymerizing kinesin MCAK are differentially required for MT dynamics in the precursor or differentiated cells, respectively. All of these multidimensional phenotypic comparisons were facilitated by a data display method called the diamond graph.

Full Text

Duke Authors

Cited Authors

  • Lacroix, B; Bourdages, KG; Dorn, JF; Ihara, S; Sherwood, DR; Maddox, PS; Maddox, AS

Published Date

  • April 2014

Published In

Volume / Issue

  • 29 / 2

Start / End Page

  • 203 - 216

PubMed ID

  • 24780738

Pubmed Central ID

  • 24780738

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2014.03.007

Language

  • eng