Skip to main content
Journal cover image

Phagocytosis of cryptococcus neoformans by rat alveolar macrophages

Publication ,  Journal Article
Bolaños, B; Mitchell, TG
Published in: Medical Mycology
January 1, 1989

A radiolabeling method was developed to investigate the interaction in vitro between Cryptococcus neoformans and Lewis rat alveolar macrophages (AMφ AMφ were harvested by lung lavage, monolayers of adherent cells were established in wells of microtiter plates and [51Cr]-labeled yeast cells were added to the monolayers. After removal of extracellular yeasts, the adherent radioactivity associated with the AMφ was directly proportional both to the number of yeasts added and to the number of yeasts per AMφ as determined by microscopic examination of Giemsa-stained monolayers. Phagocytosis (attachment and/or ingestion) of radiolabeled C. neoformans by AMφ was a sensitive, quantitative and reproducible assay for the evaluation of the AMφC. neoformans interaction. AMφ were able to phagocytose encapsulated strains of C. neoformans. The extent of phagocytosis was inversely related to the capsule size. Normal rat serum (NRS) was an excellent source of opsonins for the ingestion. Inactivation of serum complement or depletion of C3 by affinity chromatography removed most of the opsonic activity of NRS. Specific antibodies against C. neoformans did not increase phagocytosis. © 1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Duke Scholars

Published In

Medical Mycology

DOI

ISSN

1369-3786

Publication Date

January 1, 1989

Volume

27

Issue

4

Start / End Page

203 / 217

Related Subject Headings

  • Microbiology
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 3107 Microbiology
  • 1108 Medical Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bolaños, B., & Mitchell, T. G. (1989). Phagocytosis of cryptococcus neoformans by rat alveolar macrophages. Medical Mycology, 27(4), 203–217. https://doi.org/10.1080/02681218980000291
Bolaños, B., and T. G. Mitchell. “Phagocytosis of cryptococcus neoformans by rat alveolar macrophages.” Medical Mycology 27, no. 4 (January 1, 1989): 203–17. https://doi.org/10.1080/02681218980000291.
Bolaños B, Mitchell TG. Phagocytosis of cryptococcus neoformans by rat alveolar macrophages. Medical Mycology. 1989 Jan 1;27(4):203–17.
Bolaños, B., and T. G. Mitchell. “Phagocytosis of cryptococcus neoformans by rat alveolar macrophages.” Medical Mycology, vol. 27, no. 4, Jan. 1989, pp. 203–17. Scopus, doi:10.1080/02681218980000291.
Bolaños B, Mitchell TG. Phagocytosis of cryptococcus neoformans by rat alveolar macrophages. Medical Mycology. 1989 Jan 1;27(4):203–217.
Journal cover image

Published In

Medical Mycology

DOI

ISSN

1369-3786

Publication Date

January 1, 1989

Volume

27

Issue

4

Start / End Page

203 / 217

Related Subject Headings

  • Microbiology
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 3107 Microbiology
  • 1108 Medical Microbiology