Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques.

Published

Journal Article

It is widely appreciated that effective human vaccines directed against viral pathogens elicit neutralizing antibodies (NAbs). The passive transfer of anti-HIV-1 NAbs conferring sterilizing immunity to macaques has been used to determine the plasma neutralization titers, which must be present at the time of exposure, to prevent acquisition of SIV/HIV chimeric virus (SHIV) infections. We administered five recently isolated potent and broadly acting anti-HIV neutralizing monoclonal antibodies (mAbs) to rhesus macaques and challenged them intrarectally 24 h later with either of two different R5-tropic SHIVs. By combining the results obtained from 60 challenged animals, we determined that the protective neutralization titer in plasma preventing virus infection in 50% of the exposed monkeys was relatively modest (∼1:100) and potentially achievable by vaccination.

Full Text

Duke Authors

Cited Authors

  • Shingai, M; Donau, OK; Plishka, RJ; Buckler-White, A; Mascola, JR; Nabel, GJ; Nason, MC; Montefiori, D; Moldt, B; Poignard, P; Diskin, R; Bjorkman, PJ; Eckhaus, MA; Klein, F; Mouquet, H; Cetrulo Lorenzi, JC; Gazumyan, A; Burton, DR; Nussenzweig, MC; Martin, MA; Nishimura, Y

Published Date

  • September 22, 2014

Published In

Volume / Issue

  • 211 / 10

Start / End Page

  • 2061 - 2074

PubMed ID

  • 25155019

Pubmed Central ID

  • 25155019

Electronic International Standard Serial Number (EISSN)

  • 1540-9538

Digital Object Identifier (DOI)

  • 10.1084/jem.20132494

Language

  • eng

Conference Location

  • United States