Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.

Published

Journal Article

A body of evidence has shown that the control of E2F transcription factor activity is critical for determining cell cycle entry and cell proliferation. However, an understanding of the precise determinants of this control, including the role of other cell-cycle regulatory activities, has not been clearly defined. Here, recognizing that the contributions of individual regulatory components could be masked by heterogeneity in populations of cells, we model the potential roles of individual components together with the use of an integrated system to follow E2F dynamics at the single-cell level and in real time. These analyses reveal that crossing a threshold amplitude of E2F accumulation determines cell cycle commitment. Importantly, we find that Myc is critical in modulating the amplitude, whereas cyclin D/E activities have little effect on amplitude but do contribute to the modulation of duration of E2F activation, thereby affecting the pace of cell cycle progression.

Full Text

Duke Authors

Cited Authors

  • Dong, P; Maddali, MV; Srimani, JK; Thélot, F; Nevins, JR; Mathey-Prevot, B; You, L

Published Date

  • September 2014

Published In

Volume / Issue

  • 5 /

Start / End Page

  • 4750 -

PubMed ID

  • 25175461

Pubmed Central ID

  • 25175461

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms5750

Language

  • eng